Results 91 to 100 of about 6,735 (193)
Analysis of transcriptome and chromatin accessibility changes during the differentiation of human embryonic stem cells into neural progenitor cells [PDF]
Objective·To investigate the changes in transcriptome and chromatin accessibility during the differentiation of human embryonic stem cells (hESCs) into neural progenitor cells (NPCs) using in vitro differentiation models and high-throughput multi-omics ...
CAI Xiaodong +9 more
core +1 more source
Zhongguo shiyan zhenduanxue目的利用公开的帕金森病(Parkinson’s disease，PD)外周血的基因表达谱数据，通过免疫浸润分析其免疫差异，利用差异基因分析、构建蛋白-蛋白相互作用（Protein-protein interaction，PPI）网络，寻找PD新的潜在生物标志物，为探究PD的相关免疫特征提供新的思路。方法本研究所用的基因转录组RNA测序数据（GSE6613和GSE57475)和患者临床性状相关数据均来源于GEO数据库。外周血转录组数据包括50例PD、93例早期帕金森病（Early Parkinson’s ...
何敏 +6 more
doaj
Shuitu Baochi Xuebao, 2011 采用野外调查采样和室内培养、分析相结合的方法,探讨蔬菜对多氯代有机污染物(PCOPs)的富集和转移特征。结果表明:不同旱地蔬菜对不同PCOPs的富集能力和转移能力各不相同,主要体现在叶菜类对六六六(HCB)的富集系数最高,根菜类对滴滴涕(DDT)类的转移系数最高等特点;不同盆栽蔬菜的富集和转移特点和旱地蔬菜的基本一致,且随着土壤受污染程度的加剧会导致其富集系数和转移系数降低,这与蔬菜生物量较小而影响其对PCOPs的富集和转移能力有关 ...
万大娟, 贾晓珊, 谭长银
doaj
Study on the effect and mechanism of sorting nexin 1 on inhibiting the proliferation and migration of colorectal cancer cells [PDF]
Objective·To explore the expression of sorting nexin 1 (SNX1) in colorectal cancer (CRC) and its impact on the proliferation and migration of CRC cells.Methods·Transcriptomic data and clinical pathological information of CRC were obtained from The Cancer
LI Shuxin +4 more
core +1 more source
基于生物信息学途径评估 UBE2 家族及 UBE2T 基因在肺腺癌中的表达及临床意义 [PDF]
, 2020 【目的】探究泛素结合酶E2家族和泛素结合酶E2T（UBE2T）在肺腺癌中的表达情况及其与临床病理特征之间的关系。【方法】利用linkedomics平台进行UBE2C、UBE2M、UBE2S、UBE2T、UBE2V2 mRNA表达水平与肺腺癌临床特征之间的关系分析，对总生存时间（OS）（Cox回归检验）、总分期（Kruskal-WallisH检验）、T分期（Kruskal-WallisH检验）、N分期（Kruskal-WallisH检验）及M分期（Wilcox秩和检验）进行差异比较。运用肿瘤基因组图谱 ...
杨豪帅 +3 more
core
Dianci bileiqi, 2017 为研究山东电网绝缘子表面污秽成分的特点,选取不同运行环境、不同电压等级和不同电场的绝缘子,采用离子色谱等方法分析污秽中的主要成分。研究结果表明:自然降水的冲刷效果,造成绝缘子表面难溶性盐CaSO4的富集特性比易溶性盐显著;重污区绝缘子污秽中主要成分含量明显高于其他环境;相同运行环境,500 k V绝缘子污秽中主要离子成分的含量明显高于220 k V、110 k V绝缘子;直流电场对污秽的吸附能力比交流电场强,但对污秽成分的影响不大。采用富集因子分析方法,分析了污秽中主要金属元素Ca的来源 ...
刘嵘 +7 more
doaj
Effect of STX5 on the metastasis of hepatocellular carcinoma and its mechanism [PDF]
Objective To investigate the effect of STX5 on the metastasis of hepatocellular carcinoma (HCC) and its mechanism. Methods Clinical data were collected from 36 patients who were diagnosed with HCC in our hospital from January to December 2015, and the ...
GUO Tingting, ZHAO Ziyin, HE Mingyang, WU Tiansong, XU Bin, ZHANG Bin, WU Zehua, HAN Bing
core +1 more source
Effect of ghrelin gene knockout on the postsynaptic potential of dopaminergic neurons in the substantia nigra of mice [PDF]
Objective To study the effect of ghrelin gene knockout on the postsynaptic potential of dopaminergic neurons in the substantia nigra of mice. Methods Substantia nigra tissue was taken from 10-week-old ghrelin-/- male mice (ghrelin-/- group) and their ...
LIU Jing, LI Huanhuan, JIAO Qian, CHEN Xi, JIANG Hong, DU Xixun
core +1 more source
Zhongguo shiyan zhenduanxue目的基于4D Lable-free蛋白质组学技术分析富氢水干预对老年性聋小鼠耳蜗差异蛋白表达的影响，探索其作用机制。方法20只雄性C57BL/6J小鼠随机分为5组（n=4）：年轻组、自然衰老组、诱导衰老组、自然衰老富氢水治疗组、诱导衰老富氢水治疗组。检测5组实验小鼠的听觉脑干反应（ABR）听力阈值，取耳蜗组织进行蛋白组学分析，包括差异蛋白筛选、基因本体（GO）、京都基因与基因组百科全书（KEGG）富集分析。结果共鉴定到3 925个差异表达蛋白。年轻组与自然衰老组差异表达蛋白共有559个（上调蛋白400个，
于书剑, 汪欣, 于红
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】采用生物信息学方法探究肝细胞癌（HCC）发生的关键基因，望有助于了解HCC发生发展的分子机制。【方法】从GEO数据库中选择基因表达谱GSE76427，该数据集包含115例肝癌组织样本和52例肝癌临近非肿瘤组织样本。通过GEO2R工具在线分析，筛选出肝癌组织中差异表达基因（DEG），利用GO数据库获取DEG的功能注释，利用KEGG数据库进行通路富集分析。基于STRING数据库，利用MCC算法，筛选具有高度连接性的关键基因,基于TCGA数据库在线软件分析关键基因的预后效应。【结果 ...
曾洁, 邓伟, 黄天壬
doaj