Results 21 to 30 of about 8,383 (159)

重组DNA技术斑点杂交法检测生物检材的性别 [PDF]

open access: yes, 1992
介绍了用斑点杂交技术,以Y染色体特异的3.5kb DNA片段为探针检测人血痕、人的组织碎块、牙齿、头发、羊水等的性别。样本经制备相当于0.1ul新鲜血的血痕,0.05 mg的组织块,带毛囊的头发1根,1/20颗牙齿牙髓质和2ul的羊水经本法检查,均能确定其性别。组织块经用福尔马林固定1周后,同样可以判断其性别,血痕性别的盲测结果全部正确。同时,对9种常见动物的血痕亦作了检查,均未显示阳性反应。本法准确灵敏,操作简单 ...

core  

Multi‐barcoding‐based species identification using a hierarchical attention network with staged curriculum learning

open access: yesMethods in Ecology and Evolution, Volume 17, Issue 4, Page 1143-1160, April 2026.
Abstract DNA barcode‐based species identification has become pivotal in biodiversity research. Current barcoding‐based deep learning methods have advantages in species identification but exhibit significant limitations. By using COI barcoding alone, previous studies were unable to fully capture species identification features. Prior methods showed that
Bin Ye   +4 more
wiley   +1 more source

The Intersexual Differences of Sexual Dimorphism,Feeding Habits and Locomotor Performance at Different Temperatures of Southern Grass Lizard(Takydromus sexlineatus) in Zhaoqing,China [PDF]

open access: yes, 2014
通过测量头、尾、体和胃检,研究肇庆南草蜥TAkydrOMuS SEXlInEATuS个体发育过程中两性异形和食性差异。南草蜥成体个体两性大小无显著差异,雄性头部显著大于雌性,两性头部大小异形可能是受到性选择压力的结果。雌雄个体食物生态位重叠度较高,两性生态位差异微弱,食性差异与两性头部异形无明显相关性。通过设计13种不同的环境温度,研究体温变化对南草蜥两性个体运动能力表现的影响。结果显示环境温度通过影响体温而影响南草蜥的运动表现,两性个体疾跑速均具有在低体温范围内随体温升高而加快 ...
刘文华   +4 more
core   +2 more sources

PCR在检测人类发干性别中的应用 [PDF]

open access: yes, 1993
从1~2cm人发干中提取基因组DNA,以募核苷酸ya、y4_为引物,经过聚合酶链反应,扩增Y染色体特异的片段(446bP)。根据有无该片段的存在来确定发干的性别。这种方法取材方便,灵敏度高、易于推广。本文还报道了科用本方法检测某案例中5根发干的性别 ...

core  

Using miniaturized laboratory equipment and DNA barcoding to improve conservation genetics training and identify illegally traded species

open access: yesConservation Biology, Volume 40, Issue 2, April 2026.
Abstract Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high‐throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades.
Maria Joana Ferreira da Silva   +9 more
wiley   +1 more source

Sex identification methods in ardeid birds and sex ratio in the nestling populations of Little Egret and Chinese Egret [PDF]

open access: yes, 2007
本实验在筛选鹭科鸟类性别鉴定分子标记的基础上,利用这种方法检测白鹭和黄嘴白鹭雏鸟种群的性别比例,分析白鹭和黄嘴白鹭的雏鸟性别与产卵顺序之间的关系,并进一步分析鹭科鸟类W染色体以及Z染色体的CHD片段序列并尝试用于亲缘关系及系统进化的研究。本文以鹳形目5种鹭科鸟类和1种鹮科鸟类的组织为实验材料,采用PCR方法扩增其性别基因相关片段,探讨鹭科鸟类性别的分子鉴定方法。通过测定3对已知性别的白鹭(Egrettagarzetta)、岩鹭(Egrettasacra)和黄嘴白鹭(Egrettaeulophotes ...
江杉
core  

A Multilevel Perspective on Governance and System Integration in Large‐Scale Civil Infrastructure Systems: The Cases of Crossrail and HS2

open access: yesJournal of Critical Infrastructure Policy, Volume 7, Issue 2, Fall/Winter 2026.
ABSTRACT This study explores governance and systems integration challenges in complex infrastructure megaprojects through a comparative qualitative analysis of the UK's Crossrail and High Speed 2 programs. Drawing on project documentation and interview evidence, it examines how strategic pressures, institutional governance arrangements, and project ...
Seraj Kindakji, Mikela Chatzimichailidou
wiley   +1 more source

Acid‐Etched Co‐Vacancy‐Tunable CoFeP/N,P‐Codoped Carbon Nanocages for Highly Efficient Alkaline Water Electrolysis

open access: yesRare Metals, Volume 45, Issue 3, March 2026.
ABSTRACT Efficient and durable bifunctional electrocatalysts are vital for scalable water electrolysis. Here, we realize cobalt‐vacancy (Covac) engineering in CoFeP supported on N,P‐codoped carbon nanocages (Co1‐vacFeP/NPC) through a simple route comprising a CoFe Prussian blue analog precursor, phosphidation–pyrolysis, tannic‐acid etching, and a ...
Yuan‐Liang Yuan   +6 more
wiley   +1 more source

Novel Non‐Equimolar High‐Entropy Zirconates With Superior Thermophysical Properties

open access: yesRare Metals, Volume 45, Issue 2, February 2026.
ABSTRACT Rare‐earth zirconates (RE2Zr2O7) are promising candidates for topcoat materials in thermal barrier coatings (TBCs). However, their practical application in TBCs is limited by poor fracture toughness and low thermal expansion coefficient (TEC).
Taoyi Lu   +7 more
wiley   +1 more source

犀牛角仿冒品研究现状

open access: yes野生动物学报, 2021
本研究总结了犀牛角仿冒品的研究现状。当前犀牛角的仿冒品主要包括以下几类:洞角、蹄匣、纤维聚合物、真假结合仿品和古犀角等。在这些仿冒品中,洞角的组织特征与犀牛角差别较大,鉴别二者相对容易;蹄匣、纤维聚合物、真假结合仿品和古犀角等特征与犀牛角存在很大的相似性,鉴定时存在一定难度。本研究分别指出了仿冒品与真犀牛角之间的差异或应采取的鉴定方法,将对从事犀牛角鉴定的工作人员提供一些参考。
王震
doaj  

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