目的探讨广州管圆线虫来源的Acan-Gal-1调控巨噬细胞免疫表型和Annexin A2/S100A10膜表面易位的分子机制。方法采用IFA方法检测巨噬细胞中Annexin A2和S100A10的定位;采用流式细胞术和ELISA检测巨噬细胞功能变化;通过siRNA干扰Annexin A2,检测Acan-Gal-1对各指标的影响。通过Western blot、qRT-PCR等方法检测相关基因和蛋白的表达。通过生物信息学方法分析Acan-Gal-1蛋白结构特征,利用软件进行序列比对,预测其与Annexin ...
谢丽莎 +4 more
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