, 2008 银鲫卵母细胞特异的凝集素(Gibel carp oocyte-specific lectin,GOL)是经差减杂交筛选出来的。本研究采用原核表达方法,利用Pinpoint-T载体表达了该凝集素的部分肽段(从22到214个氨基酸),并经纯化用来免疫家兔制备多克隆抗体。采用免疫荧光共定位方法 ...
王红莹, 桂建芳, 周莉
core
[The application of nanoparticles in the treatment and prevention of pathological scars]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiHuang Y, Zhao L, Jin Z, Cui A.
europepmc +1 more source
[Ophiopogonin D alleviates doxorubicin-induced myocardial hypertrophy in mice by activating the β-catenin/FUNDC1/mitophagy axis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLei Y, Song J, Xu L, Liu R, Zhao Y.
europepmc +1 more source
Snail基因慢病毒载体的构建及其促进结肠癌细胞上皮间质化的研究
Zhongshan Daxue xuebao. Yixue kexue ban, 2012 【目的】 构建Snail基因慢病毒载体,研究Snail基因的表达与结肠癌细胞上皮间质化的关系。【方法】 利用Gateway Technology法构建Snail基因表达质粒和空白对照质粒,行慢病毒包装,成功构建Snail基因慢病毒载体后,在结肠癌细胞系caco2中转染Snail基因质粒及空白载体对照,构建稳转细胞株caco2- Snail和caco2-vc,并在LoVo细胞中瞬时转染上述质粒,对Snail及E-钙黏蛋白(E-cadherin)的表达进行检测,并用划痕 ...
doaj
[Progress in regulating stem cell functions for repair and regeneration of craniomaxillofacial tissues]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZhang H, Yang F, Yang R.
europepmc +1 more source
, 1993 用聚丙烯酰胺梯度凝胶电泳分析了鲢×鳙、鲢×团头鲂的二倍体和异源三倍体杂种,以及同源三倍体鲢及其亲本种的8种蛋白质和同工酶的表型。二倍体杂交子代的基因表达是多样的,这些多样的表达可能与双亲的这些蛋白质和同工酶的表型差异有关。如与二倍体杂交子代相比,鲢×鳙异源三倍体的肌肉蛋白和血清蛋白有较一致的表型。此外在两种异源三倍体中EST和MDH同工酶的表型接近母本。这些结果表明异源三倍体由于增加了一套母本染色体组,基因的表达较接近母本或趋向一致。同源三倍体鲢与二倍体鲢的肌肉蛋白 ...
朱蓝菲 +3 more
core
[Bifunctional biomaterials: An innovative strategy for synergistic bone tumor therapy and bone regeneration]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiFeng W, Chang B, Wang C, Fan B.
europepmc +1 more source
[Dynamic stretching promotes osteogenic differentiation of human bone marrow mesenchymal stem cells in three-dimensional culture]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanBai X, Yuan Z, Zhou Y, Lv L.
europepmc +1 more source
[Long non-coding RNA LASTR promotes progression of head and neck squamous cell carcinoma by binding to miR-4476 and upregulating BCAM expression]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoWu B +6 more
europepmc +1 more source
[Targeted inhibition of miR-503 upregulates Apelin expression to alleviate myocardial infarction in mice]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi J +10 more
europepmc +1 more source