目的 肝血窦内皮细胞(liver sinusoidal endothelial cells, LSEC)是肝内特化的内皮细胞,其独特的窗孔结构是肝脏稳态维持的必要条件。LSEC去窗孔化是促进肝纤维化发展的重要因素,而基质硬度和膜张力等力学因素能否直接调控LSEC去窗孔化目前尚不清楚。方法 构建小鼠肝纤维化模型及硬度可调的水凝胶,并采用原子力显微镜量化窗孔的数量和大小,分析基质硬度调控LSEC去窗孔化的力学转导机制;通过改变溶液渗透压调节细胞膜张力,使用Flipper-TR探针量化膜张力的变化 ...
龙勉, 张晓宇, 李宁
core
<正>蓝藻的暴发,已形成了一种自然灾害。水环境问题正面临着一场严峻的挑战。蓝藻的控制方法有多种,归结起来可分为:生物操纵法、絮凝沉淀或絮凝气浮物化法、机械清除法、化学杀灭法等。在蓝藻大量发生或因风力原因,在湖泊造成蓝藻水华堆集时,靠生物或微生物等方法难以控制,可采用机械收获的方法直接将蓝藻收出,机械除藻能在短期内减少蓝藻生物量、降低内源水体氮 ...
沈银武, 刘永定, 李敦海
core
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