[Shihu Mixture alleviates diabetic cardiomyopathy in rats by Sirt3-mediated upregulation of myocardial mitochondrial mitophagy pathway]. [PDF]
Lin X, He Y, Shi H, Liu J, Hu H.
europepmc +1 more source
[The role and mechanism of chemokine network in promoting osteoarthritis progression by regulating synovial macrophage heterogeneity]. [PDF]
Lin Y, Wu L, Chen Q, Shen B.
europepmc +1 more source
[Exploring the research on the molecular mechanisms of autoimmune liver diseases]. [PDF]
Liu YQ, Jiang XW, Li Y, Liu XL.
europepmc +1 more source
人血管内皮生长因子165慢病毒载体的构建及其抗放射所致细胞凋亡的作用
【目的】 构建人血管内皮生长因子165(hVEGF165)慢病毒表达载体pCDH-CMV-MCS-EF1-copGFPhVEGF165,并转染至HT22 细胞,并进一步观察其对放射线损伤所致的细胞凋亡的保护作用【方法】 PCR扩增hVEGF165基因,克隆到pCDH-CMV-MCS-EF1-copGFP载体;通过酶切电泳,菌落PCR初步筛选和测序鉴定构建的pCDH-CMV-MCS-EF-GFP1-hVEGF165重组载体;采用磷酸钙共转染法转染293T细胞包装制备慢病毒液,并感染HT22细胞 ...
doaj
[Pathogenic mechanisms of Leber hereditary optic neuropathy caused by m.3472T>C mutation]. [PDF]
Zhang H, Shan W, Yasheng M, Guan M.
europepmc +1 more source
【目的】 分析预测申克孢子丝菌cDNA中编号为Locus_168_Contig_1序列编码的基因及蛋白质结构、功能特征,并进行分子克隆。 【方法】 利用NCBI及Expasy网站提供的BLASTx、SignalIP、InterPro Scan等生物信息学软件分析其序列,判断其是否为全长编码序列、同源序列名称,并分析其结构、定位及功能等;将其中的全长编码区利用PCR方法进行扩增后克隆到pET-30a(+)载体,测序验证。 【结果】 该序列是亚精胺合成酶(SPDS)的同源基因,全长序列为1062 bp ...
doaj
[Nanomaterial-driven Lateral Flow Immunoassay: Technological Development and Application]. [PDF]
Zheng J, Hu X, Gu B.
europepmc +1 more source
[Knockdown of <i>Cav1</i> inhibits mitochondrial function and mRNA m<sup>6</sup>A modification and expression of key genes in mouse hepatocytes]. [PDF]
Ding S +4 more
europepmc +1 more source
[Research advances on the targeted programmed cell death regulatory network for treatment of refractory diabetic wounds]. [PDF]
Zhang ZH, He WF.
europepmc +1 more source
[Progress in regulating stem cell functions for repair and regeneration of craniomaxillofacial tissues]. [PDF]
Zhang H, Yang F, Yang R.
europepmc +1 more source

