Results 91 to 100 of about 24,580 (225)
Molecular Cytogenetic Studies on Haliotis diversicolor diversicolor, H. discus hannai and the interspecific hybrid [PDF]
, 2016 近年来鲍类养殖存活率急剧下降，种质退化和大规模疾病的发生成为重要原因。为此，开展基础研究迫在眉睫，对遗传背景的研究已成为解决实践问题的前提。本研究以杂色鲍(Haliotisdiversicolordiversicolor)与皱纹盘鲍(H.discushannai)及其杂交子代为实验材料，应用荧光原位杂交（Fluorescenceinsituhybridization，FISH）技术和基因组原位杂交（Genomeinsituhybridization，GISH）技术对它们的染色体进行研究 ...
刘圆圆
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Geographic risks to functional groups of mammals and birds from habitat loss in Mexico
Conservation Biology, EarlyView.Abstract There is a need to quantify the impact of habitat loss due to anthropogenic factors on different aspects of biodiversity, such as functional trait diversity represented by functional groups (FGs). We developed a metric to assess the weighted risk of loss of habitat for 33 FGs of mammals and 36 FGs of birds in Mexico based on potential ...
Fernando Mayani‐Parás +4 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2007 目的采用PCR-CTPP技术,检测中国浙南地区白细胞介素-2（IL-2）基因-330位的多态性,并分析其可能的临床应用。方法针对IL-2基因-330（T/G）多态性设计两条序列特异性引物,结合两侧引物,在同一反应管中进行PCR。对PCR条件进行优化,并与测序结果进行对比。在此优化条件对101名体检个体的DNA进行IL-2基因多态性分析。结果通过条件优化,PCR-CTPP（polymerase chain reaction with confronting two-pair primers）可以区分IL ...
杨丽红 +4 more
doaj
Gaoya dianqi, 2021 相变冷却技术逐渐成为高压功率电力电子器件散热瓶颈的解决方案。但在高压应用场景中,由气泡引发的绝缘问题需要重点关注。针对相变冷却的沸腾特征,掌握制冷剂液态、气态以及两相态的绝缘特性是开展相变冷却技术绝缘分析的基础,而现有研究尚没有系统性的制冷剂多相态绝缘特性测试方法。因此,文中研制了用于制冷剂多种相态工况的绝缘实验装置,首次实现了低沸点液体蒸汽的介电强度测试,进而提出了制冷剂蒸汽及两相态环境的击穿实验方案。利用FC-72制冷剂,对其液态、气态及两相态展开了系统性的击穿特性研究,获得了FC ...
莫申扬 +5 more
doaj
[Recent research on the association between depressive disorder and gene polymorphisms in adolescents]. [PDF]
Zhongguo Dang Dai Er Ke Za Zhi, 2023 Yao KK, Dong QL, Ye LX.
europepmc +1 more source
Creativity Screening of Product Innovation Based on Meta-synthesis Methodology [PDF]
, 2016 科学技术的迅猛发展、网络化以及经济全球化时代的到来，使得产品创新成为企业在激烈的国际环境中竞争的重要手段。从企业生产经营的流程来看，相对于生产和营销阶段，新产品开发中从创意产生到具体的创新方案提出的产品创新阶段，还没有实现科学化、工程化的管理和信息系统的支持。因此，单独对此阶段进行研究十分必要。本文将产品创新分为了产品需求、产品创意、产品设计、产品论证和产品评估阶段。并提出在对各阶段的复杂性进行分析的基础上，利用综合集成方法理论，根据各阶段需求和目标的不同，有针对性地提出解决方法 ...
廖容
core
Conservation Biology, EarlyView.Abstract There exists a growing suite of technologies that support significant and exciting progress in biodiversity conservation and research. Citizen scientist participation is common in this research and often focuses on data collection and labeling.
Joycelyn Longdon +5 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 1998 调查上海部分地区正常人血清ａｐｏＥ浓度，了解它与年龄和性别的关系；建立ａｐｏＥ基因型的检测方法。方法采用夹心ＥＬＩＳＡ法进行ａｐｏＥ定量，应用多聚酶链反应（ＰｏｌｙｍｅｎａｓｅｃｈａｉｎｒｅａｃｔｉｏｎＰＣＲ）和限制性片段长度多态性分析（ＲｅｓｔｒｉａｔｉｏｎｆｒａｇｍｅｕｔｌｅｎｇｔｈＰａｌｙｍｏｒｐｈｉｓｍ，ＲＦＬＰ）建立ａｐｏＥ多态性分析法。结果２００份正常人血清ａｐｏＥ定量确立参考值范围为８０±５３ｍｇ／Ｌ，女性略高于男性，不同年龄无显著差异；建立了快速抽提ＤＮＡ方法和ＰＣＲ扩增及ＲＦＬＰ法 ...
沈霞 +3 more
doaj
The effect of pentosan polysulfate on diabetic nephropathy renal tubular epithelial cells injury through regulating p38 MAPK signaling pathway [PDF]
, 2016 目的：观察戊糖多硫酸钠是否通过调控P38MAPK干预糖尿病肾病肾小管上皮细胞的损伤。方法：利用不同浓度（10mmol/L～50mmol/L）葡萄糖刺激肾小管上皮细胞（HK-2）48h，应用CCK-8比色法检测肾小管上皮细胞增殖的改变。利用不同时间段（0h～48h）葡萄糖刺激细胞，用Westernblot法检测P38MAPK蛋白的表达水平。分为正常对照组（control组）、高糖组（HG组，葡萄糖30mmol/L)、戊糖多硫酸钠（PPS）组（PPS200µg/mL)、高糖＋戊糖多硫酸钠组（HG＋
张天英
core
Conservation Biology, EarlyView.Abstract Human development is a driver of global change and a major threat to biodiversity. Protected areas maintain and support biodiversity, but outside stressors, such as climate change and land use change, can negatively influence natural resources within protected areas.
Leah J. Rudge +2 more
wiley +1 more source