Results 41 to 50 of about 1,987,921 (339)

Introduction of Digital Polymerase Chain Reaction: Comparison with Real-time Polymerase Chain Reaction

Korean Journal of Clinical Laboratory Science
Digital polymerase chain reaction (dPCR) is an advanced molecular biology technique that provides a precise and absolute quantitation of target nucleic acids, providing significant improvements over traditional real-time PCR (qPCR) methods.
Dongsup LEE
doaj   +1 more source

ML Estimation of DNA Initial Copy Number in Polymerase Chain Reaction (PCR) Processes [PDF]

, 2007
Estimation of DNA copy number in a given biological sample is an extremely important problem in genomics. This problem is especially challenging when the number of the DNA strands is minuscule, which is often the case in applications such as pathogen and
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

Actinobaculum suis Detection Using Polymerase Chain Reaction

The Scientific World Journal, 2012
Actinobaculum suis is an important agent related to urinary infection in swine females. Due to its fastidious growth characteristics, the isolation of this anaerobic bacterium is difficult, thus impairing the estimation of its prevalence.
Cristina Román Amigo, Debora Dirani Sena de Gobbi, Vasco Túlio de Moura Gomes, Danilo do Prado Perina, Pedro Henrique Nogueira de Lima Filsner, Barbara Letícia Pereira Costa, Maria Garcia Spindola, Thais Sebastiana Porfida Ferreira, Paulo Eduardo Brandão, Andrea Micke Moreno   +9 more
doaj   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

RAPID DETECTION OF Salmonella IN SHRIMP BY POLYMERASE CHAIN REACTION [Deteksi Cepat Salmonella pada Udang dengan Polymerase Chain Reaction]

Jurnal Teknologi dan Industri Pangan, 2014
Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp.
Ulfah Amalia, Ratih Dewanti-Hariyadi*, Achmad Poernomo   +2 more
doaj   +1 more source

Optimasi Pcr (Polymerase Chain Reaction) Fragmen 724 Pb Gen Katg Multi Drug Resistance Tuberculosis Untuk Meningkatkan Produk Amplifikasi [PDF]

, 2013
Deteksi adanya mutasi pada gen katG MDR-TB (Multi Drug Resistance Tuberculosis) yang bertanggung jawab terhadap resistensi isoniazid (INH) dapat dilakukan dengan teknik Polymerase Chain Reaction (PCR).
C, Y. S. (Yowani), K, R. (Ratnayani), W, D. N. (Deniariasih)   +2 more
core  

Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma [PDF]

, 2015
Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy.
Asadi, J., Ghanbari, R., Knuutila, S., Malekzadeh, R., Mohamadkhani, A., Mosakhani, N., Mowla, S.J., Nouraee, N., Poustchi, H., Yazdani, Y.   +9 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Effects of upconversion nanoparticles on polymerase chain reaction. [PDF]

PLoS ONE, 2013
Nanoparticles (NPs) are attractive materials owing to their physical and electrochemical properties, which make them extremely useful in diagnostic applications.
Sang-Hyun Hwang, Su-Gyeong Im, Sang Soo Hah, Vu Thanh Cong, Eun Jeong Lee, Yeon-Su Lee, Geon Kook Lee, Do-Hoon Lee, Sang Jun Son   +8 more
doaj   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source