Results 41 to 50 of about 17,301 (184)
重组腺病毒介导RECK基因在大肠癌细胞中的表达及抑制癌细胞浸润能力的实验研究
Zhongguo shiyan zhenduanxue, 2005 目的 研究重组腺病毒介导RECK基因转染大肠癌细胞后基因的表达情况,并观察转染细胞浸润能力变化。方法 构建了RECK基因的复制缺陷型重组腺病毒载体adRECK ,以adRECK转染大肠癌癌细胞株后,用RT PCR法检测RECK基因在大肠癌中的表达。并用Boyden小室测试转染细胞的侵袭性。结果 RECK基因在转染细胞中有效的表达。被转染细胞浸润能力明显下降。结论 重组腺病毒介导RECK基因能够在大肠癌细胞中有效的表达,转染病毒的大肠癌浸润能力明显下降。
杨德君 +4 more
doaj
Curcumin Promoted the Apoptosis of Cisplain-resistant Human Lung Carcinoma Cells A549/DDP through Down-regulating miR-186* [PDF]
, 2010 Background and objective Curcumin, a natural compound, is derived from the rthizom of Curcuma longa. In vitro and in vivo preclinical studies have shown its anti-inflammatory, antioxidant, anticancer activities and so on.
Jian ZHANG, Ni TANG, Yongping DU
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New Phytologist, Volume 247, Issue 5, Page 2086-2097, September 2025.Summary The importance of marine angiosperms, or seagrasses, as foundation species for marine coastal ecosystems is in marked contrast to their low species number of only 70 described taxa. Seagrass species tend to have very similar overall morphologies dictated by hydrodynamic forces of the ocean environment.
Xiaomei Zhang +14 more
wiley +1 more source
Functional Analysis of Gametocyte-Specific Genes in the Rodent Malaria Parasite Plasmodium yoelii [PDF]
, 2016 疟疾是由疟原虫感染导致的蚊媒传染性疾病，每年导致全球上亿人口的感染和上百万人口的死亡，严重危害人类健康。疟原虫具有宿主交替和世代交替的特点，有脊椎动物和按蚊两个宿主，无性生殖和有性生殖两种生殖方式。配子体状态的疟原虫被按蚊吸食后，先后经历雌雄配子体—雌雄配子—合子—动合子—卵囊等发育阶段，这是一个多步骤、多基因调控的复杂过程，是疟疾传播的必须阶段。迄今为止，关于疟原虫配子体阶段特异表达基因的研究相对较少，本研究将重点分析约氏疟原虫配子体阶段特异表达基因的生物学功能 ...
凌渊
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Veterinary Dermatology, Volume 36, Issue 3, Page 302-313, June 2025.Background – Malassezia pachydermatis (MP) is implicated in severe dermatitis and otitis externa (OE) of companion animals and recently gained attention for its increasing resistance to azole compounds. For this reason, developing novel therapeutic strategies is of great interest. In a previous work, we used reference yeast isolates to evaluate several
Costanza Spadini +11 more
wiley +1 more source
长链非编码RNA结肠癌相关转录物2(CCAT2)在膀胱癌中的表达及作用机制
Zhongguo shiyan zhenduanxue, 2020 目的探讨长链非编码RNA(lncRNA)结肠癌相关转录物2(CCAT2)在膀胱癌中的表达及作用机制。方法选取膀胱癌组织及癌旁正常组织、膀胱癌细胞(EJ、BIU87、T24、T24-ADM)及膀胱正常上皮细胞(sv-HUC-1),采用qRT-PCR检测CCAT2表达。选取膀胱癌细胞,分别转染siRNA-NC和siRNA-CCAT2,采用qRT-PCR检测细胞中CCAT2表达;采用CCK8法检测细胞增殖活性;流式细胞仪检测细胞凋亡;划痕实验检测细胞迁移;Western blot法检测凋亡相关蛋白表达。结果 ...
刘明 +6 more
doaj
Transcriptional coactivator SRC-3 plays a protective role in host defense against bacteria-induced colitis and study of mechanism [PDF]
, 2015 类固醇激素受体辅激活子3（Steroidreceptorcoactivator,SRC-3）是一种转录协同因子，它能够与多种核受体及其它转录因子相互作用而增强它们的转录活性。我们以前的研究表明由于SRC-3敲除导致过度的炎症反应和巨噬细胞清除细菌能力的损伤，SRC-3-/-小鼠对大肠杆菌诱导的腹膜炎更敏感，说明SRC-3在宿主防御细菌感染中起着重要作用。最近，我们发现SRC-3在结肠上皮细胞中特异性高表达，并且在小鼠感染Citrobacterrodentium后小鼠结肠中SRC-3蛋白量显著上调 ...
陈文博
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Threats posed by land use to water quality in South Africa's strategic water source areas
World Water Policy, Volume 11, Issue 1, Page 155-164, February 2025.Abstract Understanding the negative impacts of land use/land cover (LULC) on water quality is key to developing effective integrated catchment management strategies. However, in line with the Integrated Water Resources Management (IWRM) philosophy, this necessitates the generation and application of useful scientific knowledge, which stakeholders and ...
Kent Anson Locke, Kevin Winter
wiley +1 more source
p21WAF1/GIP1基因转染人宫颈癌Hela细胞对顺铂敏感性的影响
Zhongguo shiyan zhenduanxue, 2006 目的探讨转染p21WAF1/CIP1基因（p21基因）,对宫颈癌Hela细胞生长抑制作用及p21基因转染Hela细胞对顺铂敏感性的影响。方法应用脂质体转染技术,将质粒pcDNA3转入人宫颈癌Hela细胞中,经G418筛选,转染阳性克隆细胞连续传代培养,并采用中性红摄入法测定顺铂对p21基因转染人宫颈癌Hela细胞增殖抑制作用,流式细胞仪（FCM）检测细胞周期变化。结果经G418筛选,Hela细胞全部死亡,转染质粒pcDNA3的Hela细胞存活,并可连续传代。p21基因转染后,细胞生长明显受到抑制 ...
曹岩, 黄颖, 郑永晨
doaj
miRNA-17~92 Inhibits Colorectal Cancer Progression by Targeting Angiogenesis [PDF]
, 2015 miRNA中的miR-17~92簇在包括结直肠癌（Colorectalcancer，CRC）等人类癌症中广泛表达。已有的研究表明miR-17~92在某些肿瘤模型中可促进肿瘤发生和癌组织血管生成。然而，其在CRC发生和进展中的作用仍然未知。在本研究中，我们意外发现，在用氧化偶氮甲烷（AOM）和右旋糖酐硫酸钠（DSS）诱导的CRC动物模型中，大肠、小肠上皮细胞过表达miR-17~92的转基因小鼠可见肿瘤体积变小、肿瘤血管减少。进一步研究发现，miR-17 ...
潘金水
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