Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 研究荧光素酶作为报告基因在活体定量和定位监测转基因人胚胎干细胞移植的动向的可行性65377; 【方法】 酶切pGL3 base质粒获得荧光素酶基因,酶切LTBR-uGIP获得载体骨架,连接荧光素酶基因和载体骨架构建慢病毒载体pULP,按照常规方法包装和扩增65377;ULP转导293T细胞以检测其荧光素酶的表达65377;人胚胎干细胞H9 (P40)以W3R细胞为饲养层常规培养,人胚胎干细胞转导iDuet101-IDO (吲哚胺2,3双氧化酶,IDO) 或iDuet101-CTLA4Ig ...
doaj
[A real-world study of clinical cure in children with HBeAg-positive chronic hepatitis B from the Guangxi region]. [PDF]
Zhonghua Gan Zang Bing Za ZhiLyu RY +7 more
europepmc +1 more source
[Acute<b>-</b>on<b>-</b>chronic liver failure induced by hemochromatosis in a patient with post<b>-</b>hepatitis B cirrhosis: A case report and literature review]. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue BanCai Y, Zhang D, Chen Q.
europepmc +1 more source
[Guillain-Barré syndrome following allogeneic hematopoietic stem cell transplantation in a pediatric patient: a case report and literature review]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiLyu JY +5 more
europepmc +1 more source
[Construction of a highly efficient HBV infection cell model based on HBV co-receptor neuropilin-1]. [PDF]
Zhonghua Gan Zang Bing Za ZhiLiu ZH, He XM, Xu KX, Yu HB, Chen J.
europepmc +1 more source
[Analysis of the Advantages and Clinical Validation of Real-Time Fluorescence Nucleic Acid Isothermal Amplification Detection Technology in Detecting Low Viral Load Samples From HBV-Infected Patients]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanHuang Q +5 more
europepmc +1 more source
[The application of nanoparticles in the treatment and prevention of pathological scars]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiHuang Y, Zhao L, Jin Z, Cui A.
europepmc +1 more source
慢病毒介导shRNA静默CXCR4对Eca109细胞转移特性的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 新近研究显示CXCL12/CXCR4通路在食管癌组织中有较高的表达,本研究探讨应用慢病毒介导的RNA干扰技术,有效静默食管癌细胞株Eca109细胞CXCR4的表达,研究CXCL12/CXCR4通路对食管癌Eca109细胞转移能力的影响65377;【方法】 以人CXCR4 mRNA编码序列作为干扰靶点,构建靶向CXCR4的vshRNA表达质粒,另构建不针对任何已知mRNA的阴性对照vshRNA表达质粒,转染相应组别细胞65377;研究分为控制组65380;阴性对照组及静默组65377 ...
doaj
[Clinical analysis of six patients with pyruvate kinase deficiency caused by <i>PKLR</i> variants]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiWang W +6 more
europepmc +1 more source
[Key epidemiological data and recommended prevention and control measures for primary liver cancer]. [PDF]
Zhonghua Gan Zang Bing Za ZhiCao GW.
europepmc +1 more source