~(12)C~(6+)离子诱导TNFα基因在SMMC-7721细胞中的表达
, 2006 目的用Polyfect转染试剂将重组质粒pEgr-TNFα转染人肝癌细胞SMMC-7721,研究该重组质粒经12C6+离子作用后在细胞中的表达。方法用ELISA方法检测TNFα的蛋白表达,用RT-PCR方法检测TNFα转录水平的变化。结果转染pEgr-TNFα后在SMMC-7721细胞中TNFα的蛋白表达量为2·06ng/ml,2Gy12C6+离子照射后,表达量增高为2·11ng/ml,明显高于未转染组和转染空载体组;RNA转录水平也明显增高,2Gy12C6+离子照射后增高更明显。结论12C6 ...
李文建, 刘建香, 阮健磊, 苏旭
core
[Influence and mechanism of exosomes derived from rat bone marrow mesenchymal stem cells on rat Fbs under high glucose conditions]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiWu Y, Yin WH, Yu M, Wang D, Wu J.
europepmc +1 more source
The Biological Effect of Exogenous Wild Type p53 Combined with Carbon-Ion Beam on Tumor Cell Lines
, 2007 肿瘤是严重威胁人类生命健康的常见病、多发病，不仅病因复杂、发生发展异常迅速，而且到目前为止，发病机理不完全清楚，尚无适应范围广和有特异疗效的治疗方法。因此，肿瘤治疗方法的探索依然是医学、生物学及其相关学科研究的热点。肿瘤的重离子治疗和基因治疗是近年来发展起来的新的肿瘤治疗方法。但它们同样或多或少存在一些不足。在肿瘤治疗方法的探索中，将两种或两种以上理化特性或生物学作用原理不尽相同的现有治疗方法有机结合，充分利用各自优势，取长补短，使治疗效果叠加，对肿瘤发挥协同或相加抑制作用 ...
刘兵
core
[ZEB2 promotes glioblastoma progression by reprogramming lipid metabolism through the CYLD/FASN axis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoChen Z, Chai P, Mao Y, Liu R, Song Y.
europepmc +1 more source
[A case of neonatal fatty acid oxidation disorder]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiDeng LL +4 more
europepmc +1 more source
, 1998 目的: 研究小鼠 B7 基因转染对小鼠肝癌细胞 Hepal-6 细胞株细胞形态、生长速度及致瘤性的影响。方法: 用逆转录病毒载体,将 小鼠B7 基因导入小鼠肝癌细胞 Hepal-6 细胞株中,经G418 筛选,获得高表达 B7分子的阳性细胞克隆,采用CTLA-4Ig 的间接免疫荧光法检测 B7 分子的表达情况,并观察了B7 基因转染对细胞形态、生长速度及致瘤性的影响。结果:①亲本小鼠肝癌细胞株 Hepal-6 不表达 B7 分子,转 染 B7 基因的 Hepal-6 细胞高表达 B7 分子;②转染 B7
core
[Overexpression of miR-593-5p inhibits migration, invasion and proliferation and promotes apoptosis of gastric cancer cells by targeting PLK1]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoDong B, Peng J, Li J, Meng Y, Yang X.
europepmc +1 more source
[Menstrual blood-derived mesenchymal stem cell transplantation improves anti-fibrosis mechanisms in mouse models of alcoholic liver fibrosis]. [PDF]
Zhonghua Gan Zang Bing Za ZhiYang C +7 more
europepmc +1 more source
[Application of cell transfer technology in pathological diagnosis of micro-volume cell fluid]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZhao Y, Diao X, Xiong Y.
europepmc +1 more source
[Effect of LncRNA DANCR on the immune microenvironment of glioma cells by regulating the miR-656/BMPR1A axis]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanWang O, Zhu P, Lin J, Wu H.
europepmc +1 more source