Label-Free Quantitative Comparison of N-Glycoproteomics between Camel and Cow Milk [PDF]
To understand the differential N-glycoprotein characteristics of camel versus cow milk, we employed label-free quantitative N-glycoproteomics to compare the N-glycoprotein composition and the number of N-glycosylation sites between the two milks and ...
LI Danlei, KONG Xiabing, YU Yue, HU Qian, ZHANG Jiukai, CHEN Ying
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构建MS2-RIP方法用于鉴定lncRNA DANCR在肿瘤细胞中的相互作用分子
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的为了探讨lncRNA DANCR在肿瘤细胞中的作用机制，本研究拟构建一种新的MS2-RIP方法用于鉴定DANCR的相互作用分子。方法利用MS2噬菌体衣壳蛋白可与噬菌体复制酶编码基因5’端一段由19个碱基组成的RNA茎环结构序列，即MS2结合位点（MS2 Binding Site，MS2 BS）高效结合的特点，构建串联表达MS2 BS和DANCR的过量表达质粒，通过MS2蛋白与MS2 BS的亲和作用，使DANCR得到富集，并且同步捕获与DANCR相互作用的分子，用于进一步分析鉴定 ...
巫孟师 +4 more
doaj
Screening of endoplasmic reticulum stress characteristic genes and immune infiltration manifestations in chronic obstructive pulmonary disease [PDF]
Objective To identify the core genes of endoplasmic reticulum stress (ERS) in chronic obstructive pulmonary disease (COPD) using the bioinformatics, various machine learning algorithms and experimental validation.
HE Zhiyi, LUO Chenyang, ZHANG Shuang
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Exploring Antibacterial Effect and Mechanism of Flavonoids in Ampelopsis Based on Network Pharmacology, Molecular Docking and Experimental Verification [PDF]
To investigate the antibacterial effect and mechanism of flavonoids in Ampelopsis, network pharmacology was used to select key antibacterial flavonoid components in Ampelopsis and core targets.
ZHANG Lihui, WANG Dandan, MENG Yanlin, WANG Meihui, ZHU Xiaoyong, YAN Zhiqiang, HE Li, HUANG Wei, WANG Qiaoyan
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基于转录组数据分析M2巨噬细胞在胶质瘤免疫微环境中的分子特征
Zhongguo shiyan zhenduanxue目的基于CGGA及TCGA数据库中的转录组数据，分析M2巨噬细胞在胶质瘤免疫微环境中的分子特征，为在胶质瘤中的免疫治疗提供新思路。方法通过“xCELL”R包计算每位患者免疫浸润细胞得分，并分析M2巨噬细胞与临床特征间的联系。利用Kaplan-Meier、ROC曲线及COX回归分析评估M2型巨噬细胞作为临床预后指标的潜力。此外，应用基因本体论（GO）、京都基因与基因组百科全书（KEGG）及基因集富集分析（GSEA）来揭示M2型巨噬细胞在肿瘤免疫微环境中的功能。最后 ...
黄卓彦 +3 more
doaj
Shuitu Baochi Xuebao, 2004 对紫色土坡地6种种植模式下的径流泥沙与养分流失进行了观测统计分析,结果表明,6种种植模式下的径流量、泥沙流失量及养分流失量存在显著的差异,南方早熟梨 紫花苜蓿及柑橘 鸭茅两种果草模式与其它种植模式相比其径流过程曲线较为平滑,无突显的径流峰值点,径流量最少,两种果草模式各项养分流失量与传统顺坡种植模式相比减少了4～7倍不等;流失泥沙中的养份含量高于背景土壤和溶蚀携带,具有富集养分的特征,其富集比大多在1.3～2.4之间。
蒋光毅 史东梅 卢喜平 刘玉民
doaj
Exploring the mechanism of platycodin D in inhibiting nasopharyngeal carcinoma based on network pharmacology combined with experimental validation [PDF]
Objective To delve into the underlying mechanism of platycodin D in inhibiting nasopharyngeal carcinoma (NPC) through a combination of network pharmacology and cellular experimental techniques.
CHEN Yanfang +7 more
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Exploring the Mechanism of Hippophae Fructus Anti-obesity through Network Pharmacology and Molecular Docking [PDF]
Objective: To investigate the active components, protein targets, and mechanisms underlying the anti-obesity effects of Hippophae fructus using network pharmacology and molecular docking techniques, and to validate its in vitro anti-obesity efficacy ...
Chun ZHANG +8 more
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Proteomics and Phosphoproteomics Analysis of Effect of Retinoic Acid-Induced Protein 16 Knockout on Human Colon Cancer Cells [PDF]
ObjectiveTo analyze the differences in the expressions of the total and phosphorylated proteins in human colon cancer HCT116 cells after the knockout (KO) of retinoic acid-induced protein 16 (RAI16) and explore the possible mechanism and related ...
Cuiling DING +4 more
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