Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 应用乙腈(ACN)预处理血清的方法,对肝细胞癌(HCC)患者的血清进行蛋白质组分析,寻找与HCC发病相关的蛋白质65377; 【方法】 收集原发性HCC患者和健康人的血清各12例,首先使用乙腈预处理,然后去除白蛋白和免疫球蛋白等高丰度蛋白质,再进行双向电泳(2-DE)分析,筛选HCC与健康对照血清中有显著性差异的蛋白质斑点,并进行MALDI-TOF/TOF质谱鉴定65377; 【结果】 血清经0%65380;20%和30%浓度的乙腈预处理后,进行2-DE分析发现蛋白质斑点的检出数量分别为532
doaj
[Applications of high performance liquid chromatography-mass spectrometry in proteomics]. [PDF]
Se PuLiu W, Weng LX, Gao MX, Zhang XM.
europepmc +1 more source
[Microbial metaproteomics--From sample processing to data acquisition and analysis]. [PDF]
Se PuWu EH, Qiao L.
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】建立盘状红斑狼疮皮损和正常皮肤角质形成细胞的双向电泳图谱，分析二者蛋白质的表达差异及特点。【方法】运用固相pH梯度双向凝胶电泳分离6例盘状红斑狼疮患者皮损及正常皮肤角质形成细胞总蛋白，考马斯亮蓝染色后用ImageMaster 2D图象软件比较分析，识别差异蛋白。【结果】盘状红斑狼疮皮损和正常对照电泳图谱平均蛋白点分别为1566±32和1682±38，平均匹配点数为1286±14 和1314±21，匹配率为82.12%和78.12%。分辨差异蛋白点共22个，11个在皮损高表达，2个在皮损组低表达 ...
doaj
[Open experiment: quantitative proteomics analysis of thyroid-cancer tissue slices using ultra-high performance liquid chromatography-tandem mass spectrometry]. [PDF]
Se PuLi YL +5 more
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[A highly sensitive approach for the analysis of tyrosine phosphoproteome in primary T cells]. [PDF]
Se PuLiang FC, Ke M, Tian RJ.
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[Deciphering cellular processes responding to lethality of 17β-estradiol by quantitative phosphoproteomics]. [PDF]
Se PuLi Y +5 more
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[Spatial omics in pulmonary fibrosis: advancing mechanistic insights and therapeutic strategies]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanPeng Y, Xie Y, Yang F, Qiao X, Tian Y.
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[Whole genome sequencing and analysis of familial nonsyndromic congenital tooth agenesis]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiZheng Y, Wang D, Jiang T, Yang D, Lu H.
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[<i>Huoxue Qingjie Ling</i> alleviates bile duct ligation-induced hepatic fibrosis in rats by regulating the Sirt1-autophagy signaling pathway]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi C +6 more
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