Results 11 to 20 of about 114,725 (118)
构建人CD23及其反义RNA的逆转录病毒表达重组体 [PDF]
, 1996 利用ＣＤ２３ｃＤＮＡ全基因克隆ｐＵＣＤ９７６，分别以限制性内切酶ＥｃｏＲＩ和ＸｂａＩ酶切该重组质粒ＤＮＡ，回收１．０ｋｂ的ＣＤ２３ｃＤＮＡ全基因、ＨｉｎｄⅢ酶切后回收３’端６０７ｂｐ的结合区段基因以及ＥｃｏＲⅠ和ＨｉｎｄⅡ酶切回收５’端４０３ｂｐ的调控区段基因。将各回收片段先以Ｋｌｅｎｏｗ酶补平后，插入已补平的ｐＺＩＰ逆转录病毒载体ＢａｍＨⅠ单切点，利用地高辛素标记的ＣＤ２３ｃＤＮＡ全基因探针，以斑点核酸杂交筛选逆转录病毒重组体，结合用限制性内切酶ＢａｍＨＩ和／或ＢｇｌⅡ酶切鉴定插入方向，最终获得正 ...
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SWEET1‐mediated glucose transport is crucial for energy availability in Arabidopsis
New Phytologist, Volume 249, Issue 4, Page 1816-1830, February 2026.Summary The stress‐responsive hormone abscisic acid (ABA) is known for its inhibitory effects on various physiological processes, including seed germination, often resulting in energy deprivation. Interestingly, ABA‐induced germination inhibition can be alleviated by exogenous glucose (Glc), mimicking a functional interplay between ABA and sugar ...
Xueyi Xue +3 more
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International Journal of Urban and Regional Research, Volume 50, Issue 1, Page 191-220, January 2026.Abstract Henry George advocated for capturing land value increases for public ends. The active approach of public authorities organizing and financing land development can help capture higher land value increases, as Hartman and Spit indicate. However, this approach hardly happens in developing countries, where the coalition of private developers and ...
Nannan Xu
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, 1997 摘 要 利用逆转录病毒载体pLNSX 构建了带HSV-TK 基因的逆转录病毒重组体pLNS - TK ,用磷酸钙沉淀法 转染PA317 包装细胞, 经G418 筛选抗性克隆, 建立了产生重组病毒的载体产生细胞系PA317/ TK, 用NIH3T3 细胞测 定病毒(CFU)滴度为3 ×108 L-1。提取PA 317/ TK 细胞的DNA 和细胞上清液的RNA, 在特异性引物引导下, 分别用 PCR 和RT-PCR检测证实PA317/ TK 细胞整合了HSV-TK 基因并产生重组病毒, 为HSV ...
谢文练 梁正东 吕 凌
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Integrative Conservation, Volume 4, Issue 4, Page 600-615, December 2025.Only 1541 (34.2%) of 4503 recognized non‐passerine bird species are held in ZIMS institutions worldwide. Approximately ~83% of the species kept globally are classified as non‐threatened and ~16% as threatened. To improve the conservation of threatened birds, a shift toward keeping threatened species should be considered within ex situ management ...
Anna Wahle +6 more
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Integrative Conservation, Volume 4, Issue 4, Page 569-583, December 2025.Accurate species identification is critical for conserving endangered Firmiana trees in China. However, morphological similarities among closely related Firmiana species complicate its taxonomic identification. We conducted genome skimming on 62 samples representing all 10 Chinese species and discovered nrITS as the optimal DNA barcode, achieving 100 ...
Li‐Jun Yan +8 more
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霍乱弧菌新发菌株中
, 2022 目的探索霍乱弧菌新发菌株H1的六型分泌系统（T6SS）存在的转录调控机制。方法本研究以转录组测序为基础，分析H1株分别由于vipA敲除（H1ΔvipA），vipB敲除（H1ΔvipB）和clpV敲除（H1ΔclpV）时T6SS基因簇的转录水平，并对其差异基因进行了基因本体论（GO）富集和京都基因与基因组百科全书（KEGG）富集。结果发现H1ΔvipA有22个差异基因（FDR<0.01，|log2FC|≥1），其中有18个属于T6SS基因簇；并且除vipA外，其转录水平均呈上升趋势 ...
张幸鼎, 赵文婧, 吴文轩, 郭扬
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The Genome of Phoebe zhennan (Lauraceae) and Comparative Genomics of Phoebe 楠木(樟科)基因组与楠属植物比较基因组学研究
Integrative Conservation, Volume 4, Issue 4, Page 584-599, December 2025.Phoebe zhennan is a Chinese endemic and valuable timber species of Phoebe (Lauraceae). We present a chromosome‐level genome assembly of P. zhennan. Our research provides novel insights into the genome evolution of Phoebe, including whole‐genome duplication, phylogenetic relationships of magnoliids, and key MYB regulatory genes involved in lignin ...
Mao Yameng +3 more
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Integrative Conservation, Volume 4, Issue 4, Page 527-533, December 2025.The recommendations cover four key aspects namely the definition of the taxa require high priority in the biodiversity conservation management, enhanced flexibility and adaptivity of the implementation processes, enforced integration of research advancement to enable the sustainable usage of plant resources, and finally actions to propose transboundary
Shi‐Cheng Shao +4 more
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Hydrocortisone Aceponate for Chronic Otitis: Long‐Term Efficacy
Veterinary Dermatology, Volume 36, Issue 6, Page 870-878, December 2025.Effective management of otitis externa (OE) requires addressing all contributing factors to prevent chronicity and recurrence. Evidence on the long‐term efficacy of topical corticosteroids in preventing recurrence and secondary infections remains limited. This retrospective noncontrolled study evaluated the efficacy of hydrocortisone aceponate (HCA) in
C. Lorente‐Méndez, D. Alonso‐Miguel
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