Results 21 to 30 of about 17,301 (184)
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨蛋白酶体筇亚单位（PSMB5）基因过表达对氧化条件下晶状体上皮细胞的影响。【方法】构建重组质粒pcDNA3．1-PSMB5并将其转染人人晶状体上皮细胞株SRAO1／04中，形成稳定表达，同时设pcDNA3．1空载体为对照组。RT—PCR法及Western blot法分别检测PSMB5基因及蛋白的表达情况。H2O2分别作用PSMB5转染细胞及空载体转染细胞，MTT法检测细胞增殖能力的变化。【结果】转染后用G418筛选3周后，获得PSMB5转染及空载体转染的G418抗性的细胞克隆 ...
张铁英 +3 more
doaj
Rare Metals, Volume 45, Issue 1, January 2026.ABSTRACT The electrocatalytic reduction of nitrate (NO3ER) has emerged as a promising strategy for mitigating NO3− ${{\text{NO}}_{3}}^{-}$ pollution in wastewater while concurrently producing value‐added ammonia; however, its process is significantly hindered by the lack of highly efficient and low‐cost electrocatalysts. Herein, we proposed several non‐
Xue‐Shi Song +5 more
wiley +1 more source
外源性表达VEGF165b对人膀胱癌裸鼠移植瘤生长影响的研究
Zhongguo shiyan zhenduanxue, 2014 目的建立人膀胱癌T24细胞裸鼠移植瘤模型,探讨外源性表达VEGF165b对其生长的影响及其机制。方法分别移植未转染和转染VEGF165b表达载体的人膀胱癌T24细胞到裸鼠膀胱内,28d使处死动物,称量肿瘤重量,测定肿瘤体积。Western blot法检测肿瘤VEGF165b、p-VEGFR2、AKT、p-AKT蛋白表达。结果转染pcDNA-VEGF165b组肿瘤重量和体积与未转染组和转染pcDNA3.0组相比明显降低（P0.05）。Western blot结果显示,转染pcDNA ...
张金 +4 more
doaj
TIPE2 promote synovial fibroblasts cell apoptosis of adjuvant arthritis rats by up-regulating caspase while down-regulating NF-κB [PDF]
, 2015 目的：TIPE2能够抑制炎症以及肿瘤的发生发展，并且TIPE2过表达能够促进细胞的凋亡。类风湿关节炎是一种免疫多态性慢性疾病，我们应用类风湿关节炎模型大鼠佐剂型关节炎滑膜成纤维样细胞(fibroblast-likesynoviocytesofratadjuvantarthritis，AA-FLS)，通过体外实验分析TIPE2对死亡受体5诱导细胞凋亡的作用探讨TIPE2对佐剂型关节炎发病的影响。 方法：通过RT-PCR、荧光实时定量PCR和Western ...
石春燕
core
The Genome of Phoebe zhennan (Lauraceae) and Comparative Genomics of Phoebe 楠木(樟科)基因组与楠属植物比较基因组学研究
Integrative Conservation, Volume 4, Issue 4, Page 584-599, December 2025.Phoebe zhennan is a Chinese endemic and valuable timber species of Phoebe (Lauraceae). We present a chromosome‐level genome assembly of P. zhennan. Our research provides novel insights into the genome evolution of Phoebe, including whole‐genome duplication, phylogenetic relationships of magnoliids, and key MYB regulatory genes involved in lignin ...
Mao Yameng +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2015 目的探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）基因转染大鼠内皮祖细胞的方法,分析转染VEGF基因对内皮祖细胞生长的影响。方法分离大鼠骨髓内皮祖细胞并诱导其分化,采取FITCUEA-I和Dil-Ac-LDL双染色法验证;采用腺病毒包装VEGF165基因,按照不同比例转染内皮祖细胞后,通过荧光显微镜观察质粒自带绿色荧光蛋白验证转染效率;以未转染及转染空载体为对照;采用MTT法验证转染VEGF165基因对内皮祖细胞增殖的影响 ...
王伟 +5 more
doaj
The structure and function of WBP2 in Glioma [PDF]
, 2016 脑白质疏松症(Leukoariaosis，LA)在中年及老年人中逐渐普遍，随着年龄的 增长，患者表现出认知功能障碍、运动功能障碍、痴呆、抑郁和中风等，这在很 大程度上加重了公共医疗的负担。但是目前人们对脑白质疏松症的病理生理机制 所知甚少。 分子生物学的飞速发展为科学家们研究疾病的致病基因及发病机制提供了 更好的条件和更广阔的前景。文献报道WBP2基因SNP位点的突变与LA的发 病相关。研究发现WBP2也参与了乳腺癌的发生，因此，推测WBP2与神经疾 病和肿瘤的发生均密切相关 ...
张亚
core
Integrative Conservation, Volume 4, Issue 4, Page 527-533, December 2025.The recommendations cover four key aspects namely the definition of the taxa require high priority in the biodiversity conservation management, enhanced flexibility and adaptivity of the implementation processes, enforced integration of research advancement to enable the sustainable usage of plant resources, and finally actions to propose transboundary
Shi‐Cheng Shao +4 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2012 目的探讨klotho基因转染对脂肪间充质干细胞（ADSCs）增殖的影响。方法将含有klotho全长基因的真核质粒转染至从脂肪组织分离的ADSCs中,采用CCK-8法测定转染后细胞增殖率,western blot法检测转染后细胞周期蛋白D1的表达变化,流式细胞术检测转染前后ADSCs的细胞周期变化。结果转染klotho基因96h后AD-SCs的细胞增殖率为170.50%;流式细胞术检测结果显示,ADSCs转染klotho基因S期和G2期细胞明显增多;west-ern ...
韩冬, 徐煌, 郑永霞, 肖燕萍
doaj
Comparative Proteomics Study on Human High-metastatic Large Cell Lung Cancer Cell Lines Before and After Transfecting with nm23-H1 Gene [PDF]
, 2010 Background and objective As a tumor metastasis suppressor gene, the functions of nm23-H1 gene are still unclear. The aim of this study is to better understand the mechanism of lung cancer metastasis and to find new biomarkers for early diagnosis and new ...
Li MA +6 more
core