Zhongguo shiyan zhenduanxue, 2005 目的 探讨小儿病毒感染与微卫星DNA的关系。方法 取经咽拭子病毒分离证实为呼吸道合胞病毒或腺病毒感染的患儿血提取DNA ,用聚合酶连反应对基因座D5S818进行扩增 ,聚丙烯酰胺凝胶电泳分离DNA片段 ,成象系统分析D5S818基因片段长度多态性 ;并分析D5S818基因片段长度多态性与病毒感染临床表现的相关性。结果 发现病毒感染后D5S818基因座等位基因频率与正常对照组有明显差异 ,平均AGAT重复数 ,观察组较对照组略高 ,A GAT重复数
孙利炜 +6 more
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高通量透析对尿毒症维持性血液透析患者甲状旁腺素及心功能的影响
Zhongguo shiyan zhenduanxue, 2011 维持性血液透析患者的死亡原因最多见于心血管并发症,继发性甲状旁腺功能亢进是慢性肾衰竭的常见并发症,此时体内血清甲状旁腺素（iPTH）水平不断升高,iPTH已经被认为是尿毒症中分子毒素,引起尿毒症心肌病的重要危险因素之一。尿毒症患者iPTH大于正常值3倍时,其加重心肌[1]
崔明姬 +3 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 1997 摘 要 采用原位杂交和免疫组化技术,检测20例腮腺淋巴上皮瘤样癌( LELC)组织中EB病毒编码的小 RN A( EBE Rs )、潜伏感染膜蛋白( LM Pl )、溶解感染期立即早期基因编码蛋白ZEB RA、早期基因编码蛋白E AD 、晚期基因编码蛋白VC A和M A。结果EB ERs阳性率为100% ( 20 /20) , LM Pl阳性率为85% ( 17 /20) , ZEBRA 均为阴性, EA-D阳性率85% ( 17 /20) , V CA阳性率60% ( 12 /20) , M ...
刘克拉 宗永生
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[Expression and significance of hypoxia-inducible factor 1α and Bcl-2/adenovirus E1B19kDa-interacting protein 3 in children with traumatic brain injury]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiZhu L +5 more
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[Construction of an adenovirus vector expressing engineered splicing factor for regulating alternative splicing of YAP1 in neonatal rat cardiomyocytes]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2022 Li Y, Zhao Q, Song X, Song J.
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[Research progress on the pathogenesis and clinical treatment of cirrhotic cardiomyopathy]. [PDF]
Zhonghua Gan Zang Bing Za ZhiWang KY, Liu L.
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[Expert recommendations on the management of adverse reactions in pegylated interferon alpha therapy for chronic hepatitis B]. [PDF]
Zhonghua Gan Zang Bing Za ZhiExpert Group on the Management of Adverse Reactions in Pegylated Interferon Alpha Therapy for Chronic Hepatitis B.
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[A real-world study of clinical cure in children with HBeAg-positive chronic hepatitis B from the Guangxi region]. [PDF]
Zhonghua Gan Zang Bing Za ZhiLyu RY +7 more
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[Clinical application of blood purification in treatment of severe adenovirus pneumonia]. [PDF]
Zhongguo Dang Dai Er Ke Za Zhi, 2020 Yang MY +9 more
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重组腺病毒载体Ad-LMP-1的构建及其转染 MSC后成骨活性
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 快速构建大鼠LIM矿化蛋白-1基因重组腺病毒载体,转染MSC,探讨LMP-1基因对MSC成骨分化和成骨活性的影响65377; 【方法】 从大鼠成骨细胞内提取总RNA,并设计LMP-1特异性引物进行RT-PCR,获取LMP-1基因后利用Invitrogen公司的TOPO定向克隆技术创建入门克隆pENTR/D-LMP-165377;转化TOP10细菌后,挑取阳性克隆摇菌提取质粒,入门克隆再与表达载体pAD/CMV/V5-DEST进行同源重组反应得到病毒载体pAD/CMV/V5-LMP-1 ...
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