Results 11 to 20 of about 18,757 (119)
The Making of the Right to Food: Tracing Food Policy Conflict and Negotiated Outcomes in Colombia
ABSTRACT The 2016 Peace Agreement between the Colombian Government and the FARC guerrilla group marked a historic step toward ending more than five decades of armed conflict. It also reshaped national food policy with lasting implications. During the negotiations, deep ideological divisions emerged between food sovereignty and food security, and these ...
Felipe Roa‐Clavijo
wiley +1 more source
目的分析58例中国东北地区非综合征性耳聋(nonsyndromic hearing impairment,NSHI)患儿GJB2基因突变类型和频率。方法收集吉林省吉林市聋哑学校的58例非综合征性耳聋患儿(分别来自57个家庭)及37例听力正常家属的血样,经聚合酶链反应(polymerase chain reaction,PCR)扩增GJB2基因编码区,用酶切方法初步分析已知的233-235位点,进一步行DNA测序证实酶切结果并发现新的突变类型;同时对部分家属进行DNA测序 ...
于飞 +11 more
doaj
ABSTRACT Confrontational violence still puts catastrophic burdens on children, with 2024 being the highest recorded levels of displacement, malnutrition, injury, and deprivation in contemporary humanitarian history. Children's surgical care is still grossly underrepresented in health responses worldwide in the prevention of avoidable deaths and long ...
Gaurav Chanderprakash Mittal +4 more
wiley +1 more source
【目的】构建突变型α-L-艾杜糖醛酸酶基因(IDUA)cDNA, 为体外表达、鉴定突变的性质提供物质基础。【方法】 以野生型pcDNA3-IDUA 为基础质粒, 采用双引物法体外定点诱变技术, 引入突变E404X。【结果】突变区域经PCR-SSCP 和 测序证实诱导突变成功。【结论】本研究成功构建了突变型IDU A cDNA, 可用于下游的体外表达;此定点突变技术具有简便、 诱变效率高等优点, 是体外构建突变型基因的一种有效方法。
于洪枫 +3 more
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ABSTRACT The electrocatalytic co‐reduction of CO2 and nitrate (NO3− ${\text{NO}}_{3}^{-}$) to synthesize urea presents a sustainable alternative to traditional industrial processes, yet its efficiency is fundamentally limited by the kinetically mismatched activation and coupling of CO2 and NO3− ${\text{NO}}_{3}^{-}$.
Jiayu Liu +9 more
wiley +1 more source
【目的】检测中国人粘多糖贮积症Ⅰ 型患者IDUA 基因(IDU A )突变。【方法】采用PCR-SSCP 、PCR 产物直接 测序等技术对35 例粘多糖贮积症Ⅰ 型患者的IDUA 第2 、6 、7 、8、9 和10 外显子及其相邻区域进行突变筛查。【结果】本研究 筛查出7 种突变, 均为单碱基置换。一内含子区突变nt1486C※T ;一中性突变nt1945G※C ;1 种无义突变E404X;4 种错义突 变:F198L、L218V、A361T 和V454I 。【结论】中国人IDUA 在第2 、6 ...
于洪枫, 曾瑞萍, 林群娣
doaj
ABSTRACT Na‐superionic‐conductor‐type (NASICON) Na4MnV(PO4)3 (NMVP) is an appealing cathode for sodium‐ion batteries (SIBs), but its application is hindered by Jahn–Teller distortions, sluggish Na+ kinetics and poor conductivity, leading to rapid capacity fade.
Huan Liu +8 more
wiley +1 more source
摘 要 为了解肝细胞癌(HCC)中p53 基因的突变情况, 收集86 例HCC 手术切除标本, 采用聚合酶链反 应-单链构象多态性(PCR/ SSCP)、聚合酶链反应-限制性片段长度多态性(PCR/ RFLP)和DNA 序列分析, 研 究了p53 基因的突变情况。86 例HCC 中, p53 基因的突变率为36 %, 其中, 密码子249 的突变率为33 .7 %, 1 例 SSCP 和RFLP 均提示密码子249 存在突变者经DNA 序列分析显示密码子249 的第三个碱基发生了AGG/Arg →
彭晓谋 彭文伟 周元平
doaj
Hypertension at 5 months postpartum in women with gestational diabetes
ABSTRACT Objective Gestational diabetes mellitus (GDM) is associated with future maternal cardiovascular disease, independent of the development of Type‐2 diabetes mellitus. As a major cardiovascular risk factor, we examined the incidence and predictors of hypertension at 5 months postpartum in women with previous GDM.
C. Gómez Fernández +7 more
wiley +1 more source
目的分析吉林省耐多药结核分枝杆菌临床分离株rpoB、katG基因突变特点,为建立本省快速耐多药检测方法提供参考。方法对吉林省耐多药结核分枝杆菌菌株扩增rpoB、katG基因测序后比对分析。结果耐多药结核分枝杆菌rpoB、katG基因的突变率分别为83.53%和70.6%,突变类型包括点突变、联合突变和缺失。在rpoB基因中,82.30%突变发生在RRDR区域,最常见突变位点为rpoB 531、rpoB 526、rpoB 516。KatG基因突变率为70.6%,最常见突变位点为katG315 ...
张炜煜 +4 more
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