Efficiency of polymerase chain reaction processes: A stochastic model [PDF]
, 2006 A stochastic model for the efficiency of polymerase chain reaction (PCR) processes is presented. The model is based on the assumption that the i’th nucleotide incorporation on the DNA template takes τi seconds, where τi has an exponential distribution ...
Hassibi, Arjang, Sharif, Masoud
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Journal of Medical Case Reports, 2020 Background Determining the infectiousness of patients with coronavirus disease 2019 is crucial for patient management. Medical staff usually refer to the results of reverse transcription polymerase chain reaction tests in conjunction with clinical ...
Masafumi Kanamoto +3 more
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Eradication of Helicobacter pylori Infection Using 7-day PCR-based Tailored Therapy [PDF]
The Korean Journal of Helicobacter and Upper Gastrointestinal Research, 2023 Background/Aims Standard triple therapy (STT; proton pump inhibitor [PPI]+clarithromycin+amoxicillin) used for Helicobacter pylori (H. pylori) eradication has shown low treatment success rates in recent years, which is most likely attributable to ...
Youngwoo Kim +6 more
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Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt [PDF]
Veterinary World, 2016 Aim: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella ...
Hazem Mohammed Ibrahim +3 more
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Validation of a Polymerase Chain Reaction technique for Kidd blood group genotyping [PDF]
, 2015 The Kidd blood group antigens, Jkª and Jkᵇ , are two of the main surface markers which are found on the membrane of red blood cells. The determination of whether a donor or a recipient has the Jkª and/or the Jkᵇ antigens is crucially important to ...
Borg, Joseph J. +2 more
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Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription [PDF]
, 1991 Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to ...
Baltimore, David +2 more
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Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.
BioTechniques, 2013 Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro.
R. Horton, Z. Cai, S. Ho, L. Pease
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Rickettsia prowazekii and Real-time Polymerase Chain Reaction
Emerging Infectious Diseases, 2006 Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase
Sanela Svraka +4 more
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Comparison of PCR fingerprinting techniques for the discrimination of Salmonella enterica subsp. enterica serovar Weltevreden isolated from indigenous vegetables in Malaysia. [PDF]
, 2008 Salmonella enterica subsp. enterica (S.) serovar Weltevreden has emerged as a public health problem in many countries. Genomic DNA of S. Weltevreden from indigenous vegetables namely 'selom' (Oenanthe stolonifera), 'pegaga' (Centella asiatica), 'kesum ...
Awang Saleh, Noorzaleha +5 more
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Laboratory Detection of Novel Corona Virus 2019 Using Polymerase Chain Reaction [PDF]
, 2020 Since mid-December 2019, several cases of a pneumonia like disease (with symptoms including fever, difficulty in breathing, cough and invasive lesions on both lungs) of unknown cause have emerged in the central Chinese city of Wuhan.
Chudhary, S. A. (Shafiq) +2 more
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