Results 51 to 60 of about 3,059 (103)

外周血TSGF及hMAM表达指标监测乳腺癌微转移价值初探

Zhongguo shiyan zhenduanxue, 2005
目的探索TSGF和hMAM表达指标对乳腺癌细胞外周血微转移诊断的应用价值。方法分离受检者外周血单个核细胞,TRIZOL液提取总RNA,作RT-PCR并用-βactin监控;确认的cDNA先作普通PCR扩增,再作荧光定量PCR扩增。血清作TSGF检测。结果64例标本有63例确认转录为cDNA,其中乳腺癌43例、良性乳腺肿瘤和正常对照各10例。经普通PCR后再作荧光定量PCR,乳腺癌hMAM表达阳性率为53.3%、良性乳腺肿瘤和正常对照均无阳性,乳腺癌组与良性乳腺肿瘤或正常对照组间hMAM表达有显著差异 ...
董学君, 郑专, 茹金城, 黄黎明, 赵雅清, 陈建军, 王贵珍   +6 more
doaj  

实时荧光定量PCR方法检测乳腺癌患者外周血中uPA基因表达水平及其临床意义

Zhongguo shiyan zhenduanxue, 2009
目的探讨人外周血中尿激酶型纤溶酶原激活物（urokinase-type plasminogen activator,uPA）基因表达水平与乳腺癌临床分期和转移的关系,评价其对乳腺癌辅助诊断及判断预后的临床意义。方法采用实时荧光定量PCR方法检测71例正常人和90例乳腺癌患者外周血中uPA mRNA表达水平。结果97.8%的乳腺癌病人外周血中有uPA基因表达,在正常人外周血中的表达率为40.8%;基因表达水平与临床分期和肿物转移情况相关 ...
刘俊泽, 张青云, 王雅明, 徐建军   +3 more
doaj  

脓毒症大鼠肠道及腹水中菌群变化研究

Zhongguo shiyan zhenduanxue, 2011
目的定量检测脓毒症大鼠肠道主要菌群的变化情况,同时找出引起脓毒症大鼠患病甚至死亡的主要致病菌。方法采用盲肠结扎穿孔法（CLP）制作大鼠脓毒症模型,取腹水进行细菌培养和菌种鉴定;同时取结肠内容物提取DNA,使用荧光定量PCR法对肠道内5种细菌进行定量。结果与对照组相比模型组大鼠肠道内双歧杆菌、乳杆菌数量明显减少,而大肠埃希菌的数量则有所增加。同时大肠埃希菌在腹水中大量繁殖。结论肠道中益生菌数量减少和条件致病菌的大量繁殖是导致脓毒症大鼠发病和死亡的微生态学因素。
刘大全, 李东华, 刘洪斌, 宗春辉, 高巧营   +4 more
doaj  

#br# 慢性乙型肝炎病情再活动时血清HBV DNA 水平的变化

Zhongshan Daxue xuebao. Yixue kexue ban, 2000
【目的】研究慢性乙型肝炎患者 HBV 复制水平与病情活动关系。【方法】采用荧光标记定量 PCR 的方法,测定一 组急性发作的慢性乙型肝炎患者(其中HBeAg 阳性组38 例, 抗HBe 阳性组22 例)病情活动期和恢复期的血清HBV DNA 含量。 【结果】血清HBV DNA 含量在活动期明显高于恢复期(P
周小辉
doaj  

[Diagnostic Value of Serological Markers and Quantitative Real-Time PCR in Patients With Invasive Candidiasis]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Md Ali H, Peng L, Yuan Y, Zhou X, Ma Y.
europepmc   +1 more source

[Anti-inflammatory effects of cell membrane vesicle-mediated delivery of small interfering RNA targeting tumor necrosis factor-α on dental pulp stem cells]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Gao R, Ma T, Wang R, Yin Y, Li R, Wang D, Xia B.   +6 more
europepmc   +1 more source

[miR-488-5p promotes osteogenic and neurogenic differentiation of rat bone marrow mesenchymal stem cells and enhances neuralized bone regeneration]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Zeng L, Cheng K, Liu Z, Li J, Yang J, Jiang T.   +5 more
europepmc   +1 more source

[Effect of interleukin-33 on glucocorticoid-induced osteonecrosis of the femoral head in mice]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Li H, Shen M, Liu H, Zhou Y, Gao F, Cheng L, Wang B, Ma J.   +7 more
europepmc   +1 more source

[The role and molecular mechanism of transcription factor EB and its target genes in multiple myeloma treatment with bortezomib]. [PDF]

Zhonghua Xue Ye Xue Za Zhi
Zhang RJ, Wang ZL, Shi XM, Zhang SY, Wang W, Ma MS, Li C, Gu CH, Zhang ZH.   +8 more
europepmc   +1 more source

[Polyphyllin VII inhibits osteosarcoma xenograft growth in mice by inducing ferroptosis via upregulating SOHLH1]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Xiao D, Tang H, Yang M, Teng H, Liang J, Xie T, Feng W, Liu S, Dai W, Li H, Liu Y.   +10 more
europepmc   +1 more source