Results 131 to 140 of about 953,736 (207)
, 1999 基因靶位操作（ｇｅｎｅｔａｒｇｅｔｉｎｇ）是８０年代发展起来的一项重要分子生物学技术，是通过外源ＤＮＡ与染色体ＤＮＡ间的同源重组，定点修饰、改造基因组特定位点的技术。１同源重组同源重组是基因靶位操作技术的分子生物学基础。ＤＮＡ同源重组在基因转化和遗传..
汪亚平,朱作言
core
Habitat and spatial trends of U.K. wintering waterbird populations over 50 years
Conservation Biology, Volume 40, Issue 5, October 2026.Abstract The United Kingdom is an important wintering ground for millions of waterbirds. Most U.K. wintering waterbird populations increased between 1970 and the mid‐1990s, but declined thereafter. We examined U.K. population indices in 46 wintering waterbird species in two 25‐year periods, 1970–1994 and 1995–2019, to identify which waterbird groups ...
Blaise Martay +8 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2001 【目的】探讨中国人肝豆状核变性(WD)的分子发病机制和基因诊断的方法。【方法】应用基因工程技术对WD 进行了10 年的分子生物学研究。【结果】①WD 的基因定位研究:通过RFLP 及微卫星多态性分析, 应用两位点及多位点连 锁软件, 建立了中国人WD 基因在D13q14 .2 -3区域的精细遗传连锁图谱, 从而首次对中国人WD 基因进行了精确定位;②WD 基因突变研究:应用PCR-SSCP 及DNA 测序技术, 对39 个家系45 名WD 患者进行该致病基因的21 个外显子突变筛选, 发现 WD
徐评议 +4 more
doaj
, 2003 将明恢 63,9311,IR68,献国和BG1639等 5个迟熟水稻品种与早籼核不育系 644 2S 7杂交 ,对F1,F2 代以及三交F1群体进行抽穗期遗传分析 .结果表明 ,9311,IR68,献国和BG1639等 4个迟熟品种含有 1对等位的显性抑制基因 ,可部分地抑制 644 2S 7显性早熟基因的表达 .以 644 2S 7∥明恢 63/ 9311三交F1群体为定位群体 ,利用微卫星标记将该抑制基因定位于水稻第 8染色体短臂上 .该抑制基因命名为Su Ef cd(t) ,并认为该基因对于利用 ...
邓晓建, 杨志荣, 蒋佩琪, 淳泽
core
Risks of concealing environmental degradation
Conservation Biology, Volume 40, Issue 5, October 2026.Abstract Current practice seeks to conceal the visual impact of land‐use change (i.e., development). Six percent of development impact assessments in Australia and 14% of the World Bank's assessments recommend visual impact mitigation. This mitigation results in, for example, vegetated buffer strips alongside cleared agricultural areas and earthen ...
Matt W. Hayward +8 more
wiley +1 more source
, 2013 中药材及中成药的真实性是中药质量控制的关键环节，现有以化合物为检测目标的检测方法存在很多弊端，本文利用近代分子生物学发展的最新成果，首次将聚合酶链式反应（PCR）与新型核酸探针相结合应用于中成药的基因检，主要包括以下几项工作： 1. 建立了含有人参、党参药材的中成药的基因检测方法，首次在经过高度工业加工的口服液和注射液中检测到了药材的DNA；重点对液体制剂的DNA提取方法进行了研究与优化；最后利用G-四聚体-杂交链式反应（GQ-HCR）将该方法发展成一种可视化芯片的检测模式 ...
陈蓉
core
Scientists’ warning on the global destruction of rock outcrop ecosystems
Conservation Biology, Volume 40, Issue 5, October 2026.Abstract Rock outcrops are geological formations that harbor a highly specialized biota adapted to harsh environmental conditions that differ from their surrounding landscapes. They are globally distributed, especially in old, highly weathered landscapes, and can function as habitat islands containing high levels of endemism and distinct evolutionary ...
Luiza F. A. de Paula +19 more
wiley +1 more source
野生动物学报, 2017 经PCR扩增和序列测定,获得了无蹼壁虎(Gekko swinhonis)的线粒体基因组全序列,其总长为16 818 bp,含有13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个控制区。各基因的位置、排列顺序及转录方向均和大部分的有鳞目物种一致。无蹼壁虎线粒体基因组的碱基组成分别为:A(31.35%)、T(26.28%)、C(27.71%)、G(14.67%)。除Ser(GCU)缺失DHU臂外,其余的21个tRNA都可以形成典型三叶草结构。13个蛋白质编码基因中 ...
秦 峰 曾德龙 高成伟 秦新民
doaj
Conservation Biology, Volume 40, Issue 5, October 2026.Abstract Ecological niche models (ENMs) are used to assess the abiotic preferences of species by linking their occurrences to the environmental conditions in which they live. We developed a fossil‐informed ENM framework that integrates mid‐Holocene and modern occurrences to test niche stability and reconstruct abiotic niche characteristics for four ...
Claire. M. Williams +3 more
wiley +1 more source
四川大学学报. 自然科学版, 2002 以质粒 pBUSCK HPT ,pCUSBK HPT和pGNA HPT作抗虫基因供体 ,优良籼型三系恢复系SH5 2 7和广亲合系W 2 0 0 8为受体 ,采用基因枪转化法 ,获得了转修饰的豇豆胰蛋白酶抑制剂基因 (sck)、修饰的Bt毒蛋白基因 (sbk)和雪花莲凝集素基因 (gna)的植株 .通过人工杂交 ,将外源基因聚合 .分子证据显示 ,作者获得了抗性基因聚合的材料 .蛋白活性测定表明 ,聚合基因的表达因载体结构相似而受到影响 ,但抗虫性却得到提高 ...
马炳田 +4 more
doaj