【目的】 分析预测申克孢子丝菌cDNA中编号为Locus_168_Contig_1序列编码的基因及蛋白质结构、功能特征,并进行分子克隆。 【方法】 利用NCBI及Expasy网站提供的BLASTx、SignalIP、InterPro Scan等生物信息学软件分析其序列,判断其是否为全长编码序列、同源序列名称,并分析其结构、定位及功能等;将其中的全长编码区利用PCR方法进行扩增后克隆到pET-30a(+)载体,测序验证。 【结果】 该序列是亚精胺合成酶(SPDS)的同源基因,全长序列为1062 bp ...
doaj
[Gandou Bushen decoction ameliorates ovarian injury in murine hepatolenticular degeneration via the AGE/RAGE/NF-κB signaling pathway]. [PDF]
Jiang P, Wu L, Han H.
europepmc +1 more source
[Positive antinuclear antibodies suggest a high inflammatory burden phenotype: a multicenter retrospective analysis of patients with primary biliary cholangitis]. [PDF]
Li SS +18 more
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为了解大熊猫(Ailuropoda melanoleuca)肠道脱落细胞中线粒体DNA(mtDNA)的异质性特点及其与年龄的关系,以92只不同年龄的大熊猫粪便为材料,通过扩增子高通量测序研究非编码的D-loop区、编码rRNA的16S rRNA区以及编码蛋白质的ND1和ND2区的异质性。结果显示:D-loop区存在3个异质性热点区段,突变碱基的数量远远高于其他3个编码区;D-loop区TS/TV小于16S rRNA和ND两个编码区,表明后两者较D-loop区受到更强烈的纯化选择 ...
李晓达 +8 more
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[Effects of mitochondrial transplantation on full-thickness skin defects in diabetic rats]. [PDF]
Li YQ +7 more
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[He<b>'</b>s Yangchao recipe ameliorates premature ovarian insuffi-ciency by regulating 8-oxoguanine DNA glycosylase 1 in mice]. [PDF]
Hu R +7 more
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[<i>Huayu Tongbian</i> Decoction promotes efferocytosis of interstitial Cajal cells in rats with slow transit constipation by inhibiting the PI3K/Akt signaling pathway]. [PDF]
Qiu J +6 more
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[Efficacy analysis of electrochemical detection of leukocyte esterase based on glucosyl ester in diagnosis of periprosthetic joint infection after knee arthroplasty in rabbits]. [PDF]
Luo N +5 more
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[Development of a predictive model for severe <i>Mycoplasma</i><i>pneumoniae</i> pneumonia in children younger than 5 years]. [PDF]
Li YT, Long XY, Zhou J, Zhang HY.
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[The miR-30b/USP14 axis regulates mitophagy via a non-PINK1/Parkin pathway in neuronal oxygen-glucose deprivation/reoxygenation injury]. [PDF]
Bei YH, Yu K, Wang X.
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