利用CD23cDNA全基因克隆pUCD976,分别以限制性内切酶EcoRI和XbaI酶切该重组质粒DNA,回收1.0kb的CD23cDNA全基因、HindⅢ酶切后回收3’端607bp的结合区段基因以及EcoRⅠ和HindⅡ酶切回收5’端403bp的调控区段基因。将各回收片段先以Klenow酶补平后,插入已补平的pZIP逆转录病毒载体BamHⅠ单切点,利用地高辛素标记的CD23cDNA全基因探针,以斑点核酸杂交筛选逆转录病毒重组体,结合用限制性内切酶BamHI和/或BglⅡ酶切鉴定插入方向,最终获得正 ...
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Shan B +6 more
europepmc +1 more source
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Yang RY, Feng TZ, Pei SJ, Qi H, Piao HL.
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Liu Y +8 more
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Zhang W +11 more
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Wang KY, Liu L.
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Liao Y, Xiao TT.
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Zheng J, Hu X, Gu B.
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Zhu S, Chen S, Zheng Q, Liu H.
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Li YR, Zhang CL, Wu Y, Liu R, Meng LJ.
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