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递呈BAC5 单抗识别位点的M13噬菌体克隆系的建立 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】获得可递呈抗低分化和未分化癌细胞单克隆抗体(BAC5 单抗)抗原表位的M13噬菌体克隆系。【方法】以 BAC5 单抗为靶抗体, 对由M13噬菌体构建的随机12 肽文库进行生物淘洗。用抗体竞争方法从阳性克隆中选出与BAC5 单抗结 合率较高的克隆系。通过ELISA 方法检测BAC5 单抗对上述克隆的选择性识别。【结果】经过3 轮淘洗, 获得的阳性克隆率为 77 %(35/ 45)。来自C2、C17 和C29 号克隆的噬菌体对外加BAC5 单抗的结合率分别为71.6 %、49.4 %和64.0%
肖锡宾 +5 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 1999 目的: 制备从尖吻蝮蛇蛇毒中分离的纤溶因子的单克隆抗体, 为克隆基因提供特异性的探针。方法: 将纤溶因 子免疫 Balb/C 小鼠,取脾细胞与 SP 2/0 骨髓瘤细胞融合, 筛选稳定分泌抗体的细胞株。采用血纤维蛋白平板法,观察单克隆 抗体能否抑制纤溶因子的纤溶作用。结果: 获得 2 株稳定分泌单克隆抗体的细胞株。 Western 印迹显示该单克隆抗体能特异 地与纤溶因子结合,与其它蛋白无交叉反应, 此外,这 2株单克隆抗体具有抑制纤溶因子溶解人血纤维蛋白的作用。结论: 这 2 ...
邱鹏新 黎明涛 苏兴文 陈家树 颜光美
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单克隆多克隆抗体不同标记ELISA技术对HBeAg/抗-HBe检测评估
Zhongshan Daxue xuebao. Yixue kexue ban, 1993 采用抗-HBe单克隆和多克隆抗体进行生物素或酶标记作酶免疫试剂检测HBeAg及抗-HBe,结果显示单克隆抗体(McAb)敏感性高于多克隆抗体(PcAb)。生物素试剂在检测HBeAg的稀释度为1:5000,比酶标法ELISA高1倍多,与RIA相当,且稳定性优于RIA。生物素标记抗体至少1年内保持效价不变,1年内批间变异系数为5.8％,相同标本重复检测变异系数为5.94％,是一种较理想的检测e系统的方法之一。另外,研究发现一孔一期法同时测HBeAg/抗-HBe时,无论标记抗体为单克隆或多克隆 ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】 应用抗PBP2a单克隆抗体,以化学交联法致敏羧化聚苯乙烯乳胶,建立快速检测MRSA-PBP2a的乳胶凝集法?【方法】 以辛酸-硫酸铵法纯化PBP2a单克隆抗体,将纯化后单克隆抗体和羧化乳胶经碳化二亚胺(EDC)共价偶联,并对偶联条件(偶联抗体浓度?乳胶浓度?偶联时间和偶联缓冲液)进行探索和优化,建立检测PBP2a的乳胶凝集法?【结果】 140 mg/L的抗体浓度与15 g/L的羧化聚苯乙烯乳胶,在pH 5.8缓冲液中偶联8 h后有较好的凝集效果和偶联效率,所建立的乳胶凝集法敏感性和特异性良好?【
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Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】 制备均一性和特异性高的抗组氨酰-tRNA 合成酶抗体,为研究皮肌炎和多肌炎的检测提供方法,为制备检测试剂盒提供原料?【方法】 采用常规融合和间接ELISA检测法,获取杂交瘤细胞株;Protein G Sepharose 4FF和Sephadex G50结合对含有单克隆抗体的腹水进行纯化;SDS-PAGA电泳技术进行单克隆抗体的鉴定?【结果】 筛选获得了2株稳定分泌阳性抗体的杂交瘤细胞株,分别命名为W001和W002;杂交瘤细胞克隆后,100%的检测孔保持了分泌抗组氨酰-tRNA合成酶抗体的能力;
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Journal of Diabetes, Volume 15, Issue 12, Page 1081-1094, December 2023., 2023 Highlights Glucagon receptor (GCGR) monoclonal antibody (mAb) improves cardiac function and attenuates microvascular damage in db/db mice. GCGR mAb ameliorates the oxidative stress of cardiac microvascular endothelial cells in diabetic mice. GCGR mAb counteracts the diabetes‐induced upregulation of carnitine palmitoyltransferase 1B, optic atrophy type ...
Peng Wang +6 more
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Veterinary Dermatology, Volume 34, Issue 4, Page 327-338, August 2023., 2023 Background – The caninised monoclonal antibody lokivetmab (LKV), directed at interleukin (IL)‐31, is very effective at controlling pruritus in most dogs with atopic dermatitis (AD). However, evidence exists that IL‐31 is not required for the induction of acute allergic skin inflammation, which might explain why this treatment is less efficacious in ...
Chie Tamamoto‐Mochizuki +4 more
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Veterinary Dermatology, Volume 32, Issue 5, Page 477-e131, October 2021., 2021 Background – Interleukin (IL)‐31 is an important mediator in canine atopic dermatitis (cAD) and also may be dysregulated in other allergic diseases. Hypothesis/Objectives – To demonstrate the efficacy and safety of lokivetmab (canine anti‐IL‐31 monoclonal antibody) for treatment of pruritus associated with allergic dermatitis in dogs.
Leen Van Brussel +4 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 1995 用不实验室建立的亲和层析系统成功地址猪脑分离纯化了苯二氮卓受体（ＢＺ－Ｒ）。结合试验显示，纯化的ＢＺ－Ｒ和专一配基最大的结合容量为１９７０ｆｍｏｌ／ｍｇ蛋白，解离常数Ｋｄ值为５．７ｌｎｍｏｌ／Ｌ，圆盘电泳和等电聚焦电泳均显示出单一区带。纯化受体经ＳＤＳ一聚丙烯酰胺不连续电泳，可分离出分子量分别为５９ｋｕ、５６ｋｕ、５１ｋｕ、４８ｋｕ和３８ｋｕ的５条区带。用这些区带蛋白免疫动物，得到了３种单克隆抗体，酶联免疫检测显示，它们和纯化受体结合的最大滴度分别为１：３００００，１：２２０００，１：２２０００ ...
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Veterinary Dermatology, Volume 31, Issue 6, Page 439-e116, December 2020., 2020 Background It has been demonstrated recently that immunoglobulin (Ig)E specific for cross‐reactive carbohydrate determinants (CCD) is present in the serum of allergen‐sensitized dogs and cats, and that these CCD‐specific antibodies might confound serological testing. Hypothesis/Objective The objective was to document the prevalence of CCD detectable in
Kenneth W. Lee +3 more
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