Results 121 to 130 of about 16,898 (170)

重组人高密度脂蛋白B族I型清道夫受体基因腺相关病毒血 清型2/1杂合载体的构建及其在HepG2肝细胞的表达

, 2009
【目的】 构建含绿色荧光蛋白(EGFP)重组人高密度脂蛋白B族I型清道夫受体(SR-BI)基因腺相关病毒(rAAV)2/1杂合载体,观察其在HepG2肝细胞的表达65377;【方法】以pCMV.SPORT6-SR-BI质粒为模板PCR扩增目的基因cDNA,将其克隆至pSNAV2.0-IRES-EGFP-LacZa载体中,经酶切及测序鉴定后,脂质体介导转染BHK21细胞,经G418筛选得到含SR-BI基因的BHK/SR-BI-IRES-EGFP载体细胞株,用携带rep2-cap1基因的辅助病毒(rHsv ...

core  

人鼻咽癌DNA对Rat-Ⅰ细胞株的转化活性及其基因表达的研究

Zhongshan Daxue xuebao. Yixue kexue ban, 1990
为了解EB病毒基因组阳性人鼻咽癌DNA在转染正常细胞后可能发生的生物学作用,作者将人鼻咽癌活检组织的DNA（EB病毒基因组阳性）,用磷酸钙方法转染正常Rat-Ⅰ细胞,使其恶转;用软琼脂培养的转化细胞能贴壁生长,并形成克隆;转化细胞易与PHA发生凝集;克隆接种裸鼠形成纤维肉瘤;转化细胞和裸鼠肿瘤组织用抗C3补体免疫荧光法检测EBNA呈阳性;染色体原位杂交显示在某些细胞中期染色体上有银粒出现。
doaj  

[Mechanobiology of peripheral nerve development: mechanical properties, sensing, and response]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Liu T, Lu L, Wang Y.
europepmc   +1 more source

[Efficacy of CD4/TGF-β bispecific antibody in a mouse model of peritoneal metastasis of malignant melanoma]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Jiang Q, Liu T, Wang Z, Jiang S, Wu H, Lin Y, Shiying LU, Chen L, Zhang J, Tang L, Raut RK, Xu Z.   +11 more
europepmc   +1 more source

[ATPase H⁺ transporter expression with prognosis in hepatocellular carcinoma]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Yuan Q, Jin YQ, Cao YX, Cheng LJ, Fan HM, Liu Y, Yang L.   +6 more
europepmc   +1 more source

[A real-world study of clinical cure in children with HBeAg-positive chronic hepatitis B from the Guangxi region]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Lyu RY, Tian JH, Huang SM, Meng ML, Xiang WY, Fan PY, Li J, Li SX.   +7 more
europepmc   +1 more source

[Targeted inhibition of miR-503 upregulates Apelin expression to alleviate myocardial infarction in mice]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Li J, He Y, Bi F, Chen J, Fan Z, Zhang Y, Chen E, Xiao H, Wu Y, Tian H, Zhou Y.   +10 more
europepmc   +1 more source

[SENP1 reduces ferroptosis of thyroid cancer cells by regulating hypoxia-inducible factor-1α]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhu F, Yan N, Yang J, Chen X, Wang Y.
europepmc   +1 more source

[Ophiopogonin D alleviates doxorubicin-induced myocardial hypertrophy in mice by activating the β-catenin/FUNDC1/mitophagy axis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Lei Y, Song J, Xu L, Liu R, Zhao Y.
europepmc   +1 more source

[Effect of Golgi membrane protein 1 on the proliferation, migration, and invasion of oral squamous cell carcinoma cells and its mechanism]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Li S, Wen C, Yu L, Chen J, Feng H.
europepmc   +1 more source