Results 21 to 30 of about 90,634 (287)

Characterization of β-galactosidase and α-galactosidase activities from the halophilic bacterium Gracilibacillus dipsosauri

Annals of Microbiology, 2021
Purpose Gracilibacillus dipsosauri strain DD1 is a salt-tolerant Gram-positive bacterium that can hydrolyze the synthetic substrates o-nitrophenyl-β-d-galactopyranoside (β-ONP-galactose) and p-nitrophenyl-α-d-galactopyranoside (α-PNP-galactose).
Charles E. Deutch, Amy M. Farden, Emily S. DiCesare   +2 more
doaj   +1 more source

Genomic Features of a Food-Derived Pseudomonas aeruginosa Strain PAEM and Biofilm-Associated Gene Expression under a Marine Bacterial α-Galactosidase. [PDF]

Int J Mol Sci, 2020
Balabanova L, Shkryl Y, Slepchenko L, Cheraneva D, Podvolotskaya A, Bakunina I, Nedashkovskaya O, Son O, Tekutyeva L.   +8 more
europepmc   +3 more sources

Role of glycosylation in secretion and stability of micromycetes α-galactosidase [PDF]

The Ukrainian Biochemical Journal, 2014
The effect of the glycosylation inhibitors (tunicamycin and 2-deoxy-D-glucose) on the activity, stabili­ty and production of fungal glycosidases has been studied.
N. V. Borzova, O. V. Gudzenko, L. D. Varbanets   +2 more
doaj   +1 more source

Characterization and molecular cloning of a heterodimeric β-galactosidase from the probiotic strainLactobacillus acidophilusR22 [PDF]

FEMS Microbiology Letters, 2007
Beta-galactosidase from the probiotic strain Lactobacillus acidophilus R22 was purified to apparent homogeneity by ammonium sulphate fractionation, hydrophobic interaction, and affinity chromatography. The enzyme is a heterodimer consisting of two subunits of 35 and 72 kDa, as determined by gel electrophoresis.
Thu-Ha, Nguyen, Barbara, Splechtna, Stanimira, Krasteva, Wolfgang, Kneifel, Klaus D, Kulbe, Christina, Divne, Dietmar, Haltrich   +6 more
openaire   +2 more sources

α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]

FEMS Microbiology Letters, 2000
A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead, M P, Fransen, R Y, Eberhart, A J, Park, H J, Gilbert, G P, Hazlewood   +5 more
openaire   +2 more sources

Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]

FEMS Microbiology Letters, 2008
A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao, Lili, Lu, Min, Xiao, Qinpeng, Wang, Yu, Lu, Chunhui, Liu, Peng, Wang, Hidehiko, Kumagai, Kenji, Yamamoto   +8 more
openaire   +2 more sources

Oral Migalastat HCl Leads to Greater Systemic Exposure and Tissue Levels of Active α-Galactosidase A in Fabry Patients when Co-Administered with Infused Agalsidase. [PDF]

, 2015
UnlabelledMigalastat HCl (AT1001, 1-Deoxygalactonojirimycin) is an investigational pharmacological chaperone for the treatment of α-galactosidase A (α-Gal A) deficiency, which leads to Fabry disease, an X-linked, lysosomal storage disorder. The currently
Adera, Mathews, Barlow, Carrolee, Barth, Jay, Bichet, Daniel G, Boudes, Pol, Eyskens, Francois, Flanagan, John J, Goker-Alpan, Ozlem, Holida, Myrl, Johnson, Franklin K, Khanna, Richie, Lockhart, David J, Nicholls, Kathy, Shankar, Suma, Sitaraman, Sheela, Thomas, Mark, Valenzano, Kenneth J, Warnock, David G, Wustman, Brandon A   +18 more
core   +8 more sources

Effect of β-Mannanase and α-Galactosidase Supplementation to Soybean Meal Based Diets on Growth, Feed Efficiency and Nutrient Digestibility of Rainbow Trout, (Walbaum) [PDF]

Asian-Australasian Journal of Animal Sciences, 2014
A 12-week feeding trial was conducted with 87 g rainbow trout to evaluate the effects on growth performances, feed efficiency and nutrient digestibility of adding β-mannanase and α-galactosidase enzymes, solely or in combination.
Nalan Ozgur Yiğit, Seval Bahadir Koca, Behire Isıl Didinen, Ibrahim Diler   +3 more
doaj   +1 more source

High gene expression inEscherichia coliof recombinant alginate lyase as a fused protein with β-galactosidase α-peptide [PDF]

FEMS Microbiology Letters, 1995
Escherichia coli LE392 (pAL28) was previously isolated as a positive clone harboring the alginate lyase gene (aly) from an alginate-degrading strain, Pseudomonas sp. OS-ALG-9. The plasmid pAL205, one of the constructs obtained after successive subcloning of pAL28, gave the highest expression of aly in E. coli cells.
K, Fujiyama, H, Maki, S, Kinoshita, T, Yoshida   +3 more
openaire   +2 more sources

Immobilization of the Antarctic sp. LX-1 α-Galactosidase on Eudragit L-100 for the Production of a Functional Feed Additive [PDF]

Asian-Australasian Journal of Animal Sciences, 2013
Partially purified α-galactosidase from Bacillus sp. LX-1 was non-covalently immobilized on a reversibly soluble-insoluble polymer, Eudragit L-100, and an immobilization efficiency of 0.93 was obtained.
Jaekoo Lee, Inkyung Park, Jaiesoon Cho
doaj   +1 more source