Results 31 to 40 of about 3,169,439 (400)
Comprehensive Optimization of Western Blotting
Gels, 2023 Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results. Therefore, we modified the steps of gel preparation, electrophoresis, electrotransfer, blocking, and gel cutting.
Dishiwen Liu +3 more
openaire +3 more sources
BioMed Research International, 2019 Densitometry data generated for Western blots are commonly used to compare protein abundance between samples. In the last decade, it has become apparent that assumptions underpinning these comparisons are often violated in studies reporting Western blot ...
Trent A Butler +4 more
semanticscholar +1 more source
Journal of Pharmaceutical Analysis, 2017 Monoclonal antibodies (MAbs) are important tools for the study of proteins′ function and structure. But there has been no report on the preparation of MAbs against human KIAA0100 protein up to date.
He Cui +4 more
doaj +1 more source
Antibody validation for Western blot: By the user, for the user
Journal of Biological Chemistry, 2019 Well-characterized antibody reagents play a key role in the reproducibility of research findings, and inconsistent antibody performance leads to variability in Western blotting and other immunoassays.
Lakshmi Pillai-Kastoori +5 more
semanticscholar +1 more source
Pathogens, 2023 The current Centers for Disease Control and Prevention (CDC) interpretive criteria for serodiagnosis of Lyme disease (LD) involve a two-tiered approach, consisting of a first-tier EIA, IFA, or chemiluminescent assay, followed by confirmation of positive ...
Richard Porwancher +2 more
doaj +1 more source
Expression of the DNA mismatch repair proteins hMLH1 and hPMS2 in normal human tissues. [PDF]
, 1997 hMLH1 and hPMS2 are part of the DNA mismatch repair complex. Mutations in these genes have been linked to hereditary non-polyposis colon cancer; they also occur in a variety of sporadic cancers. Western blot analysis and immunohistochemistry demonstrated
Aebi, S +6 more
core +1 more source
The Cell-Cycle Regulatory Protein p21CIP1/WAF1 Is Required for Cytolethal Distending Toxin (Cdt)-Induced Apoptosis. [PDF]
, 2020 The Aggregatibacter actinomycetemcomitans cytolethal distending toxin (Cdt) induces lymphocytes to undergo cell-cycle arrest and apoptosis; toxicity is dependent upon the active Cdt subunit, CdtB.
Boesze-Battaglia, Kathleen +4 more
core +2 more sources
In Situ Single-Cell Western Blot on Adherent Cell Culture.
Angewandte Chemie, 2019 Integrating 2D culture of adherent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design to eliminate trypsin release of cells to suspension, prior to single-cell isolation and protein analysis.
Yizhe Zhang, I. Naguro, A. Herr
semanticscholar +1 more source
Pathogens, 2022 Toxoplasma gondii is the causative agent of toxoplasmosis in humans and animals. The sexual reproductive cycle of Toxoplasma takes place in the small intestine of felines, the definitive hosts. In the final part of the sexual cycle, T.
María de la Luz Galván-Ramírez +6 more
doaj +1 more source
MiR-33a Controls hMSCS Osteoblast Commitment Modulating the Yap/Taz Expression Through EGFR Signaling Regulation [PDF]
, 2019 Mesenchymal stromal cells (hMSCs) display a pleiotropic function in bone regeneration. The signaling involved in osteoblast commitment is still not completely understood, and that determines the failure of current therapies being used.
Alessandro, Riccardo +9 more
core +1 more source