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Rare Metals, Volume 45, Issue 8, August 2026.ABSTRACT Infected bone defects represent a dual therapeutic challenge demanding concurrent infection inhibition and osteogenesis promotion. Systemic antibiotic administration is taken as a classical treatment protocol but often yields inadequate local antibiotic concentrations and delayed bone repair.
Xucai Wang +8 more
wiley +1 more source
, 2009 研究阳离子脂质体在不同细胞中的转染效率及毒性。首先采用DNA延滞实验研究Lipofectamine2000、DOTAP转染试剂与DNA的结合能力。然后选用绿色荧光蛋白的质粒pGFP-N2作为报告基因模型和转染试剂制成复合物后转染Hela、7721、HT29细胞，比较多种因素对转染的影响。最后使用MTT比色法分析Lipofectamine2000、DOTAP转染试剂对细胞的毒性。随着复合物中转染试剂比例的增加，Lipofectamine2000、DOTAP与DNA结合能力逐渐增强 ...
段艳() +7 more
core +1 more source
Recovering Critical Elements From Wastewater for Battery Materials: A Review
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Driven by global carbon neutrality goals, the transition toward a circular economy and sustainable resource management has become increasingly imperative. Given the indispensable role of battery technologies in modern energy storage systems, strategic elements such as lithium (Li), cobalt (Co), and nickel (Ni)—identified by the European ...
Ting Lei +5 more
wiley +1 more source
外源性表达VEGF165b对人膀胱癌裸鼠移植瘤生长影响的研究
Zhongguo shiyan zhenduanxue, 2014 目的建立人膀胱癌T24细胞裸鼠移植瘤模型,探讨外源性表达VEGF165b对其生长的影响及其机制。方法分别移植未转染和转染VEGF165b表达载体的人膀胱癌T24细胞到裸鼠膀胱内,28d使处死动物,称量肿瘤重量,测定肿瘤体积。Western blot法检测肿瘤VEGF165b、p-VEGFR2、AKT、p-AKT蛋白表达。结果转染pcDNA-VEGF165b组肿瘤重量和体积与未转染组和转染pcDNA3.0组相比明显降低（P0.05）。Western blot结果显示,转染pcDNA ...
张金 +4 more
doaj
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT Deep skin infections and dense biofilm barriers caused by Propionibacterium acnes (P. acnes) remain significant challenges in recurrent acne vulgaris, exacerbated by the poor stratum corneum penetration of conventional therapies and rising antibiotic resistance.
Bingran Qi +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2015 目的探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）基因转染大鼠内皮祖细胞的方法,分析转染VEGF基因对内皮祖细胞生长的影响。方法分离大鼠骨髓内皮祖细胞并诱导其分化,采取FITCUEA-I和Dil-Ac-LDL双染色法验证;采用腺病毒包装VEGF165基因,按照不同比例转染内皮祖细胞后,通过荧光显微镜观察质粒自带绿色荧光蛋白验证转染效率;以未转染及转染空载体为对照;采用MTT法验证转染VEGF165基因对内皮祖细胞增殖的影响 ...
王伟 +5 more
doaj
siRNA抑制人卵巢癌SKOV-3细胞MDM2的表达及细胞增殖的研究
Zhongguo shiyan zhenduanxue, 2021 目的研究siRNA对人卵巢癌细胞SKOV-3细胞MDM2基因表达的抑制作用及对肿瘤细胞增殖和凋亡的影响。方法体外合成2对针对MDM2基因的siRNA,经脂质体转染导入SKOV-3细胞;转染siRNA MDM2(简称si-MDM2)转染阴性对照质粒到SKOV-3,并设只加转染试剂作空白对照组,采用MTT法检测si-MDM2对细胞增殖的抑制作用。流式细胞仪检测细胞凋亡,并用Real-time PCR和Western blot分别从mRNA水平和蛋白水平检测si-MDM2对MDM2的抑制作用。结果 ...
王莹, 贾志勇, 赵燕颖
doaj
Rare Metals, Volume 45, Issue 7, July 2026.ABSTRACT The pathogenesis of diabetic foot ulcers is complex, often involving polymicrobial infection and persistent inflammatory responses, which lead to delayed or nonhealing wounds, posing a formidable clinical challenge. Existing photodynamic therapy reagents and traditional treatments generally address only a single pathological aspect, and the ...
Yu Zhang +16 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2012 目的探讨klotho基因转染对脂肪间充质干细胞（ADSCs）增殖的影响。方法将含有klotho全长基因的真核质粒转染至从脂肪组织分离的ADSCs中,采用CCK-8法测定转染后细胞增殖率,western blot法检测转染后细胞周期蛋白D1的表达变化,流式细胞术检测转染前后ADSCs的细胞周期变化。结果转染klotho基因96h后AD-SCs的细胞增殖率为170.50%;流式细胞术检测结果显示,ADSCs转染klotho基因S期和G2期细胞明显增多;west-ern ...
韩冬, 徐煌, 郑永霞, 肖燕萍
doaj
Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT Cross‐scale assembly of metal (sub) nanoparticles and/or single atoms into millimeter/nanostructured metal monolithic catalysts (Min‐SMCs) is highly appealing for sustainable water cleanup, due to energy‐efficient catalyst recyclability, high infrastructure compatibility, and low metal‐releasing risks.
Jia‐Cheng E. Yang +4 more
wiley +1 more source