Results 41 to 50 of about 11,374 (168)

Updated insights into the molecular pathogenesis of canine atopic dermatitis

open access: yesVeterinary Dermatology, Volume 36, Issue 4, Page 375-384, August 2025.
Abstract Atopic dermatitis (AD) is a common and chronic inflammatory skin disease with frequent relapses. The genomics revolution has greatly contributed and revolutionised our knowledge of human AD; understanding the molecular skin fingerprint of AD and associated pathogenic immune pathways has led to preclinical assessments of several novel ...
Frane Banovic
wiley   +1 more source

Analysis of Dvl function and expression patterns of Wnt/β-catenin signaling genes in amphioxus [PDF]

open access: yes, 2015
Wnt/β-catenin信号通路是后生动物中一条相对保守的信号通路,广泛参与早期胚胎体轴的建立以及多种器官的形态建成与分化。该信号通路在很多物种中都已有研究,但是在脊索动物文昌鱼中该信号通路的研究才刚刚开始,研究的内容仅包括Wnts、β-catenin、Tcf、Axin、Dkks和sFRPs基因的表达谱分析以及化学试剂处理对胚胎发育的影响,在文昌鱼中关于该信号通路中很多重要基因的功能研究尚未开始。 本文鉴定和克隆了文昌鱼Wnt/β-catenin信号通路中的Dvl(Dishevelled)基因 ...
王净
core  

Evaluating use of two‐step International Ovarian Tumor Analysis strategy to classify adnexal masses identified in pregnancy: pilot study

open access: yesUltrasound in Obstetrics &Gynecology, Volume 64, Issue 6, Page 808-817, December 2024.
ABSTRACT Objectives The primary aim was to validate the International Ovarian Tumor Analysis (IOTA) benign simple descriptors (BDs) followed by the Assessment of Different NEoplasias in the adneXa (ADNEX) model, if BDs cannot be applied, in a two‐step strategy to classify adnexal masses identified during pregnancy. The secondary aim was to describe the
J. Barcroft   +8 more
wiley   +1 more source

Studies on Biology of Early Developmental Stages of Babylonia [PDF]

open access: yes, 2016
东风螺(Babylonia)隶属软体动物门(Mollusca)、腹足纲(Gastropoda)、新腹足目(Neogastropoda)、东风螺科(Babyloniidae),分布于中国东海、南海以及东南亚、日本和印度洋沿岸,因肉质鲜美、市场接受度高,而且生长速度快、养殖产量大,被认为是本世纪最具开发前景的优质海产腹足类之一。方斑东风螺和泥东风螺是我国常见的东风螺种类,对于其早期发育阶段的形态学以及多种组学进行研究,不仅在理论研究上具有科学意义,而且在生产实践上也具有重要的指导意义 ...
沈铭辉
core   +1 more source

Rapid, easy, sensitive, low‐cost and on‐site detection of environmental DNA and RNA using CRISPR‐Cas13

open access: yesMethods in Ecology and Evolution, Volume 15, Issue 8, Page 1408-1421, August 2024.
Abstract Environmental DNA (eDNA) monitoring of species distribution has become a critical tool in ecology, conservation biology and fisheries for identifying the presence and distribution of diverse organisms, including important, threatened and invasive species.
Jiwei Yang   +4 more
wiley   +1 more source

逆病毒介导EGFP基因转染人骨髓间充质干细胞的实验研究

open access: yesZhongguo shiyan zhenduanxue, 2009
目的探讨逆转录病毒介导增强型绿色荧光蛋白(EGFP)转染人骨髓间充质干细胞(hBM-MSC)的可行性。方法携带EGFP的重组逆转录病毒载体(EGFP-RV)转染体外培养扩增的hBM-MSC,嘌呤霉素筛选,获得表达EGFP的hBM-MSC/EGFP,以未转染的hBM-MSC为对照组,观察两组细胞的形态、生长特性、表面分子表达及向脂肪细胞、成骨细胞分化的潜能。结果EGFP-RV转染hBM-MSC后,获得稳定表达EGFP的hBM-MSC/EGFP;hBM-MSC/EGFP的细胞形态、生长特性 ...
马俐君   +4 more
doaj  

水产动物重要经济性状的分子基础及其遗传改良 [PDF]

open access: yes, 2012
中国水产养殖的成就已被认为是中国对当今世界发展的一个重大贡献.近20年来,基因组学和其他遗传技术的进步显著推动着水产动物分子生物学和遗传育种学基础研究的发展.进入21世纪后,一些主要水产动物的全基因组测序已经完成或接近完成.本文综述了包括基因组技术、体细胞核移植和干细胞技术在内的水产遗传改良技术的一些重要突破性进展,概述了包括生殖、性别、生长、抗病、耐寒和耐低氧这些重要经济性状的分子基础,列出了一系列与这些经济性状相关的候选基因.最后,介绍了几个特别是中国水产动物遗传改良在水产养殖中的应用事例 ...
朱作言, 桂建芳
core  

A global protection gap: Migrant insecurity in Mexico

open access: yesLatin American Policy, Volume 15, Issue 1, Page 129-155, March 2024.
Abstract Over the last decade, Mexico has gone from being a major source of immigrants to an important transit and destination country for asylum seekers and migrants from Central and South America. When President Andrés Manuel López Obrador took office in December 2018, he pledged to implement a migration policy that prioritized humanitarian ...
Laura Gómez‐Mera
wiley   +1 more source

人类端粒酶活性重组及其重组特异性

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2000
【目的】对Hela 细胞端粒酶进行活性重组并探讨其重组特异性。【方法】以RT-PCR 法从纯化的人端粒酶复合 体中扩增人类端粒酶RNA(hTR)基因片段, 将其克隆至含有SP6 启动子的pGEM3f +质粒中, 构建hTR 的体外转录体系以获 得体外合成的hTR。与端粒酶蛋白组分进行活性重组。四膜虫端粒酶RNA 及16S rRNA 作为重组反应对照组, 以探测其重 组特异性。【结果】经RT-PCR 扩增出450 bp 片段;体外转录重组载体经酶切图谱和PCR 扩增鉴定构建成功;体外转录的 ...
周俊宜, 罗超权
doaj  

Proteomic Response of Prorocentrum donghaiense to Phosphorus Depletion and Resupplement [PDF]

open access: yes, 2015
磷(Phosphorus,P)是海洋浮游植物生长的限制因子,影响海洋初级生产力。东海原甲藻(Prorocentrumdonghaiense,P.donghaiense)是引起我国近海有害藻华的主要甲藻。目前有关磷对甲藻生长影响的研究已有很多报道,但主要集中在生理生态层面,对甲藻在磷限制条件下及其补充响应的分子机理方面了解较少。 本论文以P.donghaiense为研究对象,运用基于质谱的定量蛋白质组学技术—串联质谱标记(Tandemmasstag,TMT),并结合相应的转录组数据,比较研究了P ...
袁春娟
core  

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