Results 1 to 10 of about 114,725 (118)

基于RNA-Seq技术的野生和圈养塔里木马鹿皮肤组织转录组比较分析

open access: yes, 2023
为探寻野生和圈养塔里木马鹿(Cervus elaphus yarkandensis)的基因表达谱是否存在差异,并进一步筛选该物种适应干旱环境的相关基因,利用RNA-Seq技术对野生和圈养塔里木马鹿的皮肤组织进行转录组测序,对测序数据质控后比对参考基因组,筛选差异表达基因并对其进行功能注释和富集分析,利用qRT-PCR方法对转录组测序结果进行可靠性检测。结果显示:与圈养塔里木马鹿相比,在野生塔里木马鹿皮肤组织中共有3 303个差异表达基因,包括上调表达基因1 535个,下调表达基因1 768个 ...
单文娟   +3 more
core   +1 more source

Cross‐stage single‐cell and spatial metabolome analyses reveal periderm specialization and tanshinone biosynthesis in Salvia miltiorrhiza roots

open access: yesNew Phytologist, EarlyView.
Biosynthesis and accumulation of tanshinones in the periderm of Salvia miltiorrhiza during secondary root growth. Summary Perennial herbs develop long‐lived roots that undergo repeated cycles of secondary growth, during which the periderm functions as a protective barrier and a major site of specialized metabolite accumulation.
Licheng Liu   +19 more
wiley   +1 more source

3D Printed High‐Strength Hydrogel for Dual Release of Cu2+ and Ag+ Ions to Enhance Bone Repair and Antibacterial Properties

open access: yesRare Metals, Volume 45, Issue 6, June 2026.
ABSTRACT Bone restoration is often hindered by the spatiotemporal dysregulation of immune and regenerative signals. Conventional bio‐inert implants, providing only passive support, fail to modulate this microenvironment, leading to infections and poor healing.
Zhizhong Sun   +8 more
wiley   +1 more source

Assessing D‐Squame as a Minimally Invasive Technique to Evaluate the Cutaneous Immune Response mRNA in a Dog Model of Canine Atopic Dermatitis

open access: yesVeterinary Dermatology, Volume 37, Issue 3, Page 353-364, June 2026.
ABSTRACT Background Canine atopic dermatitis (cAD) is a multifactorial, inherited skin disease, estimated to affect ≤ 15% of dogs. Studies of skin messenger mRNA in cAD currently use invasive methods, including blood sampling and biopsy collection, whilst advances in human atopic dermatitis study methodology have demonstrated reliable use of minimally ...
Xavier Langon   +5 more
wiley   +1 more source

Tolerability and Feasibility of Minimally Invasive Canine Skin Sampling: Excellent Tolerability Meets Transcriptomic Challenges

open access: yesVeterinary Dermatology, Volume 37, Issue 3, Page 375-385, June 2026.
ABSTRACT Background Advances in transcriptomics have driven the demand for minimally invasive, reproducible and high‐yield skin sampling methods, particularly for studying inflammatory skin diseases in companion animals. Hypothesis/Objectives We tested tolerability, feasibility and RNA quantity and quality of three minimally invasive skin sampling ...
Ina Herrmann   +2 more
wiley   +1 more source

Developing HMME‐Conjugated NIR‐II Activated Carbon Dots Nanospray for the Treatment of MRSA Biofilm Infections in Diabetic Wound via PTT/CDT/SDT

open access: yesRare Metals, Volume 45, Issue 4, April 2026.
ABSTRACT Diabetic wound infections are a common complication of diabetes, which severely impact patients' quality of life. Effective treatment of diabetic wound infections remains one major challenge in the clinic, partially due to the formation of bacterial biofilm and antibiotic resistance.
Liang Zhou   +8 more
wiley   +1 more source

Automating the analysis of public saliency and attitudes toward biodiversity from digital media

open access: yesConservation Biology, Volume 40, Issue 2, April 2026.
Abstract Measuring public attitudes toward wildlife provides crucial insights into human relationships with nature and helps monitor progress toward Global Biodiversity Framework targets. Yet, conducting such assessments at a global scale presents challenges.
Noah Giebink   +8 more
wiley   +1 more source

Mountains Without Borders: Scaling up Conservation Success for Snow Leopards

open access: yesIntegrative Conservation, Volume 5, Issue 1, Page 1-4, March 2026.
This special issue assembles pioneering research addressing three critical and interconnected challenges: establishing where viable populations persist at range edges, advancing methods to monitor this cryptic species and its prey effectively, and evaluating whether landscapes remain connected across political boundaries.
Juan Li, Lingyun Xiao
wiley   +1 more source

EB病毒基因在鼻咽癌组织转录表达的研究 [PDF]

open access: yes, 1993
本研究在转录水平上,利用转录载体PGEM-2与两种EB病毒基因片段(LMP和FBER-1)分别构建重组质粒,以SP~6和T~7RNA聚合酶合成含有~35标记的单链反义RNA(Anti-sense RNA或cRNA)和正义RNA(sense RNA),并分别与经病理确诊的鼻咽癌组织40例进行组织原位杂交,结果显示与40例鼻咽癌组织原位杂交后,LMP单链反义RNA有36例呈阳性,其阳性率为90%;EBER-1反义RNA40例均呈阳性,阳性率为100 ...

core  

Bridging the Digital Divide: Examining the Role of Digital Health in Nepal's Healthcare Transformation

open access: yesWorld Medical &Health Policy, Volume 18, Issue 1, March 2026.
ABSTRACT This systematic review examines the digital health interventions for inclusive and equitable solutions for transforming healthcare access and delivery in Nepal. The research focuses on improving digital health accessibility among marginalized and rural populations of Nepal.
Manoj Kumar Dahal, Gary L. Kreps
wiley   +1 more source

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