Results 51 to 60 of about 7,319 (164)
外泌体环状RNA诱导HK-2细胞凋亡在感染性休克AKI中的研究
Zhongguo shiyan zhenduanxue目的 探究外泌体环状RNA诱导HK-2细胞凋亡在感染性休克相关性急性肾损伤的作用机制。方法 分为感染性休克相关性急性肾损伤患者组和健康人对照组，采集血标本。检测外泌体标记蛋白，鉴定出两组外泌体，对外泌体环状RNA进行实时PCR分析。对两组的HK-2细胞进行凋亡检测。结果 10例感染性休克AKI入院患者，10例健康人对照。鉴定表面外泌体标记蛋白包括HSP70和TSG101。两组均鉴定出外泌体。与对照组相比，感染性休克AKI组circ-Ttc3 ...
李秀君, 李岩, 张春媚, 杨海玲
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Zhongguo shiyan zhenduanxue, 2019 外泌体最早由Johnstone等在研究网织红细胞成熟过程中囊泡的形成时发现,是一种由各种活细胞通过内体途径产生并分泌的直径在40-100nm级的小囊泡,由脂质双分子层包裹[1]。在外泌体被发现的最初十几年中,它的生物学作用一直没有引起人们足够的重视,直到1996年,有科学家发现B淋巴细胞能够分泌具有抗原提呈作用的外泌体,这种外泌体携带有MHC-II类分子、共刺激分子和黏附分子,能够直接刺激效应CD4+T细胞进而产生抗肿瘤效应[2]。随后 ...
张传霞, 崔雅楠, 金浩范
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的探究环状RNA hsa_circ_0002316（circOMA1）是否参与泌乳素瘤耐药调控及其作用机制是什么。方法通过RT-qPCR检测临床泌乳素瘤标本中circOMA1表达情况（5例敏感泌乳素瘤标本和12例耐药泌乳素瘤标本）。通过慢病毒载体构建稳定表达外源性circOMA1 MMQ细胞系模型进行体外实验，分为MMQ NC组及MMQ OMA1组。通过构建裸鼠皮下肿瘤模型进行体内实验，分为MMQ NC+卡麦角林（CAB）组、MMQ NC+溴隐亭（BRC）组、MMQ OMA1+CAB组、MMQ ...
张译尹 +5 more
doaj
Ginseng-derived Nanoparticles Inhibit Lung Cancer Cell Growth by Promoting Macrophage M1 Polarization [PDF]
Objective: To explore the molecular mechanism by which ginseng-derived nanoparticles (GDNPs) inhibit the growth of human non-small cell lung cancer cells (A549) by altering the polarization state of macrophages.
Daqing ZHAO +7 more
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Progress in the role of exosomes in sepsis⁃induced cardiomyopathy [PDF]
"Sepsis is a life⁃ threatening organ dysfunction triggered by a dysregulated host response to infection. Sepsis⁃ induced cardiomyopathy（SICM）is an acute cardiac condition caused by sepsis，characterized by high incidence and extremely high mortality ...
ZHENG Ye*，LI Shaoling，HAN Fei
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A study on communication mechanism of lung cancer cells in tumor microenvironment mediated by pleckstrin-2/miR-196a signal axis [PDF]
Background and purpose: It is still a great challenge to clarify the signal molecules that mediate the communication between cancer-associated fibroblasts (CAFs) and tumor cells. These signal molecules are very important for cancer metastasis.
WANG Manli, CHEN Hui, DUAN Zhi, XU Qimei, LI Zhen
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Zhongguo shiyan zhenduanxue, 2019 外泌体是由脂质双层膜包裹的,含有蛋白质、多肽和核酸的纳米级细胞外囊泡。目前已经从外泌体中鉴定出4563余种蛋白质、194余种脂类、1639余种mRNA、764余种miRNA等非编码RNA (lncRNAs)以及mtDNA、ssDNA和dsDNA等[1,2]。外泌体含有的多种功能性核酸及蛋白质,在细胞物质转运和信息传递过程中发挥重要作用,影响着细胞的生长与分化。近年来研究表明,lncRNA了参与细胞增殖、分化、染色体重塑、表观遗传调控、转录和转录后修饰等多种生物学过程,也引起大家越来越多的关注 ...
薛婷, 姜义, 史莹, 张颖超
doaj
, 2018 环状RNA(circular RNA,circ RNA),作为非编码RNA(non-coding RNA)的重要成员,广泛存在于包括人类在内的多种原核及真核生物体内,对生命活动具有重要的调控作用,已成为非编码RNA研究领域的热点。与线性RNA相比,circ RNA呈共价闭合环状结构,具有结构稳定、序列保守、组织特异性、时空特异性等特点,在包括消化道肿瘤在内的多种疾病中发挥重要作用。circ RNA可通过竞争性吸附mi RNA、调控基因表达、参与蛋白质翻译等多种途径,参与肿瘤的发生发展,在肿瘤早期诊断 ...
施颖, 李金莹, 黄卫
core
Progress in the treatment of sepsis⁃associated acute kidney injury [PDF]
"Sepsis⁃associated acute kidney injury（SA⁃AKI）is a common critical illness in hospitalized patients，characterized by high incidence，high mortality. The pathogenesis of SA ⁃ AKI is complex，with both direct mechanisms related to infection and indirect ...
LIANG Xinyue*，ZHOU Yun
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Research progress of plant exosome-like nanoparticles on characteristics, components and functions [PDF]
Exosome-like nanoparticles （ELNs） are nano-sized extracellular vesicles enclosed by lipid bilayer membrane and secreted by cells; And carrying a variety of cargo, including lipids, proteins, miRNAs and secondary metabolites.
CHEN Chunping +4 more
core +1 more source