以ＨＡ２为基础的流感病毒候选疫苗同时与Ｈ５Ｎ１、Ｈ１Ｎ１和Ｈ３Ｎ２亚型毒株产生交叉反应
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘 要： 【目的】 探讨以流感病毒（ＩＡＶ）血凝素蛋白茎部（ＨＡ２）保守表位为免疫原来诱导针对不同血清型流感病毒的广谱体液免疫应答。【方法】 我们构建了一个重组的ＩＡＶ疫苗ＩＲ３０－Ｆｄ，由Ａ／ＶｉｅｔＮａｍ／１２０３／２００４（Ｈ５Ｎ１）病毒株ＨＡ２的３０个保守的氨基酸残基（ＩＲ３０）和一个连在碳端的三聚体基序（ｆｏｌｄｏｎ， Ｆｄ）组成。原核表达该蛋白，圆二色谱和ＷｅｓｔｅｒｎＢｌｏｔ验证ＩＲ－３０－Ｆｄ的室间结构之后，分别免疫ＢＡＬＢ／ｃ小鼠和新西兰白兔，并同时免疫ＩＲ３０多肽为对照，制备血清抗体。
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[CD20-negative/CD10-positive mantle cell lymphoma (blastic variant): a case report]. [PDF]
Zhonghua Xue Ye Xue Za ZhiQiao BB +10 more
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Se PuWei Q, Su FH, Zhu X.
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枸杞多糖通过激活Nrf2/HO-1通路保护HK-2细胞氧化损伤的作用
Zhongguo shiyan zhenduanxue目的 分析枸杞多糖(LBP)干预对氧化损伤的人肾小管上皮细胞(HK-2)内的核因子E2相关因子2(Nrf2)/血红素氧合酶-1(HO-1)通路蛋白表达的变化，探索LBP拮抗HK-2细胞氧化损伤的分子机制。方法 采用过氧化氢诱导HK-2细胞制备氧化损伤细胞模型，HK-2被分成4组：正常组、LBP组、氧化损伤组及LBP干预组。观察4组细胞的长势和形态变化；细胞活力测定法比较每组细胞的存活率；比色法分析氧化产物丙二醛(malondialdehyde, MDA)含量以及超氧化物歧化酶(superoxide ...
赵杰 +6 more
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[Effects and mechanisms of broccoli-derived extracellular vesicles on wound healing of full-thickness skin defects in diabetic mice]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiShen Q, Jian N, Zhang CP, Fu XB.
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[WD40 repeat-containing protein 6 aggravates diabetic kidney disease by promoting lipid accumulation in kidney]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanXin P, Sun L, Niu S, Sun X, Sun X.
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[Effects and mechanisms of cerium-myricetin nanosystem on wound healing in rats with full-thickness burns]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiWang T +7 more
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[Isolation and identification of <i>Pediococcus acidilactici</i> BPA03 derived from human milk and evaluation of its antioxidant and anti-aging probiotic properties]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoCao L, Huang P, Zhang Z, Sun W, Qiu F.
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Hua Xi Kou Qiang Yi Xue Za ZhiDeng Z +7 more
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[Self-designed experiment in instrumental analysis： online solid phase extraction-high performance liquid chromatography- post column derivatization for the determination of trace pesticide residues in vegetables]. [PDF]
Se PuLi Y +8 more
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