Results 141 to 150 of about 953,736 (207)

全长基因的克隆

open access: yes, 2005
新基因全长cDNA序列、全长DNA序列的获得常常是分子生物学工作者面临的难题。本文就克隆全长基因的各种技术如文库筛选法 ...
王闵霞, 谭红, 代富英, 马欣荣
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Psychometric framework for coexistence with large carnivores

open access: yesConservation Biology, Volume 40, Issue 5, October 2026.
Abstract Human–carnivore coexistence is essential for biodiversity conservation, yet measuring the attitudes and behaviors that support it remains methodologically challenging. We developed psychometric scales to assess willingness to coexist with carnivores and the underlying beliefs of this coexistence through an iterative expert elicitation process ...
Benjamin Ghasemi   +14 more
wiley   +1 more source

The proposed protocol for the detection system of transgenic plants in Taiwan

open access: yes, 2011
基因轉殖植物之檢測有兩種方式,第一種是藉由基因表現的層次進行觀察檢測,如:基因表現蛋白質或植物之外在表徵等;第二種方式係就DNA 層次直接檢測轉殖之外來基因的DNA 序列。就基因表現層次方面而言,目前台南區農業改良場利用除草劑進行耐藥性篩檢,供試的21 個玉米品種均對嘉磷塞(Glyphosate)(年年春41%S、80 倍稀釋液噴施)及固殺草(Glufosinate)(百試達18.02%S、100 倍稀釋液噴施)無耐藥性。僅進口飼料玉米具有耐藥性之個體。花蓮區農業改良場則已發展以x-gluc ...
Ming-Jen Fan; Shu Chen; Chien-Yih Lin; Chi-Chu Tsai; Ai-Hua Yang; Shyh-Shyan Wang; Hsueh-Shih Lin   +1 more
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Behavioral interventions to reduce demand for threatened freshwater turtles as pets

open access: yesConservation Biology, Volume 40, Issue 5, October 2026.
Abstract Growing demand for freshwater turtles as pets has fueled illegal trade and accelerated wild population declines, underscoring the need for theory‐based demand‐reduction interventions. We conducted a three‐part randomized controlled trial with active turtle keepers (n = 1800) in China to test conservation‐ and legality‐framed messages by ...
Wuji Zheng   +9 more
wiley   +1 more source

一种用于研究转基因和非转基因水稻代谢差异的方法

open access: yes, 2011
本发明公开了一种水稻代谢轮廓分析及代谢差异研究的方法,采用液相色谱-质谱联用技术对水稻种子提取物进行分析得到水稻代谢轮廓谱,然后用多变量统计方法研究转基因水稻和非转基因水稻的代谢轮廓数据的整体差异,并发现对代谢表型差异有重要贡献的化合物,为转基因水稻的非预期效应和安全性评价提供依据。本发明的分析方法简单,快速,重复性好,适合于实际样品的批量分析 ...
赵春霞   +5 more
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Predictive evolutionary genomics: principles, validation, and practice

open access: yesNew Phytologist, Volume 252, Issue 1, Page 36-48, October 2026.
Summary Climate change and habitat loss are driving rapid evolutionary responses in populations world‐wide, which creates an urgent need for evolutionary forecasting in conservation and agriculture. Such forecasting can be categorized into three time scales: trait‐based models that use multivariate quantitative genetic equations to project correlated ...
Daniel Ortiz‐Barrientos   +5 more
wiley   +1 more source

不育转基因鱼的制备方法

open access: yes, 2007
本发明公开了一种不育转基因鱼的制备方法,以鲤鱼肌动蛋白基因启动子作为启动子,以反义的286bp鲤鱼鲑鱼型促性腺激素释放激素基因互补脱氧核糖核酸片段[包括sGnRH十肽、促性腺激素释放激素相关肽编码区和64bp的3′非编码区序列]为靶位基因,草鱼生长激素基因3′侧翼序列为终止顺序,构建鲑鱼型促性腺激素释放激素反义RNA表达载体,采用显微注射法将重组DNA片段导入鲤鱼受精卵,聚合链式酶反应及放射免疫检测筛选获得不育转基因鱼。本发明方法易行,操作方便 ...
汪亚平, 朱作言, 胡炜, 黎双飞
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