Zhongguo shiyan zhenduanxue, 2011 目的运用实时定量PCR（quantitative real-time,qRT-PCR）检测结核分枝杆菌蛋白Ag85B、38kDa及MPT64编码基因的表达水平,探讨结核分枝杆菌耐药菌株和敏感株,北京基因型和非北京基因型的蛋白抗原在转录水平的差异性及qRT-PCR诊断活菌的价值。方法根据Genbank提供的序列,设计目的基因fbpB、psts1、mpt64及内参基因Sigа的特异性引物,将扩增片段克隆入载体pMD18-T simple,重组质粒经纯化及倍比稀释,应用SYBR ...
陈伟 +7 more
doaj
Zhongguo shiyan zhenduanxue, 2015 目的研究紫檀芪在体外循环术中作为心脏冷停搏灌注液的添加剂使用,对抗体外循环术中心肌缺血、再灌注损伤的作用。方法采用2-3月龄的幼猪,体重10.4-11.5kg,30只,随机分为三组,每组10只。组1为对照组,常规体外循环;组2常规体外循环,心脏冷停搏液中添加紫檀芪;组3常规体外循环,在心脏停跳前15min,静脉输入N’-硝基-L-精氨酸甲酯盐酸盐（L-NAME）,心脏冷停搏液中加入紫檀芪。测定三组动物血清肌酸磷酸激酶同工酶（CK-MB）水平及心肌组织髓过氧化物酶（myeloperoxidase,MPO ...
吕民, 李哲, 赵冬梅
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【【目的】 探讨survivin基因表达对人肺纤维母细胞（humanlungfibroblast，HLF）增殖、凋亡的影响。【方法】 构建survivin基因真核表达质粒peDNA3．1／SVN；利用脂质体转染法将其转入不表达survivin mRNA的HLF细胞系。连续培养10周。筛选出稳定表达的阳性克隆（HLF／SVN）及转空质粒对照（HLF／K）。用RT-PCR、免疫组化、Western blot检测细胞中survivin、bel-2和caspase3mRNA表达：通过观察细胞生长曲线 ...
顾霞 +5 more
doaj
[Protective effect and mechanism of TSPAN9-mediated mitocytosis in interleukin-1β-induced rat chondrocyte senescence]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiChen Q, Da W, Luo N, Shen B.
europepmc +1 more source
[ROMO1 Promotes the Repair of Radiation-induced Skin Injury by Regulating Mitochondrial Function and Keratinocyte Proliferation]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanYang T +8 more
europepmc +1 more source
[Intraperitoneal administration of Allium macrostemon-derived carbon quantum dots alleviates cisplatin-induced acute kidney injury and restores mitochondrial function in mice]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoYang J +7 more
europepmc +1 more source
[Eurycomanone inhibits renal ischemia/reperfusion-induced mitochondrial dysfunction and inflammation in mice by binding to STAT3 to inhibit its phosphorylation]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLiu Z +8 more
europepmc +1 more source
基于NOD样受体热蛋白结构域相关蛋白3炎症小体信号筛选猴痘病毒调控炎症的关键蛋白
Zhongshan Daxue xuebao. Yixue kexue ban目的筛选并鉴定猴痘病毒（MPXV）调控NOD样受体热蛋白结构域相关蛋白3（NLRP3）炎症小体信号的关键蛋白。方法以MPXV国内流行毒株的基因组为模板，构建可能具有炎症调控功能的病毒基因表达质粒库（共82个）。将NLRP3、凋亡相关斑点样蛋白（ASC）、半胱氨酸蛋白酶-1（Caspase-1）、含有荧光素酶报告基因的白细胞介素1β前体（pro-IL-1β-Luc）蛋白的真核表达质粒以及病毒基因表达质粒共转染HEK293T细胞，通过检测细胞上清液中的荧光素酶活性来监测NLRP3炎症小体信号通路的活化 ...
罗铭兴 +6 more
doaj
[A preliminary study on the role of platelet mitochondria in the proliferation and metabolism of multiple myeloma cells]. [PDF]
Zhonghua Xue Ye Xue Za ZhiZhang LY, Xiang YH, Li YY, Zhang J.
europepmc +1 more source
[<i>Qingshen</i> Granules inhibits renal fibrosis in mice by regulating glycolytic reprogramming and H3K18 lactylation]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoChen Y +8 more
europepmc +1 more source