Results 21 to 30 of about 256,919 (167)
Zhongguo shiyan zhenduanxue, 2011 目的本实验以药物磷酸氯喹干预白血病细胞株,观察其对白血病细胞生长凋亡的影响,同时研究用药后凋亡相关蛋白Pnas-2的变化,以探寻磷酸氯喹作用白血病细胞株机制。方法 MTT法检测药物干预后白血病细胞增长情况;AnnexinⅤ/sytox标记细胞以流式细胞仪检测药物干预前后细胞凋亡情况的差别;激光共聚焦观察蛋白Pnas-2的亚细胞定位,蛋白印迹实验检测药物作用前后,Pnas-2表达量的变化。结果 50μg/mL的磷酸氯喹能够诱导白血病细胞株凋亡。并发现凋亡相关蛋白Pnas ...
刘佳 +6 more
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Long‐term use of lokivetmab in dogs with atopic dermatitis
Veterinary Dermatology, Volume 35, Issue 6, Page 683-693, December 2024.Background – Lokivetmab, a caninised monoclonal antibody against interleukin (IL)‐31, is an effective treatment for the pruritus associated with canine atopic dermatitis (cAD). Objectives – To investigate the efficacy and safety of lokivetmab during long‐term treatment defined as at least three consecutive lokivetmab injections in atopic dogs under ...
Bettina Kasper +2 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2002 【目的】探讨细胞间黏附分子-1(CD54)在恶性血液病的表达。【方法】用免疫荧光方法检测19 例急性髓系白血 病, 34 例急性淋巴细胞白血病, 10 例慢性粒细胞白血病, 15 例多发性骨髓瘤, 21 例非霍奇金淋巴瘤和16 例正常人骨髓单个核 细胞表达CD54 。【结果】①除多发性骨髓瘤、慢性粒细胞性白血病外, 急性髓性白血病细胞、非霍奇金淋巴瘤、急性淋巴细胞 白血病患者骨髓单个核细胞表面CD54表达低于正常人。②CD54在急性早幼粒细胞白血病(M3)表达高于在其它类型急性髓 ...
童秀珍 +5 more
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Veterinary Dermatology, Volume 35, Issue 6, Page 595-604, December 2024.Background – Mycobacterium cell wall fraction (MCWF) is derived from nonpathogenic Mycobacterium phlei and is used as an immunomodulatory compound in clinical practice, yet its mode‐of‐action requires further research. Objective – To evaluate the host response to MCWF in canine peripheral blood mononuclear cells (PBMCs) by using enzyme‐linked ...
Robert Ward +9 more
wiley +1 more source
野生动物学报, 2014 本研究测定了石林龙晖野生动物科研中心饲养的非洲白犀牛的红细胞数、血红蛋白、红细胞比积、平均红细胞体积、平均血红蛋白含量、平均血红蛋白浓度、红细胞宽度变异系数、红细胞宽度标准差、血小板数、平均血小板体积、白细胞数、淋巴细胞绝对值、中间细胞绝对值、粒细胞绝对值、淋巴细胞百分比、中间细胞百分比、粒细胞百分比、大血小板百分比、血小板比积、血小板分布宽度。通过比较分析,雌、雄犀牛在红细胞数、大血小板百分比差异性显著(0 ...
王 宇
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Zhongguo shiyan zhenduanxue, 2020 目的观察肾细胞癌中Holliday交叉识别蛋白基因和蛋白表达的改变。方法肾癌A498细胞、os-rc-2细胞作为实验组,人肾小管上皮细胞HK2作为对照组,实时荧光定量聚合酶链反应分析Holliday交叉识别蛋白在实验组和对照组的基因表达情况,蛋白印迹分析其蛋白表达情况。结果与对照组人肾近曲小管上皮细胞HK2细胞相比较,os-rc-2和A498肾癌细胞中Holliday交叉识别蛋白基因和蛋白表达水平均明显下降 ...
原江水 +3 more
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Rare Metals, Volume 43, Issue 11, Page 6006-6022, November 2024.Graphical abstract AbstractCisplatin resistance is the main cause for the failure of cancer therapy. To solve the problem, we proposed to develop a novel human serum albumin (HSA) nanoplatform to integrate chemotherapy, photothermal therapy (PTT) and immunotherapy.
Xue‐Yu Man +7 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2006 目的探讨地塞米松对多核白细胞诱导的角膜基质细胞胶原降解作用及机制。方法利用角膜基质细胞三维胶原凝胶培养模型,加入多核白细胞或其培养上清及不同浓度地塞米松,在MEM培养液中培养48 h后,通过测定培养上清液中的羟脯氨酸（HYP）的量作为胶原降解的活性单位。免疫印记法分析MMP活性变化。结果在纤维蛋白溶酶原存在的情况下,多核白细胞单独无胶原降解作用,多核白细胞与角膜基质细胞混合培养,及多核白细胞培养上清均能增加角膜基质细胞胶原降解量,差异有显著性 ...
李勤 +5 more
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Update on the role of cytokines and chemokines in canine atopic dermatitis
Veterinary Dermatology, Volume 35, Issue 1, Page 25-39, February 2024.Background – Cytokines and chemokines play central roles in the pathogenesis of canine atopic dermatitis (cAD). Numerous studies have been published and provide new insights into their roles in cAD. Objectives – To summarise the research updates on the role of cytokines and chemokines in the pathogenesis of cAD since the last review by the ...
Chie Tamamoto‐Mochizuki +6 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2002 【目的】以腺病毒携带的人内皮抑素基因-Endostatin 基因( Ad/hEnd)感染舌癌细胞株 Tca8113 和人脐静脉内皮细胞株 ECV304, 观察其在舌癌细胞和静脉内皮细胞株中的表达。【方法】用 293 细胞扩增Ad/hEnd,并抽提、纯化。以 10 MOI 的 Ad/GFP 感染 ECV 细胞,观 察细胞中表达绿色荧光蛋白的细胞所占的比例。采用免疫组化法检测 Endostatin 蛋白在感染了Ad/hEnd 的 Tca 细胞和 ECV 细胞中的表达Ad/hEnd 感染 Tca ...
李 立 +4 more
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