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体外培育牛黄通过调控小胶质细胞极化及炎症小体活性干预脑缺血再灌注损伤 [PDF]
浙江大学学报. 医学版目的：探讨体外培育牛黄（ICCB）减轻脑缺血再灌注损伤（CIRI）的机制。方法：分别构建大脑中动脉阻塞（MCAO）大鼠模型和氧糖剥夺/再灌注（OGD/R）BV2细胞模型。其中，大鼠模型于脑缺血1.5 h再灌注72 h后进行改良的神经损伤严重程度评分（mNSS），并测定脑组织含水量和脑梗死体积；苏木精-伊红染色法检测大脑皮层及海马CA1区组织结构病理变化；免疫荧光法检测皮层小胶质细胞极化情况和NOD样受体热蛋白结构域相关蛋白（NLRP）3炎症小体的表达情况。BV2细胞于氧糖剥夺0.5 h再灌注24 h后 ...
褚 坦路 +7 more
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Journal of Diabetes, Volume 15, Issue 10, Page 866-880, October 2023., 2023 Highlights Nattokinase rescued endothelial dysfunction and pericyte loss in the diabetic retina. Nattokinase attenuated diabetes‐induced retinal inflammation and neurodegeneration. High mobility group box 1 signaling was involved in nattokinase‐mediated retinal protection.
Zijing Huang +11 more
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Zhongguo cuzhong zazhi, 2023 脑出血是一种由脑实质血管破裂引起的严重疾病，目前尚无有效的治疗方法。研究表明炎症反应在脑出血继发性脑损伤中发挥了重要作用。在出血部位，小胶质细胞被迅速招募并激活，且在促炎型与抑炎型之间动态转变，不同的信号通路可以改变小胶质细胞极化的状态，从而对脑损伤起到调控作用，这提示了小胶质细胞在脑出血继发脑损伤的病理过程中具有复杂的作用机制。促炎型小胶质细胞释放的各种炎症因子介导神经细胞和神经组织的炎症损伤；抑炎型小胶质细胞释放趋化因子、脑源性神经营养因子、抗炎介质等诱导小胶质细胞向病变方向迁移 ...
孔德敏1，邹伟2
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Zhongguo shiyan zhenduanxue, 2023 目的 探讨氯化两面针碱(nitidine chloride, NC)对胶质瘤LN18细胞增殖、迁移和侵袭能力的影响。方法 以胶质瘤LN18细胞为研究对象。实验分为：对照组和NC处理组。采用CCK-8法检测胶质瘤细胞的增殖情况；用划痕和Transwell小室实验检测胶质瘤细胞的迁移和侵袭能力。结果 与对照组相比，NC处理组胶质瘤细胞增殖能力与对照组有显著差异 ...
朱长吉 +4 more
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Zhongguo cuzhong zazhi, 2022 目的 探讨小鼠脑缺血损伤后阿司匹林和氯吡格雷联合应用21 d对骨髓中性粒细胞炎症因子及脑内小胶质细胞极化的影响。 方法 雄性C57BL/6小鼠随机分为对照组、阿司匹林联合氯吡格雷组（双抗组）2组，每组10只。通过结扎大脑中动脉远端制作脑缺血再灌注模型（缺血60 min，解开结扎实现再灌注），再灌注即刻通过灌胃给予饮用水100 μL（对照组）和阿司匹林（剂量12 mg/kg，每只实际用量0.3 mg）与氯吡格雷（剂量12 mg/kg，每只实际用量0.3 mg）混悬液100 μL（双抗组），每日1次 ...
董雯, 姜鸣钰, 陈文涛, 刘文倩, 陈青芳, 赵顺英, 叶维贞, 温少红, 刘向荣
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Rare Metals, Volume 45, Issue 5, May 2026.ABSTRACT The efficacy of immunotherapy in triple‐negative breast cancer (TNBC) is often limited, whereas the immunotherapeutic potential of pyroptosis is frequently undermined by a compensatory surge in mitophagy. To address this challenge, this study developed a reactive oxygen species (ROS)‐responsive and mitochondria‐targeted nanomicelle (POM@CTT ...
Qingqing Dou +6 more
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Rare Metals, Volume 45, Issue 4, April 2026.ABSTRACT Diabetic wound infections are a common complication of diabetes, which severely impact patients' quality of life. Effective treatment of diabetic wound infections remains one major challenge in the clinic, partially due to the formation of bacterial biofilm and antibiotic resistance.
Liang Zhou +8 more
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Rare Metals, Volume 45, Issue 4, April 2026.ABSTRACT Constructing a porous scaffold with the essential features of natural cancellous bone, including structural architecture and biochemical cues, is one of the pivotal factors for effective bone regeneration. In this study, a biomimetic composite porous Gelatin fibrous scaffold (SrHA/Gelatin) incorporating ultralong strontium‐doped hydroxyapatite
Yin‐Chuan Wang +8 more
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四川大学学报. 自然科学版, 2012 用抗β8整合素单克隆抗体,检测星形胶质细胞氧糖剥夺-复氧模型,小鼠死后不同时间肾和脑组织β8整合素表达、定位的变化,绘制光密度平均值-死亡时间曲线,初步推断小鼠个体死亡时间.结果显示缺氧环境中,星形胶质细胞、肾小管上皮细胞和脑胶质细胞β8整合素表达量升高.细胞形态的变化以脑胶质细胞最为明显,β8整合素定位由细胞膜到细胞浆,最后随细胞膜破裂释放到细胞外基质.Image-Pro Plus分析显示,缺氧脑胶质细胞β8整合素表达量在48 h达最高值,绘制的光密度平均值-死亡时间曲线能初步推断小鼠个体死亡的时间.
黎光 +6 more
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Zhongguo shiyan zhenduanxue, 2009 目的观察细胞因子IL-1α与TGF-β对兔角膜基质细胞胶原降解的作用,并探讨其相互作用对角膜基质细胞胶原分解代谢的影响。方法角膜基质细胞表面添加含纤溶酶原及不同浓度的IL-1α和/或TGF-β的MEM液,培养24小时,测定培养液中的羟脯氨酸含量作为胶原降解活性。结果IL-1α促进角膜基质细胞胶原降解;TGF-β1抑制角膜基质细胞的胶原降解,IL-1α与TGF-β同时存在,在IL-1α低浓度时,抑制角膜基质细胞胶原降解,高浓度时,TGF-β1不能抑制角膜基质细胞胶原降解。结论IL-1α与TGF ...
姜耀和 +3 more
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