Results 31 to 40 of about 4,325 (125)

Deep Straw Incorporation With Supplemental Fertilization Improves Subsoil Quality and Maize Productivity

Land Degradation &Development, EarlyView.
ABSTRACT Deep straw incorporation is widely advocated for enhancing crop yields and improving agroecosystem functions and services. However, how this practice interacts with different fertilization strategies synergistically to influence soil quality and maize productivity remains unclear.
Jian Chen, Jianxuan Cai, Zhengjun Yan, Yiping Xu, Huiqing Shangguan, Hongshuo Wang, Shunli Zhou, Yuan Wen   +7 more
wiley   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

The FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti, Andre Schutzer Godoy, Caio Cesar Mello Capetti, Rafaela Sachetto Fernandes, Vanessa O. A. Pellegrini, Ornella M. Ontañon, Eleonora Campos, Rodrigo V. Portugal, Igor Polikarpov   +8 more
wiley   +1 more source

Molecular cloning, expression, and characterization of four novel thermo-alkaliphilic enzymes retrieved from a metagenomic library

Biotechnology for Biofuels, 2017
Background Enzyme discovery is a promising approach to aid in the deconstruction of recalcitrant plant biomass in an industrial process. Novel enzymes can be readily discovered by applying metagenomics on whole microbiomes.
Mukil Maruthamuthu, Jan Dirk van Elsas
doaj   +1 more source

Structural insights into xyloglucan recognition by an ABC transporter from a Gram‐positive, thermophilic bacterium

The FEBS Journal, EarlyView.
Athe_2052 is an ABC transporter substrate‐binding protein that recognizes xyloglucan heptasaccharide (XXXG), a soluble breakdown product of plant xyloglucan. The crystal structure of Athe_2052, together with binding assays and computational modeling, shows how XXXG is recognized by this sugar transporter.
Hansen Tjo, Virginia Jiang, Philip D. Jeffrey, Angela Zhu, A. James Link, Jerelle A. Joseph, Jonathan M. Conway   +6 more
wiley   +1 more source

Reassigning the role of a mesophilic xylan hydrolysing family GH43 β-xylosidase from Bacteroides ovatus, BoExXyl43A as exo-β-1,4-xylosidase

Current Research in Biotechnology
The recombinant 40 kDa BoExXyl43A glycoside hydrolase family 43 (GH43) from bacterium Bacteroides ovatus exhibited highest specific activity (U/mg) against corn cob xylan (136.8), followed by Beechwood xylan (81.1), Carbosynth xylan (69.3), 4-O-D ...
Parmeshwar Vitthal Gavande, Shyam Ji, Vânia Cardoso, Carlos M.G.A. Fontes, Arun Goyal   +4 more
doaj   +1 more source

A multifunctional GH39 glycoside hydrolase from the anaerobic gut fungus Orpinomyces sp. strain C1A [PDF]

PeerJ, 2016
Background. The anaerobic gut fungi (phylum Neocallimastigomycota) represent a promising source of novel lignocellulolytic enzymes. Here, we report on the cloning, expression, and characterization of a glycoside hydrolase family 39 (GH39) enzyme (Bgxg1 ...
Jessica M. Morrison, Mostafa S. Elshahed, Noha Youssef   +2 more
doaj   +2 more sources

Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme

Plant Biotechnology Journal, EarlyView.
ABSTRACT Fruit cuticles regulate the diffusion of water, gases and solutes, functioning as essential protective interfaces against environmental and biotic stresses. Engineering cuticle structure and mechanics therefore represents a promising strategy for improving fruit quality and resilience.
Gulab Chand Arya, Ekram Wassel, Vibha Mishra, Ekaterina Manasherova, Ruth E. Stark, Hagai Cohen   +5 more
wiley   +1 more source

Sugars as Central Integrators of Plant Immunity and Stress Responses

Plant, Cell &Environment, EarlyView.
ABSTRACT Sugars are fundamental metabolites that sustain plant growth and development, but accumulating evidence demonstrates that they also function as regulatory hubs in plant defence. In this review, we synthesise current knowledge on how carbohydrate dynamics coordinate metabolism, signalling, and structural responses to shape plant resistance ...
Anna Wlazło, Anna Barczak‐Brzyżek, Marcin Filipecki   +2 more
wiley   +1 more source

Plant genotypic diversity and defoliation jointly shape root exudation, fungal communities, and carbon and nitrogen cycling

New Phytologist, Volume 252, Issue 1, Page 324-339, October 2026.
Experimental design. Summary Intraspecific plant diversity is a major component of community diversity, yet its role in shaping plant–microbial interactions and soil functioning remains poorly understood. In particular, plant genotypic diversity may modulate ecosystem responses to defoliation, a key driver of ecosystem processes in grazed systems.
Irene Cordero, Mathilde Chomel, Ellen L. Fry, Agnès Ardanuy, Ully Kritzler, Hayley Craig, Cristina Heredia‐Acuña, Sarah Worthington, Stênio I. A. Foerster, Richard D. Bardgett, David Johnson, Marina Semchenko   +11 more
wiley   +1 more source

Characterization of β-glucosidase activity of a Lactiplantibacillus plantarum 6-phospho-β-glucosidase

Applied Microbiology and Biotechnology
β-Glucosidases are useful for hydrolysis of glycosidically-bound volatiles (GBV), thereby facilitating the release of aroma chemicals from the fruit matrices. In this study, 10 putative glycosyl hydrolases belonging to GH1 family from Lactiplantibacillus
Ravish Godse, Joyleen M. Fernandes, Ram Kulkarni   +2 more
doaj   +1 more source