Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 构建携带有增强型绿色荧光蛋白(EGFP)基因的逆转录病毒载体,观察GFP的表达情况以及对靶细胞生物特性的影响65377; 【方法】 以质粒pEGFP-C1为模板进行PCR反应,获得完整保留pEGFP-C1载体多克隆酶切位点(MCS)的EGFP基因片段,定向插入逆转录病毒载体pLXSN,筛选出正确的重组逆转录病毒载体pLXSN-EGFP,转染PA317包装细胞后获得具有感染能力的病毒颗粒,转染人多型性脑胶质瘤细胞SW038-C2,通过荧光显微镜和流式细胞仪(FACS ...
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[Immunoadjuvant Effect of Chitosan Oligosaccharide and Its Feasibility of Being Used as an Adjuvant for Attenuated Live Bacteria Vector Vaccines]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanOu Q +8 more
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[A simulation study on the differences in compressive mechanical properties of Voronoi porous structures with two strut-diameter morphologies]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiMen Y, Wang T, Wu M, Zhang C.
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, 2016 本发明提供一种模拟循环载荷下板锚极限动承载力的测试系统，包括：板锚模型、用于将板锚模型的锚胫限定为垂直于床面移动的十字支撑架、循环载荷加载装置和测量装置；循环载荷加载装置用于向锚胫施加循环载荷，包括将电机输入的匀速转动转换为模拟正弦的往复转动的传动装置、将往复转动转化为指定常荷载与模拟正弦荷载叠加的循环荷载位移载荷转化装置和用于根据板锚模型的位移调整绳索的长度，使循环载荷的幅值不因多周期的累积板锚模型位移而发生衰减的位移修正装置。本方案能有效控制循环载荷的幅值、获得循环拉拔过程中板锚周围土体的孔隙水压 ...
胡存, 高福平, 汪宁
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Zhongshan Daxue xuebao. Yixue kexue ban, 1999 目的: 构建携带人神经生长因子( hNGF)基因的真核细胞表达载体, 为应用 NGF 进行老年性痴呆(AD)等疾病基 因治疗打基础。方法: 应用基因重组技术将 hNGF cDNA 克隆到逆转录病毒载体 pLXSN 中 ,用限制性内切酶酶切分析重组质粒 pLNGFSN 中 hNGF 基因的插入方向,用 PCR、斑点杂交和 Southern 杂交对重组质粒 pLNGFSN 作进一步鉴定。结果: hNGF cDNA 已正确地克隆到逆转录病毒载体 pLXSN 中,而构建成重组逆转录病毒载体 pLNGFSN ...
doaj
[Research progress on the application of pollen in the biomedical field]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiBai L, Wang Y, Yang L, Wang Y, Sun Y.
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[Exploring the feasibility and limitations of using large language model to interpret bibliometric findings: a case study in hemophilia gene therapy]. [PDF]
Zhonghua Xue Ye Xue Za ZhiBai Y +5 more
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[Finite element analysis of tibial fracture risk following lateral unicompartmental knee arthroplasty under varying bone conditions]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiLi Z, Huang T, Xu Y, Sun X.
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[Oral mucosal administration]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiLiu T, Liu J, Zhao H.
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[Advancement in the role of exosomes for hepatitis B virus infection diagnosis and treatment: mechanisms and clinical applications]. [PDF]
Zhonghua Gan Zang Bing Za ZhiDu MX +6 more
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