Results 11 to 20 of about 300 (104)
东北虎(Panthera tigris altaica)是体型最大的猫科(Felidae)动物之一,是极具代表性的珍稀野生动物。现有研究表明,我国野生东北虎种群遗传多样性较低、近交水平较高。尽管目前我国野生东北虎的数量在逐步增长,但通过人工干预来提高野生东北虎的遗传多样性会更利于其数量的恢复,通过野化放归进行遗传拯救是一种关键策略,但实施遗传拯救之前,必须确定圈养个体与现存野生个体间的遗传关系。采用粪便DNA的高通量测序数据组装了51只横道河子圈养东北虎和13只完达山 ...
褚佳宁 +11 more
doaj +2 more sources
Exploratory analysis of gut microbiota differences in childhood asthma with different severity [PDF]
Objective·To explore the characteristics and differences of gut microbiota in children with different severity of bronchial asthma, and build a prediction model of gut microbiota in severe asthma.Methods·In this study, children aged 5 to 14 diagnosed ...
HAN Xiao +3 more
core +1 more source
Invertebrate‐derived DNA (iDNA) has enabled the identification and quantification of ecological interactions within trophic networks and its use in biodiversity assessment. The dung beetle iDNA currently uses live beetles. However, the effectiveness of the iDNA approach hinges on the successful detection of mammalian DNA from specimens collected via ...
Thilina S. Nimalrathna +3 more
wiley +1 more source
Image of Chinese pangolin from study areas (top left), sampling location map (top right). Median joining network of Chinese pangolin population (size of circles represents the relative frequency of that haplotype present in the population). B) Demographic history of Chinese pangolin population estimated using Bayesian skyline plot.
Prajnashree Priyambada +11 more
wiley +1 more source
Highlights For the first time, our study contributed a systematic evaluation of bile acid (BA) profiles in plasma, feces and liver tissue and gut microbiota changes triggered by fasting‐refeeding cycle in mice. Our work clearly demonstrated that overnight fasting leads to significant, but reversible changes in BA profiles and gut microbiota composition.
Yi Zhang +16 more
wiley +1 more source
粪便显微分析和DNA宏条形码技术在野猪食性分析中的应用和比较
有蹄类动物食性分析对基础生态学研究乃至大型濒危食肉动物保护与恢复有重要科学价值。食物组成定量化有很多方法,常用的方法有粪便显微分析和DNA宏条形码技术,但各有其优缺点。2014年12月—2016年3月,在珲春、汪清、黄泥河、天桥岭和穆棱5个地区收集了野猪(Sus scrofa)新鲜粪便样品,运用粪便显微分析和DNA宏条形码技术比较研究了冬季野猪食性。仅对比分析了野猪植物性食物,通过粪便显微分析获得的野猪食物组成包含32属植物,比例大于4%的主要食物有木贼属(Equisetum,(22.76±1.87)%)
宫 爽 +4 more
doaj
小檗碱对口服降糖药糖尿病患者的胃肠激素及肠道菌群的影响 [PDF]
目的:探讨盐酸小檗碱对2型糖尿病(T2DM)胃泌素(GAS)、胰高血糖素(GLC)及肠道菌群的影响。方法:以25例口服降糖药治疗的T2DM患者为治疗组,对照组选取健康人群10例。T2DM患者常规治疗,同时给予盐酸小檗碱500 mg,每日3次,连续12周。分别于0、4、8、12周检测血糖、GAS、GLC,于0、12周采集清晨空腹粪便进行粪便菌群培养。结果:治疗组治疗后FPG及餐后1hPG有不同程度的下降 ...
吴埼淇 +5 more
core +1 more source
探讨分子粪便学在食性分析中的常用技术,概述了各项技术的概念、操作方法和最新进展。结果表明:分子粪便学方法在国外的动物食性研究中应用较多,与传统食性研究方法相比,利用植物DNA进行测序得到的结果更快速、更高效,并可降低动物消化率对结果的影响。建议研究者可以通过结合当地植被情况建立植物DNA条形码数据库对结果进行修正,对于近缘种植物可以采用特异性引物进行区分。
黄乃莹刘艳华
doaj
Health economic evaluation of different colorectal cancer screening strategies in Hubei province: a Markov simulation analysis based on local cancer registration and domestic study data [PDF]
ObjectiveTo construct a Markov decision model for colorectal cancer screening, evaluate the long-term cost-effectiveness of different screening strategies, determine the optimal screening strategy, and provide a theoretical reference for improving ...
Bing MAO +12 more
core +1 more source
Function and mechanism of Repulsive Guidance Molecule RGMb/Dragon during Colorectal Cancer progression [PDF]
结直肠癌是我国甚至全球范围内最常见的肿瘤之一,也是肿瘤相关死亡的主要原因之一。及时而有效的诊断是降低结直肠癌死亡率的重要措施。除了临床上常规的诊断及治疗方法外,人们也在不断致力于新型肿瘤标志物的研究,以进一步明确结直肠癌发生发展的机制。排斥导向分子(RGM)家族成员RGMb,又名Dragon,作为骨形态发生蛋白(Bonemorphogeneticprotein,BMP)通路的辅助受体,不但承载着胚胎发生、神经修复和肾脏正常生理功能,还与各种上皮来源肿瘤的发生发展密切相关。然而,RGMb ...
施颖
core

