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抗胰腺癌单克隆抗体的研究 [PDF]

Zhongshan Daxue xuebao. Yixue kexue ban, 1989
用人新鲜胰腺癌组织免疫BALB/c小鼠,取脾细胞与小鼠骨髓瘤细胞Sp2/0-Agl4融合,获得了分泌抗人胰腺癌单克隆抗体的杂交瘤细胞株。本文重点报道以ABC免疫组织化学法检查YPC1单克隆抗体与人正常组织和肿瘤组织的反应性。结果表明YPC1单克隆抗体与16例被检测的胰腺癌组织发生反应。其中9例(56.3％)呈强阳性反应,与23种正常组织中的10种(43.5％)以及19种非胰腺肿瘤中的8种(42.1％)有不同程度的交叉反应,但多数反应较弱。本研究提示以新鲜人体肿瘤组织作为免疫原制备单克隆抗体是简便可行的 ...
doaj   +2 more sources

抗H7N9流感病毒的单克隆抗体与胃组织的交叉反应研究

Zhongguo shiyan zhenduanxue
目的 筛选鉴定与胃组织交叉反应的抗H7N9流感病毒的单克隆抗体，初步探讨流感病毒感染会引发肠道相关疾病的可能致病机制。方法 利用H7N9流感病毒制备了大量的单克隆抗体，对制备的单克隆抗体运用ELISA方法进行亚型和效价测定，免疫组化对制备的单克隆抗体与组织的反应性进行筛选，巢式PCR分子生物学技术克隆单克隆抗体轻重链可变区序列。结果 制备的单克隆抗体H7N9-71和H7N9-78均能与H7N9抗原特异性结合，抗体效价达1∶10～5,免疫组化结果表明H7N9-71、H7N9 ...
孙晶莹, 张海祥, 冯杨萌, 封青, 李研, 孙丽君, 徐翠香   +6 more
doaj   +1 more source

Fatty acid β‐oxidation and mitochondrial fusion are involved in cardiac microvascular endothelial cell protection induced by glucagon receptor antagonism in diabetic mice 脂肪酸β‐氧化和线粒体融合参与胰高血糖素受体拮抗剂对糖尿病小鼠心脏微血管内皮细胞的保护作用

Journal of Diabetes, Volume 15, Issue 12, Page 1081-1094, December 2023., 2023
Highlights Glucagon receptor (GCGR) monoclonal antibody (mAb) improves cardiac function and attenuates microvascular damage in db/db mice. GCGR mAb ameliorates the oxidative stress of cardiac microvascular endothelial cells in diabetic mice. GCGR mAb counteracts the diabetes‐induced upregulation of carnitine palmitoyltransferase 1B, optic atrophy type ...
Peng Wang, Rui Wei, Xiaona Cui, Zongzhe Jiang, Jin Yang, Lingyun Zu, Tianpei Hong   +6 more
wiley   +1 more source

Cytokine transcriptome profiling in acute experimental canine atopic dermatitis skin lesions after IL‐31 inhibition with lokivetmab

Veterinary Dermatology, Volume 34, Issue 4, Page 327-338, August 2023., 2023
Background – The caninised monoclonal antibody lokivetmab (LKV), directed at interleukin (IL)‐31, is very effective at controlling pruritus in most dogs with atopic dermatitis (AD). However, evidence exists that IL‐31 is not required for the induction of acute allergic skin inflammation, which might explain why this treatment is less efficacious in ...
Chie Tamamoto‐Mochizuki, Natalie Crawford, Jordan M. Eder, Andrea J. Gonzales, Thierry Olivry   +4 more
wiley   +1 more source

A masked, randomised clinical trial evaluating the efficacy and safety of lokivetmab compared to saline control in client‐owned dogs with allergic dermatitis

Veterinary Dermatology, Volume 32, Issue 5, Page 477-e131, October 2021., 2021
Background – Interleukin (IL)‐31 is an important mediator in canine atopic dermatitis (cAD) and also may be dysregulated in other allergic diseases. Hypothesis/Objectives – To demonstrate the efficacy and safety of lokivetmab (canine anti‐IL‐31 monoclonal antibody) for treatment of pruritus associated with allergic dermatitis in dogs.
Leen Van Brussel, Hilde Moyaert, Monica Escalada, Sean P. Mahabir, Michael R. Stegemann   +4 more
wiley   +1 more source

Detection and Inhibition of IgE for cross‐reactive carbohydrate determinants evident in an enzyme‐linked immunosorbent assay for detection of allergen‐specific IgE in the sera of dogs and cats

Veterinary Dermatology, Volume 31, Issue 6, Page 439-e116, December 2020., 2020
Background It has been demonstrated recently that immunoglobulin (Ig)E specific for cross‐reactive carbohydrate determinants (CCD) is present in the serum of allergen‐sensitized dogs and cats, and that these CCD‐specific antibodies might confound serological testing. Hypothesis/Objective The objective was to document the prevalence of CCD detectable in
Kenneth W. Lee, Brennan H. McKinney, Karen D. Blankenship, Daniel O. Morris   +3 more
wiley   +1 more source

Preparation and Application of Monoclonal Antibody against Puerarin [PDF]

, 2012
葛根为豆科植物野葛或粉葛的干燥根，为常用中药之一，具有解肌退热、生津、透疹、升阳止泻的功效，多用于治疗外感发热、头痛、项强、消渴、麻疹不透、热痢、泄泻、高血压等症状。葛根素(Puerarin，PU)是葛根的主要活性成分，属于黄酮类化合物，药理作用广泛，是一种很有开发前景的药物。 本实验采用杂交瘤技术制备葛根素的单克隆抗体，在此基础上建立葛根素的酶联免疫吸附分析法检测技术，对葛根中药及中成药中葛根素的含量进行检测和评价。 主要的实验结果如下： （1）采用高碘酸钠法合成免疫人工抗原PU ...
吴俊杰
core  

Evaluation of filaggrin 2 expression in dogs with atopic dermatitis before and after oclacitinib maleate administration

Veterinary Dermatology, Volume 36, Issue 4, Page 453-461, August 2025.
Background – Canine atopic dermatitis (cAD) is a chronic, inflammatory, multifactorial and pruritic disease. The presence of skin barrier impairment (e.g. filaggrin alterations), along with abnormal immune responses, can negatively impact cutaneous barrier function.
Wendie Roldan Villalobos, Tássia Ferreira, Fernanda Borek, Domenico Santoro, Lluis Ferrer, Marconi Farias   +5 more
wiley   +1 more source

Preparation of monoclonal antibodies against mouse alpha-fetoprotein and preliminary study on their application [PDF]

, 2016
原发性肝癌是我国高发的恶性肿瘤之一，每年新发和死亡患者均占全球总数的一半以上。根据全国肿瘤登记中心最新公布的2015年《肿瘤登记年报》数据，我国肝癌新增病例为46.61万，占所有恶性肿瘤的第4位（10.86%），而死亡病例为42.21万，占所有癌症死亡的第3位（15.00%），仅次于肺癌和胃癌。尽管近年来肝癌的诊断和治疗有了一定的进展，其预后仍然不理想，五年生存率极低。究其原因，大部分肝癌患者往往在疾病的晚期才被诊断出来，有些甚至发生了远端转移，因而错过了最佳治疗时机；此外 ...
潘海娇
core  

Long‐term use of lokivetmab in dogs with atopic dermatitis

Veterinary Dermatology, Volume 35, Issue 6, Page 683-693, December 2024.
Background – Lokivetmab, a caninised monoclonal antibody against interleukin (IL)‐31, is an effective treatment for the pruritus associated with canine atopic dermatitis (cAD). Objectives – To investigate the efficacy and safety of lokivetmab during long‐term treatment defined as at least three consecutive lokivetmab injections in atopic dogs under ...
Bettina Kasper, Yury Zablotski, Ralf S. Mueller   +2 more
wiley   +1 more source