Results 131 to 140 of about 62,299 (213)
糖尿病、冠心病患者ABCA1基因R219K多态性的频率分布特点
Zhongguo shiyan zhenduanxue, 2011 目的旨在探讨我国北方地区汉族人ABCA1基因R219K多态性的频率分布特点,对血脂水平的影响,及其与冠心病、糖尿病和糖尿病合并冠心病的关系。方法采用改良碘化钠法提取基因组DNA,聚合酶链反应限制性片段长度多态性（PCR-RFLP）法分析ABCA1基因R219K多态性。结果正常对照组ABCA1基因R219K多态性RR、RK、KK基因型频率、R等位基因频率、K等位基因频率分别为28.5%、48.6%、22.9%、52.8%、47.2%;糖尿病组分别为29.6%、63.9%、6.5%、61.5%、38.5 ...
许慧, 彭浩, 蒋莹
doaj
Emotion and the Advocacy Coalition Framework: An Affective Dynamics Perspective
Policy Studies Journal, Volume 54, Issue 3, August 2026.ABSTRACT Despite extensive evidence that emotion and cognition are deeply intertwined, the Advocacy Coalition Framework (ACF) lacks an analytically independent emotional mechanism in its causal architecture—an omission that may be particularly consequential in policy subsystems structured around morally charged, identity‐laden policy disputes.
Moshe Maor
wiley +1 more source
, 2001 本文综述了细菌人工染色体 (BacterialArtificialChromsomes ,BAC)的构建、物理图谱制作方法及其靶位精细操作策略的研究进展。同时 ...
胡炜,朱作言
core
, 2013 基于四膜虫小核rDNA改造得到的pD5H8载体，在转染进接合生殖时期的四膜虫后能发育成9000～10000拷贝的大核rDNA，因此pD5H8载体是四膜虫异源基因表达的研究热点；但其载体上匮乏合适的克隆位点和无启动子结构阻碍了该载体的进一步发展。本研究在四膜虫启动子HSP70—2和终止子HSP70-I之间，通过稀有酶切位点I-SceI导入细菌绿色荧光蛋白NGFP）这一筛选标记，由此组成的DNA片段（HSP70-25’UTR．I-SceI—HGFP．I—SeeI—HSP70-13’UTR）整合进pD5H8 ...
冯立芳, 熊杰, 袁冬霞, 缪炜
core +1 more source
OPN基因启动区-156delG多态性与中国HBV相关肝细胞癌的易感性
Zhongshan Daxue xuebao. Yixue kexue ban, 2017 【目的】研究骨桥蛋白（OPN）基因启动区多态性与乙型肝炎病毒相关肝细胞癌（HBV-HCC）遗传易感性的关 系。【方法】225例确诊为HBV-HCC患者与200例年龄匹配的慢性乙型肝炎患者被纳入研究。OPN基因启动区3个多态性 位点（-156delG/G ，-443T/C和-616T/G）通过直接测序法确定基因型。【结果】HBV-HCC组-156 delG/delG基因型分布频率高 于对照组（P = 0.003）。HCC组-156 delG等位基因频率显著增高（P < 0.001 ...
时红, 吴元凯, 林国莉, 李向永
doaj
Justice in coexistence: Pastoralism and large carnivores on the Qinghai‐Tibet Plateau
People and Nature, Volume 8, Issue 7, Page 2186-2199, July 2026.Abstract The conflict between livestock husbandry and large carnivore conservation presents significant challenges in grassland ecosystems worldwide. Achieving sustainable coexistence among herders, livestock and large carnivores requires reconciling diverse perspectives and interests through equitable, inclusive and fair processes that address both ...
Yufang Gao, Yue Yu
wiley +1 more source
, 2007 本发明公开了一种不育转基因鱼的制备方法，以鲤鱼肌动蛋白基因启动子作为启动子，以反义的２８６ｂｐ鲤鱼鲑鱼型促性腺激素释放激素基因互补脱氧核糖核酸片段［包括ｓＧｎＲＨ十肽、促性腺激素释放激素相关肽编码区和６４ｂｐ的３′非编码区序列］为靶位基因，草鱼生长激素基因３′侧翼序列为终止顺序，构建鲑鱼型促性腺激素释放激素反义ＲＮＡ表达载体，采用显微注射法将重组ＤＮＡ片段导入鲤鱼受精卵，聚合链式酶反应及放射免疫检测筛选获得不育转基因鱼。本发明方法易行，操作方便 ...
汪亚平, 朱作言, 胡炜, 黎双飞
core
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 观察24个常用STR基因座等位基因突变的现象及特点65377; 【方法】 对5 084例肯定亲权关系的案例进行分析,筛选等位基因突变事件,确定突变等位基因的来源,统计各STR基因座的突变率,分析突变的规律及其特点65377; 【结果】 在24个STR基因座中共观察到172个突变事件,STR基因座的突变率介于0 ~ 0.492%65377;每个突变案例的突变基因座数目为1 ~ 3个65377;突变模式符合逐步突变模式,最多突变步数为四步65377;父系突变与母系突变比例为3.55:165377;
doaj
青岛地区大肠埃希菌临床株中blaCTX-M和PMQR基因的检测
Zhongguo shiyan zhenduanxue, 2014 目的调查青岛地区大肠埃希菌临床分离株中β-内酰胺酶基因blaCTX-M和质粒介导的喹诺酮耐药（Plasmid-mediated quinolone resistance,PMQR）基因的流行性。方法采用Vitek-2compact系统进行菌株鉴定和药物敏感性测定。聚合酶链反应（Polymerase chain reaction,PCR）法检测菌株中存在的blaCTX-M基因和各种PMQR基因,并对PCR扩增产物进行DNA序列测定,确定基因类型。结果 83%的大肠埃希菌对头孢菌素类和氟喹诺酮类药物耐药 ...
尉鸿飞 +5 more
doaj
Visible‐Light‐Excited Optical Thermometry in Ca3Sc2Si3O12: Er3+ via an Efficient Eu2+ Bridge
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Er3+ ions are promising for optical thermometry due to their thermally coupled levels. However, their parity‐forbidden transitions result in weak and narrowband absorption, which restricts excitation to infrared lasers (e.g., 808, 980, or 1550 nm). Moreover, the single emission center inherently limits temperature sensitivity. In this work, we
Yi Liu +6 more
wiley +1 more source