Results 11 to 20 of about 1,150,306 (165)
四川大学学报. 自然科学版, 1997 通过瞬间表达快速测定了ＴＡ２９（烟草花药特异基因）和Ｚｍ１３（玉米花粉特异基因）启动子在一些植物中的时空表达性，结果表明，ＴＡ２９启动子在烟草和番茄的花药中具有时空表达性，而Ｚｍ１３启动子在油菜花粉中无任何表达，这为一些植物构建或转化雄性不育和恢复基因提供了理论依据．此外，还讨论了用基因枪转化的瞬间表达方法 ...
钟蓉 +4 more
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食管癌组织中ADAMTS1、ADAMTS18基因mRNA的表达及其意义
Zhongguo shiyan zhenduanxue, 2021 目的探讨ADAMTS1和ADAMTS18基因在食管鳞状细胞癌组织中的表达及其与食管鳞状细胞癌临床病理特征的关系。方法应用半定量逆转录聚合酶链反应(RT-PCR)方法分别检测70例食管鳞状细胞癌组织及癌旁正常组织中ADAMTS1、ADAMTS18的mRNA表达,并分析基因表达与临床病理资料之间的关系。结果食管鳞状细胞癌组织中ADAMTS1、ADAMTS18基因的mRNA表达水平显著低于相应的癌旁正常组织(P0.05),ADAMTS1基因mRNA在有淋巴结转移组的表达量高于无淋巴结转移组(P0.05 ...
尹蕾 +5 more
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Conservation Biology, EarlyView.Abstract Ecological niche models (ENMs) are used to assess the abiotic preferences of species by linking their occurrences to the environmental conditions in which they live. We developed a fossil‐informed ENM framework that integrates mid‐Holocene and modern occurrences to test niche stability and reconstruct abiotic niche characteristics for four ...
Claire. M. Williams +3 more
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Exploring legal‐ and health‐risk messaging to reduce demand for elephant skin
Conservation Biology, EarlyView.Abstract Asian elephants (Elephas maximus) are poached for an illegal trade in their skins, which are used in traditional medicine in Africa and Asia. We explored whether messages about the legal and health risks of using elephant skin for medicinal purposes (stomach illness) could reduce such consumption.
Beilu Duan +10 more
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Partisanship, Deservingness, and the Attitudinal Policy Feedback Process for Social Policy
Policy Studies Journal, EarlyView.ABSTRACT In an era of identity‐based partisan polarization, we examine whether social policies can still generate positive attitudinal feedback among beneficiaries. Drawing on nationally representative survey data, we demonstrate that partisanship conditions the policy feedback process through divergent perceptions of group deservingness.
Chris Faricy, Christopher Ellis
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Zhongshan Daxue xuebao. Yixue kexue ban, 2004 【目的】构建人resistin基因原核表达载体并观察其表达,为开展对resistin的研究打下实验基础。【方法】酚氯仿一步法从一位2型糖尿病合并胆管癌患者腹壁皮下脂肪组织抽取总RNA,RT-PCR法扩增出re-sistin基因cDNA,经测序后,PCR产物亚克隆入T载体,用EcoRI和KpnI分别酶切重组T质粒和原核表达载体pGENKS,然后resistin基因片段和表达载体通过T4DNA连接酶进行定向连接,用核酸内切酶EcoRI、KpnI酶切和测序方法鉴定出重组表达质粒 ...
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New Phytologist, Volume 251, Issue 3, Page 1283-1293, August 2026.Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng +4 more
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Emotion and the Advocacy Coalition Framework: An Affective Dynamics Perspective
Policy Studies Journal, Volume 54, Issue 3, August 2026.ABSTRACT Despite extensive evidence that emotion and cognition are deeply intertwined, the Advocacy Coalition Framework (ACF) lacks an analytically independent emotional mechanism in its causal architecture—an omission that may be particularly consequential in policy subsystems structured around morally charged, identity‐laden policy disputes.
Moshe Maor
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烧伤创面内表皮生长因子、表皮生长因子受体、C-myc三种基因表达变化的临床观察
Zhongguo shiyan zhenduanxue, 2010 目的为探讨烧伤创面愈合机理,了解烧伤创面内表皮生长因子基因、表皮生长因子受体基因和C-myc基因的变化,为临床治疗提供依据。方法我们对25例病人烧伤创面的不同部位应用原位杂交方法,观察表皮生长因子基因、表皮生长因子受体基因和C-myc基因在烧伤创面肉芽中心部（肉芽中心部）、肉芽与愈合皮肤的交界部（创面交界部）、愈合皮肤部位（创面愈合部）和自身正常皮肤对照部位内表达强度和分布的特点。结果同一部位烧伤创面内,表皮生长因子基因和C-myc基因在交界部表达最强;烧伤创面中心部位表达次之,烧伤创面愈合部位表达较弱;
谷廷敏 +4 more
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New Phytologist, Volume 251, Issue 1, Page 207-227, July 2026.Network of VAMP714‐dependent interactions during salt stress. Summary Plant responses to salt stress include an altered root architecture mediated by ion imbalances, reactive oxygen species (ROS) accumulation, and altered hormonal responses. Auxin is implicated in these processes.
Jialei Sun +3 more
wiley +1 more source