Results 21 to 30 of about 985,944 (229)

The Nucleic Acid Knowledgebase: a new portal for 3D structural information about nucleic acids

Nucleic Acids Research, 2023
The Nucleic Acid Knowledgebase (nakb.org) is a new data resource, updated weekly, for experimentally determined 3D structures containing DNA and/or RNA nucleic acid polymers and their biological assemblies. NAKB indexes nucleic acid-containing structures
Catherine L Lawson, Helen M Berman, Li Chen, Brinda K. Vallat, Craig L. Zirbel   +4 more
semanticscholar   +1 more source

Argonaute protein-based nucleic acid detection technology

Frontiers in Microbiology, 2023
It is vital to diagnose pathogens quickly and effectively in the research and treatment of disease. Argonaute (Ago) proteins are recently discovered nucleases with nucleic acid shearing activity that exhibit specific recognition properties beyond CRISPR ...
Zhiyun Wu, Li Yu, Weifeng Shi, Jinhong Ma   +3 more
doaj   +1 more source

Amyloid-associated nucleic acid hybridisation. [PDF]

PLoS ONE, 2011
Nucleic acids promote amyloid formation in diseases including Alzheimer's and Creutzfeldt-Jakob disease. However, it remains unclear whether the close interactions between amyloid and nucleic acid allow nucleic acid secondary structure to play a role in ...
Sebastian Braun, Christine Humphreys, Elizabeth Fraser, Andrea Brancale, Matthias Bochtler, Trevor C Dale   +5 more
doaj   +1 more source

A systematic investigation of key factors of nucleic acid precipitation toward optimized DNA/RNA isolation

BioTechniques, 2020
Nucleic acid precipitation is important for virtually all molecular biology investigations. However, despite its crucial role, a systematic study of the influence factors of nucleic acid precipitation has not been reported.
Yalin Li, Suxiang Chen, Nan Liu, Lixia Ma, Tao Wang, Rakesh N Veedu, Tao Li, Fengqiu Zhang, Huiyue Zhou, Xiang Cheng, Xumiao Jing   +10 more
doaj   +1 more source

Effects of Different Lengths of a Nucleic Acid Binding Region and Bound Nucleic Acids on the Phase Behavior and Purification Process of HBcAg Virus-Like Particles

Frontiers in Bioengineering and Biotechnology, 2022
Virus-like particles (VLPs) are macromolecular structures with great potential as vehicles for the targeted administration of functional molecules. Loaded with nucleic acids, VLPs are a promising approach for nanocarriers needed for gene therapy.
Angela Valentic, Jakob Müller, Jürgen Hubbuch   +2 more
doaj   +1 more source

Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA

Cell Reports, 2015
We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 Å resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution.
Yijin Liu, Alasdair D.J. Freeman, Anne-Cécile Déclais, Timothy J. Wilson, Anton Gartner, David M.J. Lilley   +5 more
doaj   +1 more source

Optimizing scoring function of protein-nucleic acid interactions with both affinity and specificity. [PDF]

PLoS ONE, 2013
Protein-nucleic acid (protein-DNA and protein-RNA) recognition is fundamental to the regulation of gene expression. Determination of the structures of the protein-nucleic acid recognition and insight into their interactions at molecular level are vital ...
Zhiqiang Yan, Jin Wang
doaj   +1 more source

Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

Micromachines, 2021
Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale.
Yi Qiao, Yuhan Luo, Naiyun Long, Yi Xing, Jing Tu   +4 more
doaj   +1 more source

Recent Progress and Opportunities for Nucleic Acid Aptamers

Life, 2021
Coined three decades ago, the term aptamer and directed evolution have now reached their maturity. The concept that nucleic acid could modulate the activity of target protein as ligand emerged from basic science studies of viruses.
J. Byun
semanticscholar   +1 more source

Classification of the nucleolytic ribozymes based upon catalytic mechanism [version 1; peer review: 3 approved]

F1000Research, 2019
The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2′-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater.
David M.J. Lilley
doaj   +1 more source