[Variant Allele Frequency Stratification and EGFR Co-mutation Characteristics in AKT1 E17K-mutant Non-small Cell Lung Cancer]. [PDF]
Zhao D +5 more
europepmc +1 more source
二种淡水微囊藻rDNA16S-235基因间隔区的序列测定与分析
本研究采用PCR及序列测定的方法,对我国淡水铜绿微囊藻有毒株(M8641)和另一低毒的种类惠氏微囊藻(M574)rDNA16S-23S基因间隔区进行了序列的测定和分析,结果表明:rDNA16S-23S基因间隔区可以作为一个精细且稳定的指标,用于微囊藻的分类和鉴定。并从分子水平提出了铜绿微囊藻与惠氏微囊藻在种系发生上有较近缘的关系。本文首次对微囊藻属Microcystis rDNA基因间隔区全序列作了报道 ...
陈月琴,何家菀,庄丽,曾陇梅,屈良鹄
core
[State Changes and Stability Grading of Driver Genes in Non-small Cell Lung Cancer Based on Repeated NGS Testing]. [PDF]
Zhao D +10 more
europepmc +1 more source
[Clinical and genetic characteristics and follow-up of 12 children with hypophosphatasia]. [PDF]
Wu S +8 more
europepmc +1 more source
[Classification of systemic lupus erythematosus resting-state functional magnetic resonance imaging data based on the end-to-end model]. [PDF]
Ma Y +8 more
europepmc +1 more source
基于S6K1基因部分序列的鲤科鱼类系统发育关系及内含子1Indel位点分析
鲤科鱼类在东亚分布广泛且数量丰富,在物种演化上具有重要的系统发育地位.本研究基于S6K1基因5′端功能调控序列,通过PCR扩增、克隆和测序,共获得30种鱼类S6K1前端部分DNA序列(外显子1,外显子2及内含子1),对序列变异进行分析,并采用邻接法(NJ)、最大简约法(MP)、最大似然法(ML)和贝叶斯法(Bayesian)重建鲤科鱼类系统发育关系.亚口鱼科的胭脂鱼(Myxocyprinus asiaticus)作为外类群,通过4种方法所得系统发育分支图大致相同 ...
王绪祯 +4 more
core
[Expression characteristics and clinical significance of <i>ERBB2</i> in bladder urothelial carcinoma based on the TCGA database]. [PDF]
Zheng G, Peng Y, DU Y, Xu T.
europepmc +1 more source
3-磷酸甘油醛脱氢酶(GAPDH)是糖酵解途径中的一个关键酶,由于其基因表达量相对恒定,因此在定量和半定量PCR试验中常被用作内源参照基因来确定目标基因的相对表达量。从巴夫杜氏藻(Dunaliella parva)中克隆GAPDH基因,并对基因序列进行了分析。以巴夫杜氏藻cDNA为模板,根据GAPDH的保守序列设计引物,PCR扩增获得了673 bp的cDNA序列。在此基础上,采用cDNA末端快速扩增(RACE)技术,获得了913 bp的3′-cDNA序列,进而获得了cDNA部分序列1 393 bp ...
朱顺妮 +3 more
core
[Insights into the occurrence and heterogeneity of gastric cancer from the perspective of clinical data integration]. [PDF]
Huang Y, Yang S, Ding X, Chu F, Chen R.
europepmc +1 more source
[Preparation of HLYVSPW peptide-displaying outer membrane vesicles and validation of their in vitro targeting effects on triple-negative breast cancer cells]. [PDF]
Wang Q, Zhao S, Wang H, Li X, Ren W.
europepmc +1 more source

