rAAV2/1-SR-BI-IRES-EGFP转染HepG2肝细胞对 125I-HDL摄取的调节
摘 要: 【目的】 探讨B族I型清道夫受体(SR-BI)过表达对高密度脂蛋白(HDL)摄取的调节,为SR-B1在胆固醇逆转运(RCT)及动脉粥样硬化(AS)中的研究提供新思路?【方法】 用含SR-BI基因的重组腺相关病毒rAAV2/1-SR-BI-IRES-EGFP杂合载体转染人HepG2肝细胞株,Realtime RT- PCR和Western Blot分别检测转染后SR-BI mRNA和蛋白的表达?125I-HDL同位素标记观察转染对HDL结合的影响?激光共聚焦显微镜观察转染后SR ...
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