Results 31 to 40 of about 127,482 (174)
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨蛋白酶体筇亚单位（PSMB5）基因过表达对氧化条件下晶状体上皮细胞的影响。【方法】构建重组质粒pcDNA3．1-PSMB5并将其转染人人晶状体上皮细胞株SRAO1／04中，形成稳定表达，同时设pcDNA3．1空载体为对照组。RT—PCR法及Western blot法分别检测PSMB5基因及蛋白的表达情况。H2O2分别作用PSMB5转染细胞及空载体转染细胞，MTT法检测细胞增殖能力的变化。【结果】转染后用G418筛选3周后，获得PSMB5转染及空载体转染的G418抗性的细胞克隆 ...
张铁英 +3 more
doaj
Rare Metals, Volume 45, Issue 3, March 2026.ABSTRACT High‐entropy alloys (HEAs) have attracted considerable interest from researchers owing to their tunable chemical compositions, exceptional structural stability, and promising catalytic properties. However, their large‐scale application is often hindered by complex manufacturing techniques and poor durability.
Sheng Guo +9 more
wiley +1 more source
, 2009 研究阳离子脂质体在不同细胞中的转染效率及毒性。首先采用DNA延滞实验研究Lipofectamine2000、DOTAP转染试剂与DNA的结合能力。然后选用绿色荧光蛋白的质粒pGFP-N2作为报告基因模型和转染试剂制成复合物后转染Hela、7721、HT29细胞，比较多种因素对转染的影响。最后使用MTT比色法分析Lipofectamine2000、DOTAP转染试剂对细胞的毒性。随着复合物中转染试剂比例的增加，Lipofectamine2000、DOTAP与DNA结合能力逐渐增强 ...
段艳() +7 more
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Rare Metals, Volume 45, Issue 3, March 2026.ABSTRACT Against the backdrop of global clean energy transition and surging demand for lithium resources, lithium extraction from salt lakes presents unprecedented development opportunities. Nanofiltration (NF) membrane technology, recognized for its efficiency, energy savings, and environmental benefits, demonstrates significant application potential ...
Wen‐Feng Li +5 more
wiley +1 more source
外源性表达VEGF165b对人膀胱癌裸鼠移植瘤生长影响的研究
Zhongguo shiyan zhenduanxue, 2014 目的建立人膀胱癌T24细胞裸鼠移植瘤模型,探讨外源性表达VEGF165b对其生长的影响及其机制。方法分别移植未转染和转染VEGF165b表达载体的人膀胱癌T24细胞到裸鼠膀胱内,28d使处死动物,称量肿瘤重量,测定肿瘤体积。Western blot法检测肿瘤VEGF165b、p-VEGFR2、AKT、p-AKT蛋白表达。结果转染pcDNA-VEGF165b组肿瘤重量和体积与未转染组和转染pcDNA3.0组相比明显降低（P0.05）。Western blot结果显示,转染pcDNA ...
张金 +4 more
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Ultrasound in Obstetrics &Gynecology, Volume 67, Issue 3, Page 376-384, March 2026.ABSTRACT Objective To investigate whether combining abnormal morphological features observed on ultrasound in live pregnancies that ended in a first‐trimester miscarriage can predict an abnormal karyotype. Methods This retrospective observational cohort study was conducted at the early‐pregnancy assessment unit at University College London Hospital ...
T. Setty +5 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2015 目的探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）基因转染大鼠内皮祖细胞的方法,分析转染VEGF基因对内皮祖细胞生长的影响。方法分离大鼠骨髓内皮祖细胞并诱导其分化,采取FITCUEA-I和Dil-Ac-LDL双染色法验证;采用腺病毒包装VEGF165基因,按照不同比例转染内皮祖细胞后,通过荧光显微镜观察质粒自带绿色荧光蛋白验证转染效率;以未转染及转染空载体为对照;采用MTT法验证转染VEGF165基因对内皮祖细胞增殖的影响 ...
王伟 +5 more
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Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT Photocatalytic molecular oxygen (O2) activation provides a sustainable approach to produce singlet oxygen (1O2) for organic contaminants detoxification. However, the charge carriers‐involved pathway usually suffers from unsatisfactory 1O2 production efficiency owing to energy loss caused by photogenerated hole‐mediated superoxide species (•O2−
Yan‐Biao Shi +8 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2012 目的探讨klotho基因转染对脂肪间充质干细胞（ADSCs）增殖的影响。方法将含有klotho全长基因的真核质粒转染至从脂肪组织分离的ADSCs中,采用CCK-8法测定转染后细胞增殖率,western blot法检测转染后细胞周期蛋白D1的表达变化,流式细胞术检测转染前后ADSCs的细胞周期变化。结果转染klotho基因96h后AD-SCs的细胞增殖率为170.50%;流式细胞术检测结果显示,ADSCs转染klotho基因S期和G2期细胞明显增多;west-ern ...
韩冬, 徐煌, 郑永霞, 肖燕萍
doaj
Evolution of research on global amphibian declines
Conservation Biology, Volume 40, Issue 1, February 2026.Abstract In the late 1980s, the scientific community became aware of severe, enigmatic amphibian population declines. These declines triggered a wave of research focused on quantifying their extent and identifying key drivers. We used text‐analysis techniques, including topic modeling and geoparsing, to examine the evolution of research focused on ...
Jordann Crawford‐Ash +6 more
wiley +1 more source