Results 41 to 50 of about 69,546 (177)
Focusing Events, Agenda Setting, and Narrative Numbing
Review of Policy Research, EarlyView.ABSTRACT Focusing events are important in understanding policy change, as demonstrated in Birkland's work on agenda setting, the multiple streams framework, and the narrative policy framework. These frameworks emphasize the role of focusing events in drawing public attention to broader issues.
Megan K. Warnement Wrobel +1 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的探讨β连环素（β-catenin）和MMP-2在非小细胞肺癌中的表达及意义。方法采用免疫组织化学方法检测39例肺癌组织及8例正常对照肺组织中β-catenin和MMP-2的表达,并结合临床和病理资料进行分析。结果 1.β-catenin和MMP-2在非小细胞肺癌组织中的胞质表达明显高于对照组,差异具有显著性 ...
孔康宝, 李光虎, 杨波, 崔永生
doaj
MicroRNA-186 inhibits prostate cancer cell proliferation and tumor growth by targeting YY1 and CDK6 [PDF]
, 2016 目的 目前的研究已经证实miRNA186在包括非小细胞肺癌、膀胱癌、胰腺导管癌等肿瘤中起着重要的调控作用，但是miRNA186与前列腺癌之间的关系目前还没有研究。本研究的目的主要是探究miRNA186对前列腺癌细胞生物学功能的影响，寻找miRNA186在前列腺癌细胞中作用的下游靶基因，探究其作用的机制。 方法 首先通过荧光定量PCR的方法检测miRNA186在前列腺上皮细胞和3种不同前列腺癌细胞之间的表达情况，然后构建高表达miRNA186的重组质粒载体 ...
陆树
core
Veterinary Dermatology, EarlyView.ABSTRACT Background Canine atopic dermatitis (cAD) is a multifactorial, inherited skin disease, estimated to affect ≤ 15% of dogs. Studies of skin messenger mRNA in cAD currently use invasive methods, including blood sampling and biopsy collection, whilst advances in human atopic dermatitis study methodology have demonstrated reliable use of minimally ...
Xavier Langon +5 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2012 目的探讨Survivin和Ang2在人乳腺癌组织中的表达及其在淋巴转移中的作用。方法2060例乳腺癌组织,应用免疫组织化学S-P法检测Survivin和Ang2在乳腺癌组织中的表达情况,RT-PCR检测Survivin及VEGF-CmRNA的表达。结果2060例乳腺癌组织中Survivin和Ang2的表达阳性率分别为85.0%和73.3%,Survivin阳性表达组中Ang2的表达为74.5%（35/51）,Survivin阴性表达组中Ang2的表达为66.7%（6/9）,经直线相关分析 ...
李湘奇
doaj
comparative analysis of Litopenaeus vannanmei by transcriptome sequencing and characterization of two immune-relevant genes [PDF]
, 2015 南美白对虾是目前对虾养殖的主要品种，具有巨大的经济价值。然而，病害的发生严重危害其养殖业的发展，研究对虾的先天免疫反应能够给对虾养殖中病害的防治提供重要的理论基础。 转录组测序能够分析某一生理条件下细胞内所有mRNA的表达水平，本研究通过二代测序技术首次比较分析了对WSSV（whitespotsyndromevirus）抗性差异的两种南美白对虾群体的转录组，它们的基因表达水平存在明显差异，具有750个明显差异表达的基因，普通对虾及抗病对虾群体在病毒感染前后分别有479和132个差异表达基因 ...
徐景祥
core
Veterinary Dermatology, Volume 37, Issue 2, Page 200-210, April 2026.Background: Antimicrobial stewardship has become vital given the progressive emergence of multidrug‐resistant bacteria, and novel approaches to the treatment of bacterial infections are needed. Recently, reported synergistic effects of antibacterial drugs and bacteriophage therapy have revealed promising applications for the management of meticillin ...
Sarah Ehling +9 more
wiley +1 more source
Survivin和Caspas3在侧向发育型大肠肿瘤中的表达
Zhongguo shiyan zhenduanxue, 2011 目的研究凋亡相关基因Survivin和Caspase3蛋白在侧向发育型大肠肿瘤（LST）中的表达。方法应用免疫组织化学方法检测良性LST、大肠腺瘤、大肠腺癌及其正常黏膜组织中Survivin和Caspase3的蛋白表达。结果 Sur-vivin在大肠腺癌（61.9%）高表达,良性LST（21.7%）和大肠腺瘤（20.6%）表达降低,而在正常黏膜组织不表达。Cas-pase3在正常黏膜高表达（90.9%）,在良性LST（60.9%）和大肠腺瘤（64.7%）表达降低,在大肠腺癌（42.9%）低表达 ...
吴晓冬 +5 more
doaj
A Study on Roles of Slit2 in Hepatocellular Carcinoma Cells [PDF]
, 2015 Slit家族蛋白是一种进化上高度保守的细胞外分泌糖蛋白。对Slit的研究最初的重点是它在神经生长的作用，Slit作为配体通过与跨膜受体Roundabout(Robo)结合在中枢神经系统轴突导向中起到重要的作用。近些年的研究发现Slits与Robos在一些肿瘤中异常表达，说明Slit/Robo信号在肿瘤的发生发展过程中起到重要的作用。 Oncomine数据库的检索结果显示，与正常组织相比，肝细胞癌中受体Robo1的表达量有显著的增加，而Slit2的表达量则有一定的差别，多数情况下表现为下调 ...
朱韦林
core
Rare Metals, Volume 45, Issue 3, March 2026.ABSTRACT Cost‐effective and efficient non‐noble‐metal catalysts for selective lignin hydrogenolysis remain elusive, representing a significant challenge in biorefinery. Here, we prepared a nitrogen‐doped carbon supported CoNi bimetallic catalyst, which was successfully derived from zeolitic imidazolate framework (ZIF) via high‐temperature pyrolysis ...
Xingwei Luo +3 more
wiley +1 more source