RNAi-chk1在抑制小鼠Lewis肺癌细胞生长作用的实验研究
Zhongguo shiyan zhenduanxue, 2012 目的观察chk1-RNAi联合放疗对Lewis肺癌细胞的抑制及凋亡情况。方法以脂质体Lipofectimine-2000介导,将chk1-536的siRNA表达载体转染小鼠Lewis肺癌细胞株,将其分为空白对照组、错义序列组、siRNA组,2Gy照射组、2Gy照射+错义序列组、2Gy照射+siRNA组。应用MTT法检测转染细胞抑制率、流式细胞术分析转染siRNA后细胞的凋亡,并应用RT-PCR检测转染细胞chk1-mRNA表达水平。结果 chk1-siRNA转染联合辐射组的Lewis肺癌细胞 ...
侯吉光 +4 more
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘 要： 【目的】 研究ｍｉＲ－１０３对肝癌细胞增殖功能的影响及其作用机制。【方法】 Ｒｅａｌ ｔｉｍｅ ＰＣＲ方法检测ｍｉＲ－１０３在肝癌组织和细胞中的表达。将ｍｉＲ－１０３ｉｎｈｉｂｉｔｏｒ转染到肝癌细胞中，分为实验组（转染ｍｉＲ－１０３ ｉｎｈｉｂｉｔｏｒ）和对照组（转染Ｎｅｇａｔｉｖｅ Ｃｏｎｔｒｏｌ），通过ＭＴＴ实验检测肝癌细胞的增殖变化。Ｒｅａｌｔｉｍｅ ＰＣＲ和Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ检测转染ｍｉＲ－１０３ ｍｉｍｉｃｓ后肝癌细胞中ＦＢＷ７ ｍＲＮＡ和蛋白的表达变化 ...
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人类免疫缺陷病毒-1 nef诱导凋亡及下调人类白细胞抗原-Ⅰ类分子
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 [目的]研究人类免疫缺陷病毒（HIV）-1 nef调节细胞表面的人类白细胞抗原（HLA）-Ⅰ类分子及诱导细胞凋亡情况。[方法]将293T细胞分为两组,用脂质体法将含HIV-1 nef基因的质粒及空载体分别转染细胞,Western blot检测HIV-1 nef在转染细胞中的表达,荧光抗体染色结合流式细胞术检测细胞表面的HLA-I类分子表达及细胞凋亡情况。[结果]HIV-1 nef在转染细胞中有表达。转染HIV-1 nef的细胞与转染空载体的细胞经抗HLA-A,B,C-FITC染色后 ...
黄官友 +4 more
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Hemodialysis bilayer bionic blood vessels developed by the mechanical stimulation of hepatitis B viral X(HBX) gene- transfected hepatic stellate cells. [PDF]
J Zhejiang Univ Sci BLiu H +10 more
europepmc +1 more source
rAAV1-EGFP和rAAV2-EGFP转染犬骨髓间充质 干细胞的比较
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 研究不同血清型腺相关病毒(rAAV)载体介导的增强绿色荧光蛋白基因(EGFP)对体外培养犬骨髓间充质干细胞(MSC)的转染效率及其毒性作用65377;【方法】 应用密度梯度离心法及贴壁筛选法分离培养犬MSC,倒置显微镜及Giemsa染色观察细胞形态,透射电镜观察其超微结构,流式细胞仪鉴定其表面标记物65377;取第3代MSC,以MOI为104 ~ 105 v.g./cell 的rAAV1-EGFP 和rAAV2-EGFP进行转染,3 d65380;7 d65380;21 ...
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[Application of cell transfer technology in pathological diagnosis of micro-volume cell fluid]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZhao Y, Diao X, Xiong Y.
europepmc +1 more source
[Effect of Golgi membrane protein 1 on the proliferation, migration, and invasion of oral squamous cell carcinoma cells and its mechanism]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiLi S, Wen C, Yu L, Chen J, Feng H.
europepmc +1 more source
[Dihydroartemisinin inhibits the progression of oral squamous cell carcinoma]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiShan T +5 more
europepmc +1 more source
[Advances in the construction and application of a novel liver organoid model for hepatitis B virus infection]. [PDF]
Zhonghua Gan Zang Bing Za ZhiLi H, Xiang KH.
europepmc +1 more source
[miR-488-5p promotes osteogenic and neurogenic differentiation of rat bone marrow mesenchymal stem cells and enhances neuralized bone regeneration]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZeng L +5 more
europepmc +1 more source