Results 1 to 10 of about 1,457,005 (164)

通过瞬间表达测定植物花药花粉特异基因启动子的时空表达性

open access: yes四川大学学报. 自然科学版, 1997
通过瞬间表达快速测定了TA29(烟草花药特异基因)和Zm13(玉米花粉特异基因)启动子在一些植物中的时空表达性,结果表明,TA29启动子在烟草和番茄的花药中具有时空表达性,而Zm13启动子在油菜花粉中无任何表达,这为一些植物构建或转化雄性不育和恢复基因提供了理论依据.此外,还讨论了用基因枪转化的瞬间表达方法 ...
钟蓉   +4 more
doaj   +1 more source

人GM-CSF基因真核表达载体构建与表达 [PDF]

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2007
【目的】构建以基因佐剂hGM-CSF基因为基础的真核表达质粒pIGM-CSF,并在宫颈癌细胞HeLa中表达与鉴定。【方法】采用聚合酶链反应从载体pORF-hGM-CSF中,扩增出hGM-CSF基因,克隆到双顺反子真核表达载体pIRES的多克隆位点B(MCSB)中,构建pIGM-CSF真核表达载体。然后采用脂质体转染法将其转染HeLa细胞,用RT-PCR在RNA水平和ELISA在蛋白水平检测其在真核细胞中的表达。【结果】所克隆hGM-CSF基因片段经测序完全正确;重组质粒转染Hela细胞后,检测有hGM ...
卢燕茹, 江振友
doaj   +1 more source

食管癌组织中ADAMTS1、ADAMTS18基因mRNA的表达及其意义

open access: yesZhongguo shiyan zhenduanxue, 2021
目的探讨ADAMTS1和ADAMTS18基因在食管鳞状细胞癌组织中的表达及其与食管鳞状细胞癌临床病理特征的关系。方法应用半定量逆转录聚合酶链反应(RT-PCR)方法分别检测70例食管鳞状细胞癌组织及癌旁正常组织中ADAMTS1、ADAMTS18的mRNA表达,并分析基因表达与临床病理资料之间的关系。结果食管鳞状细胞癌组织中ADAMTS1、ADAMTS18基因的mRNA表达水平显著低于相应的癌旁正常组织(P0.05),ADAMTS1基因mRNA在有淋巴结转移组的表达量高于无淋巴结转移组(P0.05 ...
尹蕾   +5 more
doaj  

Floral bilateral symmetry: one axis, many details

open access: yesNew Phytologist, EarlyView.
Dorsal petal morphological diversity among monkeyflower species. Summary Symmetry is a major component of floral diversity. Most flowers are radially or bilaterally symmetric, with bilateral symmetry (monosymmetry) having evolved repeatedly from radial ancestors. Since the identification of the dorsal identity gene CYCLOIDEA (CYC) in snapdragon, floral
Ya Min
wiley   +1 more source

Partisanship, Deservingness, and the Attitudinal Policy Feedback Process for Social Policy

open access: yesPolicy Studies Journal, EarlyView.
ABSTRACT In an era of identity‐based partisan polarization, we examine whether social policies can still generate positive attitudinal feedback among beneficiaries. Drawing on nationally representative survey data, we demonstrate that partisanship conditions the policy feedback process through divergent perceptions of group deservingness.
Chris Faricy, Christopher Ellis
wiley   +1 more source

Lignin‐Derived Carbon Quantum Dots as Hole‐Trapping Promoters in LCDs/Bi‐MOF/ZIS With S‐Scheme Heterojunction for Efficient Photocatalytic Hydrogen Peroxide Production in Pure Water

open access: yesRare Metals, Volume 45, Issue 10, October 2026.
ABSTRACT Photocatalytic hydrogen peroxide (H2O2) production from water and oxygen driven by solar energy represents a highly promising green and sustainable strategy. Herein, lignin‐derived carbon quantum dots (LCDs) were strategically introduced at the heterojunction between a Bi‐based metal organic framework (Bi‐MOF) and ZnIn2S4 (ZIS).
Wanpeng Sun   +8 more
wiley   +1 more source

Lanthanide Nanoparticles With Dual Enzyme Mimetic Activities for Targeted Treatment of Gram‐Negative Bacterial Infections

open access: yesRare Metals, Volume 45, Issue 10, October 2026.
ABSTRACT Gram‐negative (G−) bacterial infections, fueled by intrinsic antibiotic resistance stemming from their unique outer membrane architecture, pose a critical global clinical challenge. This study introduces a pioneering dual‐enzyme‐mimetic nanotherapy based on NaYbF4 nanoparticles (NaYbF4 NPs) that achieves synergistic antibacterial and anti ...
Yuxiang Zhang   +6 more
wiley   +1 more source

Modulating Oxygen Vacancies via Ag Induced Interfacial Electron Transfer in SnO2 for CO2 Electroreduction

open access: yesRare Metals, Volume 45, Issue 10, October 2026.
ABSTRACT Regulating oxygen vacancies (Ov) in SnO2 to facilitate high‐efficiency carbon dioxide electroreduction reduction reaction (CO2RR) is still a significant challenge. Therefore, a strategy based on Ag‐induced interfacial electron transfer was proposed to modulate Ov in SnO2 for CO2 electroreduction. The prepared Ag/SnO2 with an optimal Ov content
Haoyu Qi   +7 more
wiley   +1 more source

烧伤创面内表皮生长因子、表皮生长因子受体、C-myc三种基因表达变化的临床观察

open access: yesZhongguo shiyan zhenduanxue, 2010
目的为探讨烧伤创面愈合机理,了解烧伤创面内表皮生长因子基因、表皮生长因子受体基因和C-myc基因的变化,为临床治疗提供依据。方法我们对25例病人烧伤创面的不同部位应用原位杂交方法,观察表皮生长因子基因、表皮生长因子受体基因和C-myc基因在烧伤创面肉芽中心部(肉芽中心部)、肉芽与愈合皮肤的交界部(创面交界部)、愈合皮肤部位(创面愈合部)和自身正常皮肤对照部位内表达强度和分布的特点。结果同一部位烧伤创面内,表皮生长因子基因和C-myc基因在交界部表达最强;烧伤创面中心部位表达次之,烧伤创面愈合部位表达较弱;
谷廷敏   +4 more
doaj  

Competitive Anion Coordination Modulates Anode Interfacial Chemistry for Wide‐Temperature Sodium Batteries

open access: yesRare Metals, Volume 45, Issue 10, October 2026.
ABSTRACT Sodium‐ion batteries (SIBs) offer a promising, cost‐effective alternative for large‐scale energy storage due to the abundance of sodium. However, their practical deployment is challenged by sluggish interfacial kinetics and unstable interphase formation, which severely limit performance across wide temperature ranges. Here, we demonstrate that
Mingxing Zhang   +10 more
wiley   +1 more source

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