Results 31 to 40 of about 48,930 (267)
A high-performance thin-layer chromatography (HPTLC) method was developed for quantification of α-amylase inhibitory activity and stigmasterol content in ant plant extracts.
Snezana Agatonovic-Kustrin +1 more
doaj +1 more source
Multispectroscopy analysis of polystyrene nanoplastic interaction with diastase α-amylase
The digestive enzyme of plant are generally α-amylase. They functions enzyme that breakdown starch into maltose and sugars. This happens in the endosperm of the seed.
Ananthaselvam Azhagesan +2 more
doaj +1 more source
α-amylase was produced by Aspergillus terreus NCFT 4269.10 using both liquid static surface (LSSF) and solid-state fermentation using pearl millet residues as substrate. The maximum production of α-amylase was noticed at 30°C incubated for 96h. The crude
Bijay Kumar Sethi +3 more
doaj +1 more source
Cloning and expression of an extracellular α-amylase gene fromStreptomyces hygroscopicusinStreptomyces lividans66 [PDF]
An extracellular α-amylase gene was cloned from Streptomyces hygroscopicus into Streptomyces lividans 66 using the multicopy vector pIJ702. Subcloning of DNA from the original clone allowed the gene to be localized to a 2.8 kb segment of DNA. Use of a promoter probe vector gave a possible location of the gene promoter and also the direction of ...
Christine McKillop +2 more
openaire +1 more source
Background: Diabetes mellitus is a metabolic disease, characterized by hyperglycemia due to disturbance in both insulin secretion and function. One of theurapeutic approaches is to reduce blood glucose levels by inhbiting α-/β-glucosidase and α-amylase ...
Marisca Evalina Gondokesumo +2 more
doaj +1 more source
Changes in the cell envelope structure ofClostridiumsp. strain EM1 during massive production of α-amylase and pullulanase [PDF]
Marked changes in cell envelope structure were observed when Clostridium sp. strain EM1 cells grown in continuous culture under glucose limitation were compared with cells grown under starch limitation. The increase in the level of extracellular α-amylase and pullulanase during starch-limited growth was parallelled by degradation of the cell-envelope ...
G. Antranikian +3 more
openaire +1 more source
Cloning and expression of the thermostable α-amylase gene fromClostridium thermosulfurogenes(DSM 3896) inEscherichia coli [PDF]
The gene coding for a thermostable α-amylase from Clostridium thermosulfurogenes (DSM 3896) was cloned in Escherichia coli using pUC18 as a vector. The recombinant plasmid pCT2 of an amylolytic positive transformant of E. coli contained a 2.9 kbp fragment of chromosomal DNA of C. thermosulforogenes carrying the α-amylase gene. In E.
K. Haeckel, H. Bahl
openaire +1 more source
Bread Spoilage Fungi as Creators of α Amylase Using Two Types of Wheat Flour
Fungal spoilage of bread can be a great problem; however, it can be explored as a producer of enzymes. The fungi were isolated from breads, and their activity for α-amylase production was planned.
Aisha M. H. Al-Rajhi +4 more
doaj +2 more sources
Construction of α-amylase-producing strains not subject to carbon catabolite repression [PDF]
The dyatic symmetric element (DSE) present in the alpha-amylase gene promoter region of the thermophilic Streptomyces strain sp. TO1, and the whole alpha-amylase gene (amy TO1) with a 3-bp change in the DSE, were cloned in the high copy number replicative cloning vector pIJ702 giving pLM10 and pLM11 plasmids respectively.
Lotfi, Mellouli +2 more
openaire +2 more sources
Electrically Readable Lateral Flow Assay Using Organic Transistors for Diagnostic Applications
Electrolyte‐gated organic field‐effect transistors (EGOFETs) are integrated with lateral flow (LF) paper fluidics to create a reusable, portable, and low‐cost point‐of‐care (PoC) diagnostic test. The devices are validated for Human Immunoglobulin G detection, achieving high sensitivity (0.1 fm), selectivity, and reproducibility with rapid results in 20–
María Jesús Ortiz‐Aguayo +4 more
wiley +1 more source

