Results 21 to 30 of about 11,516 (168)
Automating the analysis of public saliency and attitudes toward biodiversity from digital media
Conservation Biology, Volume 40, Issue 2, April 2026.Abstract Measuring public attitudes toward wildlife provides crucial insights into human relationships with nature and helps monitor progress toward Global Biodiversity Framework targets. Yet, conducting such assessments at a global scale presents challenges.
Noah Giebink +8 more
wiley +1 more source
The role and mechanism of hnRNP A2/B1 in breast cancer [PDF]
, 2015 核不均一核糖核蛋白A2/B1（hnRNPA2/B1）是hnRNP蛋白家族成员，在肿瘤细胞增殖与凋亡过程中具有重要作用。本实验为了探究hnRNPA2/B1蛋白在乳腺癌组织中的表达及其在乳腺癌细胞中的定位。研究hnRNPA2/B1在乳腺癌细胞中所涉及的主要功能、信号通路以及可变剪接和蛋白融合过程，并探究hnRNPA2/B1对细胞增殖、凋亡、迁移等生物功能的影响。从而阐明hnRNPA2/B1在乳腺癌中的作用机制，为肿瘤的机理研究、临床诊断和治疗提供一个新靶点。本实验用免疫组化方法检测hnRNPA2 ...
韩荣
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Mountains Without Borders: Scaling up Conservation Success for Snow Leopards
Integrative Conservation, Volume 5, Issue 1, Page 1-4, March 2026.This special issue assembles pioneering research addressing three critical and interconnected challenges: establishing where viable populations persist at range edges, advancing methods to monitor this cryptic species and its prey effectively, and evaluating whether landscapes remain connected across political boundaries.
Juan Li, Lingyun Xiao
wiley +1 more source
霍乱弧菌新发菌株中
Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的探索霍乱弧菌新发菌株H1的六型分泌系统（T6SS）存在的转录调控机制。方法本研究以转录组测序为基础，分析H1株分别由于vipA敲除（H1ΔvipA），vipB敲除（H1ΔvipB）和clpV敲除（H1ΔclpV）时T6SS基因簇的转录水平，并对其差异基因进行了基因本体论（GO）富集和京都基因与基因组百科全书（KEGG）富集。结果发现H1ΔvipA有22个差异基因（FDR<0.01，|log2FC|≥1），其中有18个属于T6SS基因簇；并且除vipA外，其转录水平均呈上升趋势 ...
吴文轩, 郭扬, 张幸鼎, 赵文婧
doaj
Studies on Heat Tolerance in Abalone Based on the Measurement of Heart Rate [PDF]
, 2016 鲍是海产八珍之首，2015年，我国的鲍产量已经超过13万吨，其中福建省的产量占全国的80%。皱纹盘鲍为温带种，原分布于我国黄、勃海海域，1990年代后期南移福建后，获得了极大发展，由此福建逐步成为我国鲍养殖的主产区。但福建海域的夏季高水温往往导致鲍的高死亡率，这一直困扰着鲍养殖产业的发展。因此，培育耐高温的养殖新品种显得格外重要，而其中建立鲍的耐高温性状的准确测评方法是品种培育的关键。本研究中，我们通过测定阿氏拐点温度（Arrheniusbreakpointtemperature，ABT ...
陈楠
core
World Medical &Health Policy, Volume 18, Issue 1, March 2026.ABSTRACT This systematic review examines the digital health interventions for inclusive and equitable solutions for transforming healthcare access and delivery in Nepal. The research focuses on improving digital health accessibility among marginalized and rural populations of Nepal.
Manoj Kumar Dahal, Gary L. Kreps
wiley +1 more source
正常乳腺组织和浸润性乳腺癌组织中淋巴增强因子-1（LEF-1）转录因子的蛋白质表达水平的分析
Zhongguo shiyan zhenduanxue, 2010 目的通过实验对正常乳腺组织和浸润性乳腺癌组织中淋巴增强因子-1（LEF-1）转录因子的蛋白质表达水平进行分析,观察其差异。方法对从手术获得的新鲜乳腺组织做冰冻切片,经H&E染色分析鉴定出正常乳腺组织和乳腺浸润癌组织。用显微切割方法获取目标组织,再采用蛋白质免疫印迹法（Westen blot）检测正常乳腺组织和乳腺浸润癌组织中LEF-1转录因子的蛋白质表达差异。结果乳腺浸润癌组织中的LEF-1转录因子的蛋白质表达水平高于正常乳腺组织中的LEF-1转录因子的蛋白质表达水平。结论LEF ...
盖晋宏 +5 more
doaj
Separation and Activity Screening of Peptides Derived from Mycale phyllophila [PDF]
, 2015 海绵是最原始的多细胞生物，拥有许多在结构上非常多样化的生物活性物质，是最具有药物开发潜力的海洋生物资源。这些生理活性物质常具有抗肿瘤、抗病毒、抗细菌、抗真菌、杀虫、抗炎、神经抑制、神经保护、防污等作用。作为海绵活性物质中重要的组成部分，海绵活性多肽具有分子量小、结构简单、生物效应多样、免疫原性低等特点，具良好的开发潜力。叶片山海绵（Mycalephyllophila）是福建沿海资源较丰富的物种，本研究以叶片山海绵为材料，建立海绵水溶性肽类的分离纯化、活性筛选和结构测定的方法 ...
贺腾飞
core
SWEET1‐mediated glucose transport is crucial for energy availability in Arabidopsis
New Phytologist, Volume 249, Issue 4, Page 1816-1830, February 2026.Summary The stress‐responsive hormone abscisic acid (ABA) is known for its inhibitory effects on various physiological processes, including seed germination, often resulting in energy deprivation. Interestingly, ABA‐induced germination inhibition can be alleviated by exogenous glucose (Glc), mimicking a functional interplay between ABA and sugar ...
Xueyi Xue +3 more
wiley +1 more source
pSiRNA-Shh逆转录病毒载体的构建及对恶性脑胶质瘤细胞作用的离体实验研究
Zhongguo shiyan zhenduanxue, 2007 目的建立逆转录病毒介导的人Shh基因RNA干扰体外表达体系并观察对恶性脑胶质瘤细胞的作用。方法将人Shh基因RNA干扰双链转录DNA片段重组到逆转录病毒质粒Psilencer 5.1-H1 Retro中,构建成携带人Shh基因RNA干扰逆转录病毒载体pSiRNA-Shh,经PT67细胞包装后,产生的重组逆转录病毒感染恶性脑胶质瘤细胞株U251和CHG-5细胞,用WST-8、RT-PCR和Western Blotting分别检测对转染细胞活性、人Shh mRNA和蛋白表达的影响。结果重组pSiRNA ...
尹昌林 +5 more
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