Results 31 to 40 of about 691,323 (152)
, 1992 3×10~(-4)mol/l亚硒酸钠处理人胃腺癌MGc80-3细胞后,线粒体结构发生了明显变化:线粒体结构典型,多呈短棒状,大小较一致,分布均匀,线粒体嵴明显增多,密度和长度增大,排列方向较一致,线粒体空泡化和嵴扩张现象显著减少,表现出与人正常胃粘膜原代培养细胞线粒体基本相似的特征,进一步分析与线粒体相关的细胞色素氧化酶活性,发现亚硒酸钠可以显著提高该酶活性,由此表明,亚硒酸钠通过改变胃癌细胞线粒体的恶性结构,使线粒体功能恢复正常,调节了细胞代谢机能 ...
汪德耀, 李祺福, 吴乔
core
Rare Metals, Volume 45, Issue 5, May 2026.ABSTRACT The efficacy of immunotherapy in triple‐negative breast cancer (TNBC) is often limited, whereas the immunotherapeutic potential of pyroptosis is frequently undermined by a compensatory surge in mitophagy. To address this challenge, this study developed a reactive oxygen species (ROS)‐responsive and mitochondria‐targeted nanomicelle (POM@CTT ...
Qingqing Dou +6 more
wiley +1 more source
四川大学学报. 自然科学版, 1996 将Ｓａｕ３ＡＩ部分酶切的凤尾菇线粒体ＤＮＡ插入质粒载体ｐＢＳ＋的ＢａｍＨＩ位点，然后以凤尾菇线粒体ＤＮＡ为探针与重组质粒ＤＮＡ进行斑点杂交，筛选到６个阳性克隆．选取其中２个阳性克隆的插入片段，分别与来自６个属的１３个食用菌品种的线粒体ＤＮＡ进行Ｓｏｕｔｈｅｒｎ杂交，其结果显示两探针能与所有测定的侧耳属品种的线粒体ＤＮＡ杂交，并具有多态性；但与其它属线粒体ＤＮＡ只有不同程度的杂交．两探针中Ｈ４的杂交范围较广，而Ｈ２仅能与部分食用菌的线粒体ＤＮＡ杂交 ...
曾凡亚, 汤海妹, 张义正
doaj
Rare Metals, Volume 45, Issue 5, May 2026.ABSTRACT Additive manufacturing, commonly referred to as three‐dimensional (3D) printing, provides the geometric freedom and multi‐material integration needed to build soft medical robots for minimally invasive interventions, prosthetics, and rehabilitation.
Yao Wang +5 more
wiley +1 more source
Species-specific element of Oryza sativa mitochondria tRNATrp(水稻线粒体tRNATrp种属特异性元件研究)
Zhejiang Daxue xuebao. Lixue ban, 2009 为了研究水稻线粒体tRNATrp的种属特异性元件,在野生型水稻线粒体tRNATrp的基础上,设计并完成了 3种向人tRNATrp的突变,体外转录并用枯草杆菌和人这两种不同种属来源的色氨酰-tRNA合成酶(TrpRS)测定了这些tRNATrp分子的氨酰化活力（Kcat/KM）.结果表明,与野生型水稻线粒体tRNATrp相比,3个突变体被人TrpRS氨酰化的活力分别提高了 354、407和803倍,其中以PMPH3（水稻线粒体tRNATrp的氨基酸接受茎的C2-G71和G3 ...
JINXiao-ling(金晓玲) +1 more
doaj +1 more source
Zhongguo shiyan zhenduanxue, 2012 目的为了研究线粒体DNA在人喉癌细胞的增殖、分化及凋亡中的影响及作用,培养线粒体DNA缺失的喉癌细胞系,为构建融合喉癌细胞,研究线粒体DNA突变和线粒体功能改变与喉癌发生之间的关系奠定基础。方法人喉癌细胞系JHUo11加入10%FBS的RPMI1640培养基中,然后在培养基中加入丙酮酸、尿苷、溴化乙锭（EtBr）以去除线粒体DNA,不加溴化乙锭的o11细胞作为对照。经过90天培养,线粒体DNA缺失的喉癌细胞通过健那绿染色后光镜观察和PCR法鉴定。结果 o11细胞经过75ng ...
王磊 +4 more
doaj
Rare Metals, Volume 45, Issue 4, April 2026.ABSTRACT Zirconium dioxide (ZrO2) thin films are critical materials owing to their thermal stability, mechanical durability, and optical transparency enabling applications in photonic, sensing, and electronic devices. However, achieving uniform deposition via plasma‐enhanced chemical vapor deposition (PE‐CVD) has remained challenging due to poor ...
Nour Manafikhi +4 more
wiley +1 more source
牛磺酸对运动大鼠心肌线粒体游离钙、PLA2及脂质过氧化的影响
Zhongguo shiyan zhenduanxue, 2006 目的探讨牛磺酸对运动大鼠心肌线粒体损伤的保护作用。方法以大鼠力竭运动为模型,观察了牛磺酸对运动后大鼠心肌线粒体脂质过氧化水平、抗氧化酶、游离钙及磷脂酶A2（PLA2）的影响。结果力竭运动可引起大鼠心肌线粒体脂质过氧化水平和磷脂酶A,活性显著升高,心肌线粒体超氧化物歧化酶（SOD）活性和游离钙浓度的显著下降,而牛磺酸可抑制人鼠力竭运动后心肌线粒体脂质过氧化水平和磷脂酶A:活性的显著升高,抑制大鼠心肌线粒体SOD的活性和游离钙浓度的显著下降。结论牛磺酸可减少力竭运动后自由基对心肌线粒体的攻击 ...
张陵, 张钧
doaj
Conservation Biology, Volume 40, Issue 2, April 2026.Abstract Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high‐throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades.
Maria Joana Ferreira da Silva +9 more
wiley +1 more source
Rare Metals, Volume 45, Issue 3, March 2026.ABSTRACT Lung cancer remains the leading cause of cancer‐related deaths worldwide, yet immune checkpoint blockade (ICB) is often compromised by an immunosuppressive tumor microenvironment (TME) with acidosis, hypoxia, and poor T cell infiltration. Here, we develop a TME‐responsive nanoplatform (MgH2@Lip/PD‐L1) that couples hydrogen gas therapy with PD ...
Xuyu Gu +5 more
wiley +1 more source