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Integrative Conservation, Volume 4, Issue 3, Page 277-286, September 2025.This study provides an innovation to address the challenge in accessibility to samples and genetic markers in Snow leopard conservation genetic studies. We performed next‐generation sequencing (NGS) on 19 fecal samples obtained from the eastern Qinghai‐Tibet Plateau, and successfully assembled 17 complete mitogenome sequences (~16,720 bp) and ...
Chen Cheng +7 more
wiley +1 more source
Veterinary Dermatology, Volume 36, Issue 4, Page 474-484, August 2025.Background – Sporotrichosis is a chronic, mycotic infection caused by fungi of the genus Sporothrix. Zoonotic sporotrichosis occurs mainly through S. brasiliensis transmission, resulting from the organism's traumatic introduction via scratches or bites, or contact with exudate from contaminated cats.
Steffanie Amadei +6 more
wiley +1 more source
Veterinary Dermatology, Volume 36, Issue 3, Page 302-313, June 2025.Background – Malassezia pachydermatis (MP) is implicated in severe dermatitis and otitis externa (OE) of companion animals and recently gained attention for its increasing resistance to azole compounds. For this reason, developing novel therapeutic strategies is of great interest. In a previous work, we used reference yeast isolates to evaluate several
Costanza Spadini +11 more
wiley +1 more source
Study on establisment of chicken embryo RNAi technology with infective feature of RCAS and Vezf1 function in chicken vascular development. [PDF]
, 2012 早期初级血管网络的形成，始于胚胎中胚层形成过程中其部分细胞分化为血管内皮前体细胞。血管内皮前体细胞再进一步向血管内皮细胞分化并形成初级毛细血管网，血管内皮细胞与其周围细胞进一步相互作用，逐步进行对初级血管的改建和生成新的血管，最终完成血管系统的发育。因此，血管内皮细胞是血管生成的中心细胞。为研究胚胎血管内皮细胞分化机制，先前我们从发育至三体节的鸡内皮前体细胞（VEGFR2+）相对特异表达基因的克隆中，发现了鸡的Vezf1(cVezf1)基因。该基因与在小鼠中发现的Vezf1基因高度同源 ...
张超
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Physicochemical Characteristics and Spoilage Bacteria of Modified Atmosphere Packaged Braised Chicken during Refrigeration [PDF]
, 2023 In order to explore dynamic changes in the physicochemical characteristics and microbial diversity of modified atmosphere packaged braised chicken during refrigeration, high-throughput sequencing technology and traditional isolation and identification ...
ZHANG Li, MA Yunhao, WANG Ying, XU Baocai
core +1 more source
Preparation and Application of Monoclonal Antibody against Puerarin [PDF]
, 2012 葛根为豆科植物野葛或粉葛的干燥根，为常用中药之一，具有解肌退热、生津、透疹、升阳止泻的功效，多用于治疗外感发热、头痛、项强、消渴、麻疹不透、热痢、泄泻、高血压等症状。葛根素(Puerarin，PU)是葛根的主要活性成分，属于黄酮类化合物，药理作用广泛，是一种很有开发前景的药物。 本实验采用杂交瘤技术制备葛根素的单克隆抗体，在此基础上建立葛根素的酶联免疫吸附分析法检测技术，对葛根中药及中成药中葛根素的含量进行检测和评价。 主要的实验结果如下： （1）采用高碘酸钠法合成免疫人工抗原PU ...
吴俊杰
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Veterinary Dermatology, Volume 35, Issue 5, Page 479-491, October 2024.Background – Lop‐eared rabbits may be predisposed to otitis externa (OE) as a consequence of their ear conformation. Although otoscopy, otic cytological evaluation, and culture are valuable tools in dogs and cats, published data on rabbits remain lacking. Hypothesis/Objectives – This study aimed to assess the utility of otoscopy and cytological results
Nikoleta Makri +8 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2009 目的建立基于SELDI-TOF-MS技术的蛋白质分离、纯化、鉴定技术,用于鉴定运用SELDI技术发现的肝硬化相关血浆差异蛋白。方法采用尿素预处理血液样本,结合离子交换层析、SELDI芯片检测及芯片上酶解、质谱鉴定等多种技术手段,对肝硬化相关的差异蛋白质进行了分离、纯化和鉴定。结果选用阳离子交换剂对血浆样品进行分离,并对分离的各组分进行芯片检测,在300 mM NaCl洗脱组分中,含有与目标蛋白荷质比相同、理化性质相同的蛋白质峰,并且该目标蛋白得到富集 ...
陆晔 +4 more
doaj
野生动物学报, 2023 为探究林麝（Moschus berezovskii）的死亡原因，无菌采集死亡林麝脏器样本，通过细菌分离纯化、生化鉴定、16S rDNA基因测序、纸片扩散法和耐药基因检测方法对细菌进行分离、鉴定及分析分离菌株的耐药性。结果表明：从死亡林麝脏器样本中分离得到的菌株为弗格森埃希菌（Escherichia fergusonii），该分离菌株对头孢噻肟、头孢噻吩和青霉素耐药，对氨基糖苷类、喹诺酮类及氨基青霉素类药物敏感；携带耐药基因bla-OXA1/4/30、bla-CTX-M和bla-SHV ...
彭茜翎 +9 more
doaj
Studies on Kinmen kaoliag liquor brewing system and the application of its secondary production- yellow water [PDF]
, 2015 在酿酒过程中，高粱是决定白酒质量优劣的重要因素。高粱中淀粉含量越多，出酒率越高。除了淀粉外，高粱果皮中单宁和籽粒中蛋白质、脂肪等，在发酵过程中会产生一些副产物，从而影响高粱酒的风味。本研究选了金选、两糯、兴湘、红梗和红糯等5个品种的高粱进行外观、生化性质等传统研究，结果表明两糯总淀粉及支链淀粉含量最高，金选单宁含量最高。为能分辨不同高粱品种，收集了5个不同品种的高粱作为实验样本，测定它们的近红外光谱，利用光谱特征对其进行定性定量分析，可快速鉴别出5种高粱之间的品种差异。利用高粱品种间SSR ...
陳志敏
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