Results 141 to 150 of about 4,504,147 (220)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Electro-acoustic coupling of sequential pulsed electric field-ultrasound treatment: A novel technique for modification of protein isolate extracted from Capsicum annum L. seeds. [PDF]

open access: yesUltrason Sonochem
Manzoor MF   +8 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Can laryngeal ultrasound detect laryngeal movements during mechanically assisted cough? [PDF]

open access: yesERJ Open Res
Andersen TM   +7 more
europepmc   +1 more source

El Patrimoni arqueològic i el seu ús integrat

open access: yes
Descripció de l’estat dels jaciments arqueològics de l’alta Garrotxa, i proposta d’establiment d’un Parc cultural de la regió, “com un espai on la conservació, l’estudi i l"ús integrat del patrimoni cultural es converteixin en unes eines eficaces per al ...
Alcalde, Gabriel, Sañá Seguí, María
core  

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Disseny i càlcul d’una plataforma flotant per ús d’espectacles

open access: yes
L'objectiu d'aquest treball és realitzar el disseny i càlcul d'una plataforma flotant per ús d'espectacles. En ell es realitzen diferents estudis per tal de trobar una estructura capaç de suportar tot el pes que hi ha sobre la plataforma i que, a la ...
Abelló Gàllego, Cristina
core  

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