Zhongshan Daxue xuebao. Yixue kexue ban, 2015 摘 要: 【目的】 探讨AKT不同亚型AKT1和AKT2在成人脂肪干细胞成脂分化过程的表达及意义?【方法】取生长状态良好的P3代的成人脂肪组织来源的间充质干细胞(hadsc),以负载AKT1-shrna和AKT2-SHRNA的慢病毒载体分别转染hadsc,获得稳定干扰AKT1和AKT2的细胞株(分别设为AKT1-3组和AKT2-1组),转染非干扰序列NTC-shrna的设为对照组?将上述细胞分别进行成脂肪诱导,观察脂滴的形成,并检测相关成脂肪基因的表达和成脂肪关键转录因子pparγ的表达?
doaj
Anti-inflammatory effects and related mechanisms of naringenin in human periodontal ligament stem cells under lipopolysaccharide stimulation based on RNA sequencing. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiLi J +5 more
europepmc +1 more source
[Detection of molecular affecting sensitivity to local glucocorticoid therapy in oral lichen planus through transcriptome sequencing]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanRen X, Li K, Li C.
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[Clinical characteristics of DUX4-IGH fusion B-cell acute lymphoblastic leukemia in children]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiLuo YX +4 more
europepmc +1 more source
Zhongguo shiyan zhenduanxue目的应用单细胞测序技术探索阿尔茨海默病的发病机制。方法从单细胞转录组数据库下载疾病组及对照组数据，下载后进行数据质控，利用Seurat软件进行数据整合、聚类，利用T-分布随机邻域嵌入和一致流形逼近与投影软件进行图的可视化。利用SingleR与人工检查的综合方式完成细胞注释，最后将进一步进行基因差异分析、拟时序分析、细胞间通讯分析。结果细胞图谱构建结果显示：疾病组细胞类型为中性粒细胞、小胶质细胞、神经元以及星形胶质细胞。对照组细胞类型为神经元与星形胶质细胞；基因表达差异结果显示：CD74、B2M、SPP1、
任彦学 +6 more
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[Progress of scRNA-seq technology in nasopharyngeal carcinoma research]. [PDF]
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za ZhiZheng B, Jin G.
europepmc +1 more source
[High-throughput circular RNA sequencing reveals tumor-specific high expression of hsa_circ_0001900 in Wilms tumor in association with poor prognosis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoGao Z +9 more
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 【目的】 选用高成瘤性前列腺癌细胞亚克隆细胞株LNCap1-luc构建人类前列腺癌裸鼠移植瘤模型,应用于反基因治疗,探讨该细胞株进行抗肿瘤研究的价值?【方法】 采用皮下接种法制备24只荷LNCap1-luc前列腺癌移植瘤裸鼠模型,随机分为3组:三螺旋形成寡核苷酸(TFO)治疗组?序列对照寡核苷酸(SCO)非特异性对照组?生理盐水(NS)阴性对照组?瘤内注射给药6周,测量移植瘤质量和体积,计算抑瘤率?实时荧光定量逆转录PCR(qRT-PCR)检测肿瘤组织AR mRNA表达;免疫组化法检测AR,PSA ...
doaj
[Effects of miR-100-5p on proliferation and apoptosis of acute myeloid leukemia cells and the underlying molecular mechanism]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiFan XR, Yang Y, Sun Y, DU CM.
europepmc +1 more source
[PINK1 suppresses colorectal cancer cell growth through epigenetic regulation of histone modifications]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanWang M, Luan S, Fan X, Han D, Zhu Y.
europepmc +1 more source