Results 21 to 30 of about 18,757 (119)

Revitalizing Traditional Tank Systems: Case Study Insights From Sri Lanka's Village Tank Cascade Systems for Climate Resilience and Water Governance

open access: yesWorld Water Policy, Volume 12, Issue 3, August 2026.
ABSTRACT Village tank cascade systems (VTCS) are a long‐established form of water management in Sri Lanka's dry zone. However, their role in groundwater recharge, climate adaptation, and water governance remains insufficiently recognized in policy and planning.
Ananthini Nanthakumaran   +1 more
wiley   +1 more source

K-ras基因突变表达谱与结直肠癌肝转移的相关性分析

open access: yesZhongguo shiyan zhenduanxue, 2011
目的探讨K-ras基因突变谱与结直肠癌肝转移的相关性,为进一步阐明结直肠癌肝转移的分子机制提供理论依据。方法收集2008年1月至2010年8月在我院治疗的结直肠癌肝转移患者30例,进行K-ras基因突变检测,分析K-ras基因的突变类型及频率,探讨K-ras基因的突变谱与结直肠癌肝转移患者性别、年龄、原发灶位置、转移位置、病灶形态及TNM分期的相关性。结果 30例结直肠癌肝转移患者组织中K-ras基因突变者14例,突变率为47%,其中12密码子突变9例,突变率为30%;13密码子突变者5例,突变率为17%
王涛   +4 more
doaj  

Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity

open access: yesNew Phytologist, Volume 251, Issue 3, Page 1283-1293, August 2026.
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng   +4 more
wiley   +1 more source

PCR 技术对Wilson 病基因进行定点突变的研究

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2002
【目的】利用聚合酶链反应(PC R)技术对Wilson 病(WD)基因进行体外定点突变的研究。【方法】采用PCR 定点 突变技术, 首先设计两对引物, 将突变位点设计在引物上, 通过重叠延伸法两次PCR 扩增, 扩增片段上含有所需要的突变位 点, 最后将扩增片段克隆入pRc/ CMV 载体中。【结果】DNA 测序表明在预期位点已经发生突变,WD 基因第778 位密码子由 精氨酸(Arg)残基突变为亮氨酸残基(Leu), 用PCR 定点突变技术成功构建Wilso n 病基因突变体。【结论】PCR ...
闫振文   +4 more
doaj  

Powerful representation of the poor? German welfare associations' narrative advocacy during COVID‐19

open access: yesPolicy Studies Journal, Volume 54, Issue 3, August 2026.
Abstract The COVID‐19 pandemic sparked unprecedented experimentation in the German social assistance system, leading to changes previously considered impracticable by policymakers. This included a sanctions moratorium, easier access to benefits, and temporary cash transfers, all of which were advocated by welfare associations—key organized interests ...
Christopher Smith Ochoa
wiley   +1 more source

21例葡萄糖-6-磷酸脱氢酶缺乏症基因突变型分析

open access: yes四川大学学报. 自然科学版, 2002
G6PD是磷酸戊糖旁路代谢的限速酶 ,也是重要的看家酶 .G6PD缺乏症是人类常见的遗传性疾病 .作者应用突变特异性扩增系统 (ARMS) ,对 2 1例G6PD缺乏症患儿进行基因检测 .结果在2 1例患儿中检出G1388A突变 7例 ,G1376T突变 4例 ,A95G突变 3例 ,未定型 7例 .成都常见的G6PD基因突变型为中国人中常见突变型 ,分析四川人群G6PD基因突变型 ,估计基因突变频率 ,可为四川省G6PD缺乏症的临床防治提供理论依据 .
杨春蕾   +4 more
doaj  

肝硬化患者血浆cfDNA中TERT热点突变率研究

open access: yesZhongguo shiyan zhenduanxue, 2021
目的探讨肝硬化患者血浆游离DNA(cfDNA)中TERT基因-124(C/T)和-146(C/T)热点突变率及其突变与临床指标相关性。方法纳入航天中心医院2019年10月-2020年11月肝硬化患者80例,收集患者临床基本资料。EDTA抗凝全血分离500μL血浆提取cfDNA。TERT热点突变检测采用ARMS-qPCR方法。结果在80例肝硬化患者血浆cfDNA中,检测到TERT热点突变6例(7.5%),其中-124(C/T)4例(5%),-146(C/T)2例(2.5%)。与非突变组比较 ...
薛晓霞, 何美琳, 梁国威
doaj  

Rhetorical Diffusion and Policy Latency: Tracing AIPAC Influence in US–Iran Relations (2010–2026)

open access: yesPolicy Studies Journal, Volume 54, Issue 3, August 2026.
ABSTRACT How do domestic interest groups shape the transition from diplomatic agreements to kinetic warfare? This research article addresses the methodological stagnation in foreign policy analysis by introducing a computational text‐as‐data pipeline to systematically quantify lobbying influence.
Bülent Aras
wiley   +1 more source

Justice in coexistence: Pastoralism and large carnivores on the Qinghai‐Tibet Plateau

open access: yesPeople and Nature, Volume 8, Issue 7, Page 2186-2199, July 2026.
Abstract The conflict between livestock husbandry and large carnivore conservation presents significant challenges in grassland ecosystems worldwide. Achieving sustainable coexistence among herders, livestock and large carnivores requires reconciling diverse perspectives and interests through equitable, inclusive and fair processes that address both ...
Yufang Gao, Yue Yu
wiley   +1 more source

Root economic space is associated with rhizosphere microbial divergence via root metabolite‐mediated pathways in degraded grasslands

open access: yesFunctional Ecology, Volume 40, Issue 7, Page 2068-2082, July 2026.
Read the free Plain Language Summary for this article on the Journal blog. Abstract Grasslands worldwide are experiencing severe degradation due to overgrazing, climate change and anthropogenic disturbances, resulting in substantial declines in biodiversity and ecosystem functioning.
Xiaoqi Wang   +5 more
wiley   +1 more source

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